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INVESTIGATION ON HYBRID TYPE ARTIFICIAL LIVER USING CANINE HEPATOCELLS IN PRIMARY CULTURE

Research Project

Project/Area Number 61570598
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field General surgery
Research InstitutionHokkaido University

Principal Investigator

TUBURAYA Toshihiko  Medical Hospital, Hokkaido University, 医学部付属病院, 講師 (70142198)

Co-Investigator(Kenkyū-buntansha) KAKITA Akira  Faculty of Medicin, Hokkaido University, 医学部, 助教授 (90109439)
Project Period (FY) 1986 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1988: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1987: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1986: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsHybrid type artificial liver / hepatocyte / primary culture / total hepatectomy / ヒト-EGF / ブタ肝細胞 / 異種血清による細胞培養 / 人工肝臓 / 初代培養肝細胞 / ヒトEGF / 初代単層培養 / 肝機能 / 人工肝 / 初代肝細胞培養 / アンモニア処理能
Research Abstract

Viable hepatocytes isolated from canine liver by collagenase were cultured on the glass plates 10cm inwidth and 20cm in length coated with collagen. Long stroke type artificial liver module was constructed by pilling up the glass plates containing about 6 billions and 70-80g cells in weight almost equivalent to 1/3 of a canine liver in situ. All hepatocytes in the module have been quite viable during 2 weeks in perfusion culture and maintained various liver functions at high level. These functions have been maintained for at least 2 weeks in dog plasma as well as in culture medium suplemented with hormones.
The functions of this artificial liver module were evaluated on unhepaic dogs. Total hepatectomy was done to use silicone coated tube for portal and caval shunt. Mean survival time of treated group by the artificial liver was 53.3hours. It was extremely prolonged than that of non treated controll group of 21.5hours. Serum albumin concentration, NH_3 level and APTT activities activities revealed satisfactory conditions in treated group.
This artificial liver using primary cultured hepatocytes may pilot new development of a hybrid type artificial liver with matabolic functions.

Report

(4 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1987 Annual Research Report
  • 1986 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] 船津昭彦 他: 人工臓器. 17. 170-174 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 熊谷文昭 他: 人工肝臓. 17. 175-178 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 浜田明倫 他: 人工臓器. 18. (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 圓谷敏彦: 医学のあゆみ. (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 熊谷文昭: 日本外科学会雑誌. (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Uchino Junichi.: ASAIO transaction. (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tsuburaya Toshihiko.: ASAIO transaction. (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Funatsu Akihiko et al: "Hybrid type artificial liver using adult dog hepatocytes in primary culture" Jinkouzouki. 17. 170-174 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kumagai Fumiaki et al: "Treatment of total hepatectomized dog by a new hybrid artificial liver using primary cyltured adult dog hepatocytes" Jinkouzouki. 17. 175-178 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Hamada Tomonori et al: "Choice of hepatocytes culture media, substrata for hybrid arificial liver module and treatment of totaly hepatectomized dog" Jinkouzouki. 18. (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tsuburayatoshihiko: "Hepatic asist with isolated hepatocytes" Igakunoayumi. (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kumagai Fumiaki: "Hybrid bioartificial liver using canine hepatocytes in primary culture" J. J. S. A.(1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Uchino Junichi et al: "Bioartificial liver" ASAIO transaction. (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tuburaya toshihiko et al: "Hybrid artificial liver using primary cultured hepatocytes" ASAIO transaction. (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 浜田朋倫 他: 人工臓器. 18. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] 圓谷敏彦: 医学のあゆみ. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] 熊谷文昭: 日本外科学会雑誌. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Uchino,Junichi: ASAIO transaction. 34. 972-976 (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Tsuburaya,Toshihiko: ASAIO transaction. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] 船津 昭彦: 人工臓器. 17. 170-174 (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] 熊谷 文昭: 人工臓器. 17. 175-180 (1988)

    • Related Report
      1987 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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