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Study on the regulation mechanism of megakaryocyte and thrombo-cytopoiesis

Research Project

Project/Area Number 61570586
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Hematology
Research InstitutionFukushima Medical College

Principal Investigator

KIMURA Hideo  The First Department of Internal Medicine, Fukushima Medical College, 内科学第一講座, 助手 (00128547)

Co-Investigator(Kenkyū-buntansha) ISHIBASHI Toshiyuki  The First Department of Internal Medicine, Fukushima Medical College, 内科学第一講座, 副手
Project Period (FY) 1986 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1988: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1987: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1986: ¥800,000 (Direct Cost: ¥800,000)
KeywordsMegakaryocytopoiesis / Thrombocytopoiesis / Essential thrombocythemia / GM-CSF / IL-6 / トロンボポエチン / CFU-meg / meg-CSA / Megakaryocyropoiesis / 各種造血因子 / 巨核球・血小板産生 / 巨核球コロニー刺激因子 / CFU-Meg / Percoll連続密度勾配 / 原発性血小板血症
Research Abstract

Megakaryocytopoiesis in essential thrombocythemia (ET) and polycythemia vera (PV)
To characterize abnormal megakaryocytopoiesis and thrombopoiesis in ET and PV, bone marrow megakaryocytic progenitors (CFU-MK) were cultured in a methylcellulose system containg human plasma. Most of the ET patients and about two-thirds of the PV patients studied revealed spontaneous CFU-MK colony growth in the absence of stimulator (PHA-LCM). The number of CFU-MK in ET and PV was significantly increased than that of the control subjects. CFU-MK from ET and PV gave rise to larger number of megakaryocytes per each colony compared to the control subjects. Among PV pateints, those who showed spontaneous CFU-MK were associated with an increased megakaryocyte number number on marrow biopsy specimen and thrombocytosis. Patients with reactive thrombocytosis showed no spontaneous CFU-MK growth, similar to the contro subjects. The data suggested that the abnormally incresed number of megakaryocytes and resultant th … More rombocytosis is, at least partly, due to such abnormal CFU-MK population (spontaneous CFU-MK; autonomous or highly sensitive population to the stimulator). Reactive thrombocytosis may be due to some stimuli on post-CFU-MK compartment (possibly due to incresed thrombopoietin).
Efect of various pure growth factors on murine megakaryocytopoiesis
The effects of erythropoietin and IL-3 on murine megakaryocytopoiesis in vitro have been previously demonstrated. This time, we studied the effect of GM-CSF (mouse recombinant) on murine megakaryocytopoiesis in vitro and in vivo. The effect of IL-6 (human, recombinant) was also studied.
GM-CSF stimulated CFU-MK as well as CFU-GM colony formation in serum depletedagar culture. GM-CSF also increased the size of megakaryocytes and acetylcholinesterase activity of megakarocytes in liquid culture. The effect was found direct using a single megakaryocyte cylture with or without GM-CSF. When injected in vivo (1 ug twice a day, each mouse, for 6 days), two fold increase of granuloctes was observed. CFU-MK numbers in bone marrow and platelet level were not changed.
Using the same culture systems, UL-6 was found to promote the maturation but not proliferation of murine megakaryocytes. The effect was direct. The in vivo role of IL-6 remains to be studied. Less

Report

(4 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1987 Annual Research Report
  • 1986 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] Kimura H.et al: Acta Haematol. 79. 1-6 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kimura H.: Acta Haematologica Jpn. 49. 1696-1704 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Shiga Y.;Kimura H.et al.: Leukemia Research. 11. 711-718 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 大越透、木村秀夫 他: 日本血液学会雑誌. 51. 606-611 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kimura H; Ishibashi T; Sato T; Matsuda S; Uchida T; Kariyone S:"Megakaryocytic colony formation (CFU-Meg) in essential thrombocythemia: Quantitative and qualitative abnormalities of bone marrow CFU-Meg" Am J Hematol. 24. 23-30 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kimura H; Ohkoshi T; Matsuda S; Uchida T; Kariyone S:"Megakaryocytopoiesis in polycythemia vera: Characterization by megakaryocytic progenitors (CFU-Meg) in vitro and quantitation of marrow megakaryocytes" Acta Haematologica. 79. 1-6 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Shiga Y; Kimura H; Abe R; Sato T; Matsuda S; Uchida T; Kariyone S; Maseki N; Sakurai M:"Chromosome studies of hematopoietic colonies for distinct diagnosis of megakaryoblastic crisis of chronic myelogenous leukemia: A case report" Leukemia Research. 11. 711-718 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ishibashi T; Kimura H; Shikama Y; Uchida T; Kariyone S:"Effect of recombinant granulocyte-macrophage colony-stimulation factor on murine thrombocytopoiesis in vitro and in vivo"

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ishibashi T; Kimura H; Uchida T; Kariyone S; Friese P; Burstein SA:"Human interleukin 6 is a potent direct promotor of maturation, but not proliferation of megakaryocytes in vitro"

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kimura,H.,et al: Acta Haematol. 79. 1-6 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Kimura,H.: Acta Haematologica Jpn. 49. 1696-1704 (1986)

    • Related Report
      1988 Annual Research Report
  • [Publications] Shiga,Y.;Kimura,H.,et al: Leukemia Research. 11. 711-718 (1987)

    • Related Report
      1988 Annual Research Report
  • [Publications] 大越透,木村秀夫 他: 日本血液学会雑誌. 51. 606-611 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Kimura H. et al: Am J Hematol. 24. 23-30 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 志賀隆, 木村香夫 他: 日本血液学会雑誌. 50. 1358-1363 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Shiga Y. ;Kimura H et al: Leukemia Research. 11. 711-718 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 木村秀夫 他: 医学のあゆみ. 142. 501-502 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Kimura H et al: Acta Haamertol. 79. 1-6 (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] 大越透, 木村秀夫 他: 日本血液学会雑誌. (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] Kimura H: Acta Haematologica Jpn. 49. 1696-1704 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Shiga Y;Kimura H et al.: Leukemia Research. (1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] Kimura H et al.: American J Hematology. 24. 23-30 (1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] 志賀隆,木村秀夫,他: 日本血液学会誌. (1987)

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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