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Studies on regulation of hematopoieses by bone marrow stromal cells

Research Project

Project/Area Number 61570592
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Hematology
Research InstitutionTokai University

Principal Investigator

TADAMI Nagao  School of Medicine, Tokai University, 医学部, 教授 (20093693)

Co-Investigator(Kenkyū-buntansha) KUNIHIKO Yamauchi  School of Medicine, Tokai Univeraity, 医学部, 講師 (70130101)
Project Period (FY) 1986 – 1987
Project Status Completed (Fiscal Year 1987)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1987: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1986: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordsBone marrow stromal cells / Bone marrow fibroblasts / Bone marrow fat cells / Endothelial cells / Regulation of hematopoiesis / 造血幹細胞
Research Abstract

1. It has been investigated that bone marrow stromal cells consisting of fibroblasts, fat cells and endothelial cells influence the differentiation and proliferation of hematopoietic stem cells. Bone marrow fibroblasts and fat cells were obtained from 10 healthy volunteers and patients with 10 acute leukemia, six chronic mylogenous leukemia and 10 aplastic anemia. Endothelial cells cultured from umbilical cord veins. Conditines-medium was obtained from these stromal cells.
2. It has been revealed that fibroblasts regulate granulopoiesis with cell-to-cell interaction and cell-mediated liquid factor. It is considered that granulocytopenia in acute leukemia and aplastic anemia is due to disturbed regulation of granulopoiesis by fibroblasts and that leukocytosis in chronic myelogenous leukemia is due to dysfunction of inhibitory factor of fibroblasts on granulopoiesis. Fat cells did not influence granulopoiesis. Endothelial cells proved importaht cells which release granulocyte colony-stimulating factor.
3. Fibroblasts inhibited erythropoiesis, but fat cells obtained from fibroblasts incubated with steroid hormone did not inhibit erythropoiesis. Fibroblasts derived from patients with aplastic anemia inhibited erythropoiesis to a greater extent than those from normal subjects and the degree of recovery from inhibition by fat cells from the patients was significantly greater than those from normal individuals.
4 Fibroblasts inhibited megakaryocyte and mixed myeloid lineage colont formation. Fat cells and endothelial cells did not influence these colony formations.
5 The inhibitory factor obtained from fibroblast conditioned-medium was heat-resistant and its molecuar weight was 1X10^4 - 5X10^4 dalton.
6 These results show that different components of stromal cells such as fibroblasts have contrasting effects on hematopoiesis in the bone marrow and they are useful findings for unerstanding pathogenesis of acute leukemia and aplastic anemia.

Report

(2 results)
  • 1987 Final Research Report Summary
  • 1986 Annual Research Report

Research Products

(10 results)

All Other

All Publications (10 results)

  • [Publications] 長尾忠美,山内邦彦,清水正樹,野口憲一: Experimental Hematology. 14. 696-701 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 長尾忠美: Tokai Journal of Experimental and Clinical Medicine. 12. 1-6 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 清水正樹,長尾忠美,有森茂: Tokai Journal of Experimental and Clinical Medicine. 12. 67-72 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Nagao T, Yamauchi K, Shimizu M, Noguchi K: "Regulatory role of human bone marrow fibroblasts in proliferation by granulocyte and mecrophage colony-forming cells" Experimental Hematology. 14. 696-701 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Nagao T: "Characteristics of bone marrow fibroblasts and their significance in hematopoiesis" Tokai Journal of Experimental and Clinical Medicine. 12. 1-6 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Shimizu M, Nagao T, Arimori S: "Abnormal regulation of granulopoiesis by bone marrow fibroblasts in leukemia" Tokai Journal of Experimental and Clinical Medicine. 12. 67-72 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Experimental Hematology. 14. 696-701 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Tokai Journal of Experimental and Clinical Medicine. (1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] Experimental Hematology. (1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] Tokai Journal of Experimental and Clinical Medicine. (1987)

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-30   Modified: 2016-04-21  

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