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In vivo production and hematopoietic activity of interleukin 3 (IL-3) in mice

Research Project

Project/Area Number 61570595
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Hematology
Research InstitutionHyogo College of Medicine

Principal Investigator

KANAMARU Akihisa  Hyogo College of Medicine, 医学部, 講師 (70068534)

Co-Investigator(Kenkyū-buntansha) OKAMOTO Takahiro  Hyogo College of Medicine, 医学部, 助手 (80177089)
Project Period (FY) 1986 – 1987
Project Status Completed (Fiscal Year 1987)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1987: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1986: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsInterleukin 3 / Graft-versus-Host disease / Hematopoietic progenitors / 抑制因子 / 造血因子 / インターロイキン-3 / エンドトキシン
Research Abstract

We have demonstrated a high level of interleukin 3(IL-3) in the serum of mice with graft-versus-host disease(GVH-D).The level of IL-3 rised as the GVH-D became severer.The level of this factor was measured by the method of either erythroid colony-formation from 14 day-fetal mouse liver cells or^3H-TdR uptake in FDC-P2 cells which are IL-3 dependent cell line.The inhibitory activity was detected in the serum by the method using FDC-P2 cells but not erythroid colony-forming technique.However,the specificity or physiological role of this activity was obscure.The possibility that the hematopoietic activity of the serum might be due to a factor induced by endotoxin was excluded by the experiments with endotoxin non-responder C3H/Hej mice,in which the activity was elevated as much as the control mice when suffering from GVH-D.The production of IL-3 was observed in liquid culture media from bone marrow,spleen and lymph node cells in GVH-D mice.The in vivo effect of IL-3 was confirmed in mice injected with the serum from polycytemic GVH-D mice, in which serum erythropoietin level was reduced into undetectable range.Total numbers of BFU-E,CFU-C and CFU-Mix were remarkably increased in spleens of the mice 3 days after the injection.IL-3 in the serum stimulated the proliferation and differentiation of the hematopoietic progenitors in mice. Consequently,it plays and important role in hematopoiseis in vivo as well as in vitro.

Report

(2 results)
  • 1987 Final Research Report Summary
  • 1986 Annual Research Report
  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] 金丸昭久: 臨床血液. 27. 1186-1194 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] A.Kanamaru et al: International Journal of cell Cloning. 5. 134-141 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] A.Kanamaru;H,Hara: International Journal of Cell Cloning. 5. 450-462 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] T,Okamoto et al: Experimental Hematology. 15. 190-194 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Akihisa Kanamaru: "Actions and in vivo role of burst-promoting activity(BPA)" The Japanese Journal of Clinical Hematology. 27. 1186-1194 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Akihisa Kanamaru,Hiroki Nakayama,Keiko Okuda,Ken Matsuda,Yukihiko Kitamura and Kiyoyasu nagai: "Characteristics of murine yolk sac erythroid progenitors and their population expansion in liquid culture" International Journal of Cell Cloning. 5. 134-141 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Akihisa Kanamaru and Hiroshi hara: "Hematopoietic factors in graft-versus-host reaction" International Journal of Cell Cloning. 5. 450-462 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Takahiro Okamoto,Akihisa Kanamaru,Hiroshi Hara and Kayoyasu Nagai: "Changes in expression of major histocompatibility complex(MHC) class-I antigen on hematopoietic progenitors during murine development" Experimental Hematology. 15. 190-194 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 篠原慶希 他: 臨床血液. 27. 30-35 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] 金丸昭久: 臨床血液. 27. 1186-1194 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] A.Kanamaru,et al.: International Journal of Cell Cloning. 5. (1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] S.Yoshiyasu,et al.: American Journal of Hematology.

    • Related Report
      1986 Annual Research Report
  • [Publications] T.Okamoto,et al.: Experimental Hematology.

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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