Cancer and lipid-lipid metabolism in carcinogenesis, tumor growth and metastasis -
| Project/Area Number |
61570626
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
General surgery
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| Research Institution | Kansai Medical University |
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Principal Investigator |
YAMAMOTO Masakatsu Kansai Medical University , Department of Surgery, Instructor, 医学部, 教授 (30077573)
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| Co-Investigator(Kenkyū-buntansha) |
HIRAMATSU Yoshifumi Kansai Medical University , Department of Surgery, Assistant, 医学部, 助手 (30173264)
TAKADA Hideho Kansai Medical University , Department of Surgery, Instructor, 医学部, 講師 (30131444)
YAMAMURA Manabu Kansai Medical University , Department of Surgery, Instructor, 医学部, 講師 (60077732)
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| Project Period (FY) |
1986 – 1988
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| Project Status |
Completed (Fiscal Year 1988)
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| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1988: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1987: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1986: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | polyunsaturated fatty acids / eicosapentaenoic acid / chemical carcinogenesis / tumor growth / 遠隔転移 / 科学発癌 / 遠隔移転 / ニトロソウレア誘発乳癌 / アゾキシメタン誘発大腸癌 / 食餌性脂質 / 組織脂肪酸組成 / プロスタグランジン / 多価不飽和酸 |
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| Research Abstract |
The effects of qualitative change of dietary fatty acids on mammary and colon carcinogenesis, primary tumor growth and lung metastasis of implanted Lewis lung carcinoma were studied. Rats or mice were fed three types of semipurified diet containing 4.7% stearic acid plus 0.3% linoleic acid (saturated fatty acid group), 5% linoleic acid (n-6 polyunsaturated fatty acid, (PUFA) group), 4.7% eicosapentaenoic acid (EPA, n-3 PUFA group) plus 0.3% linoleic acid. 1. On mammary carcinogenesis induced by N-nitroso-N-methylurea in Sprague-Dawley rats, EPA diet significantly reduced tumor incidence, tumor weight and tumor yields when compared to other diets. Phospholipid fatty acid composition showed n-3 PUFA may in part displaced with n-6 PUFA. 2. On colon carcinogenesis induced by azoxymethane in Donryu rats, EPA diet also reduced tumor incidence and tumor yields compared to linoleic acid diet. Phospholipid fatty acid composition showed colon tumor contained higher amount of archidonic acid which is precursor of prostaglandins(PG) than normal colon mucosa. However, EPA suppressed the excessive production of PGE_2, which may be accompanied with neoplastic production, whereas linoleic acid caused a marked increase in the tumor content of PGE_2 compared to normal colon mucosa. These result suggest that EPA exert its inhibitory effect on colon or mammar carcinogenesis by modulating lipid metabolism and inhibiting PGE_2 synthesis in tumor cells. 3. On the primary growth and lung metastasis of implanted Lewis lung carcinoma in C57BL/6 mice, EPA decreased tumor weight, volume and the number of metastatic lung nodules. In biological membranes EPA may affect functional specificity, including prostanoid synthesis, and membrane structure which influences permeability, fluidity and lipid-protein interactions. Including nutritional effect, further studies are needed to understand the complex involvement of EPA on cancer cells.
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Report
(4 results)
Research Products
(3 results)
