| Project/Area Number |
61570595
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Hematology
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| Research Institution | Hyogo College of Medicine |
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Principal Investigator |
KANAMARU Akihisa Hyogo College of Medicine, 医学部, 講師 (70068534)
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| Co-Investigator(Kenkyū-buntansha) |
OKAMOTO Takahiro Hyogo College of Medicine, 医学部, 助手 (80177089)
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| Project Period (FY) |
1986 – 1987
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| Project Status |
Completed (Fiscal Year 1987)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1987: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1986: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Interleukin 3 / Graft-versus-Host disease / Hematopoietic progenitors / 抑制因子 / 造血因子 / インターロイキン-3 / エンドトキシン |
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| Research Abstract |
We have demonstrated a high level of interleukin 3(IL-3) in the serum of mice with graft-versus-host disease(GVH-D).The level of IL-3 rised as the GVH-D became severer.The level of this factor was measured by the method of either erythroid colony-formation from 14 day-fetal mouse liver cells or^3H-TdR uptake in FDC-P2 cells which are IL-3 dependent cell line.The inhibitory activity was detected in the serum by the method using FDC-P2 cells but not erythroid colony-forming technique.However,the specificity or physiological role of this activity was obscure.The possibility that the hematopoietic activity of the serum might be due to a factor induced by endotoxin was excluded by the experiments with endotoxin non-responder C3H/Hej mice,in which the activity was elevated as much as the control mice when suffering from GVH-D.The production of IL-3 was observed in liquid culture media from bone marrow,spleen and lymph node cells in GVH-D mice.The in vivo effect of IL-3 was confirmed in mice injected with the serum from polycytemic GVH-D mice, in which serum erythropoietin level was reduced into undetectable range.Total numbers of BFU-E,CFU-C and CFU-Mix were remarkably increased in spleens of the mice 3 days after the injection.IL-3 in the serum stimulated the proliferation and differentiation of the hematopoietic progenitors in mice. Consequently,it plays and important role in hematopoiseis in vivo as well as in vitro.
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