Experimental Study on immunological function in splenic autotransplantation
| Project/Area Number |
61570624
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
General surgery
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| Research Institution | Teikyo University |
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Principal Investigator |
KOTA Okinaga Teikyo University School of Medicine, Professor, 医学部, 教授 (00101098)
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| Co-Investigator(Kenkyū-buntansha) |
HISAE Iinuma Teikyo Unversity school of Medicine, Instructor, 医学部, 助手 (30147102)
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| Project Period (FY) |
1986 – 1987
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| Project Status |
Completed (Fiscal Year 1987)
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| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1987: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1986: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | Splenic autotransplanation / Infection immunity / Tumor immunity Interleukin-1 / %Interleukin-2 / %lymphocyte subset / NK活性 / リンパ球サブセット / ケミルミネッセンス / 脾摘後感染 |
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| Research Abstract |
For precention of postsplencetomy infection, we studied the most effective site and amount of splenic autograft using SD rats. Rate were divided into 5 Goups to determine the most effective site omentum,intraperitoneal, intermnauscular, spelencetomy, and sham operation, And to determine the necessary amount for protection against infection the were dibvided into 7 groups: 2030mg, 50-60 mg, 100mg 200mg, 300mg, transplant splenectomy, and sham operation. Pnumococcal cleatrance from blood and survival rate were significantly higher in the mentum group and in the two groups with 200mg nd 300mg transplant then in ther other groups. These tesults suggest that the most effective site is the ometum, and approximately 50 percent of the whole spleen woulf be necssary for preventrion against sepsis. To evaluatr the function of regerated spleen, the splenci tissuw was removed four months afte transplantation. %the number of the macrophages and lymphocyted per gram of the regenerated spleen was slighty leds than that to the normal spleen. There were no singificant diffrtences in phagocytic function and clemiluminesoence of thre macroupages between the transplanted spleed and the normal spleen. The interleukin-1 activity of the amctophsages and the Interleukin-2 activity of the lymphocyted from thre transdplanted spleen were significantly dectrased compared with thr normal spleen. %the function of regenerated splenic tissus from WBN/Kob rate bearing MNNG induced gastric cancer cells were investigated. Two weeks and one month after surgeary the regenerated splenic tiuus was removed. There werre no significant differences in NK activity and interleukin-2 activity of the mononuclear cells between the tumor bearing transplanted spleen and the control transplanted spleed. And the number of supressor T cells of the lymphocyted from ther transplanted spleen was significently increased compared with the lymphocytes from the control transplanted spleen.
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Report
(2 results)
Research Products
(6 results)
