| Project/Area Number |
61570593
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Hematology
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| Research Institution | Tokai University |
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Principal Investigator |
JUNKO Matouchi Tokai University, School of Medice, Instructor, 医学部, 助手 (40125414)
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| Co-Investigator(Kenkyū-buntansha) |
TETSUYA Moriuchi Tokai University, School of Medicine, Lecturer, 医学部, 講師 (20174394)
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| Project Period (FY) |
1986 – 1987
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| Project Status |
Completed (Fiscal Year 1987)
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| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1987: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1986: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | oncogens / leukrmia / thymoma / non-radioactive histo- in situ hybridization / c-myc / c-k-ras / in situ hybridization |
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| Research Abstract |
Oncogene abnromalities are thought to have a central role in hematopoietic neoplasias. However, the extent to which specific oncogene changes determine the clinical features of the disorders is unknown. Our study planned to inverstigate leukemic cells if there are abnormal amount if informations and products of oncogenes by using non-radioactive histo-in sity hybridization with cDNA probes and by antibodies to oncogene products. Leukemic cells from 30 patients were classified according to the FAB classification, and subjectes in this study. We also studied the expression of oncogenes in 28 thymomas (epithelial type 7, mixed type 14, lymphocytic type 7). The results of our studies can be summatised as follows: 1) The amount of c-myc oncogene products were significantly increased in 2 of 4 cases with promyelocytic leukemia (M3) and also in 2 of 4 with myelomonocytic leukemia (M4). No significant inctrase was observed in other type of luqkimic cells including 10 cases of acute myeloblasti
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c leukemia (M1 and M2), 4 acute lymphoblastic leukemia and 4 chronic myelocytic leukemia. 2) The cK-res gene product was inctrased in leukemic cells from 2 patients with M3. The leukemic cells from one of these 2 patients also expressed much amount of c-myc gene products indicating the possibility of cooperation of two oncogene products on the onset of leukemia. 3) Increased information and products of the c-myc gene were observed in several thymomas. The positive frequency of c-myc gene expression was not different depend on its histological type. In contrast increased amount of c-k-ras gene products were observed in only 2 thymomas of epithelial type. 4) By non-radioactive histo-in sity hybridizxation method, mRNAs of ATL cells were examined. When c-myc cDNA of HTLV-I DNA was used as a probe, about 25% of the cells were stained. And over 70% of the cells were positive when mouse T cell receptor b chain cDNA was used as a probe. These results are consistent with the clinical and hematological features suggesting the usefullness of non-radioactive histo-in sity hybridization. Less
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