Purification of a growth factor from human bone matrix and its clinical application.
| Project/Area Number |
61570708
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Hokkaido University |
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Principal Investigator |
MASUDA Takeshi Hokkaido University School of Medicine, 医学部, 助教授 (20109424)
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| Co-Investigator(Kenkyū-buntansha) |
菅野 大己 北海道大学, 医学部附属病院, 助手 (40177770)
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| Project Period (FY) |
1986 – 1988
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| Project Status |
Completed (Fiscal Year 1988)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1988: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1987: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1986: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | Bone matrix / Non-collagenous protein / Purification of growth factor in bone / Insulin-like growth factor II / コラーゲン合成の促進 / 骨成長因子 / コラーゲン合成促進 / 骨 / 成長因子 / ヒト / 非コラーゲン蛋白質 / 熱・酸に安定 / 分子量18K |
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| Research Abstract |
Bone volume is determined by the relative rates of bone resorption and bone formation. The purpose of this study was to purify a growth factor from human bone matrix activating the proliferation of embryonic chick calvarial cells.
Materials and Methods
Cancellous bone matrix from human femoral heads were pulverized, washed to remove serum and debris, and then demineralized in 10% EDTA (pH 7.2). The growth was purified from the EDTA extract by the procedures including acetone treatment, Hw 50F colomn chromatography, sephadex G-75 superfine column chromatography, MONO-Q fast protein liquid chromatography, and reversed-phase high pressure liquid chromatorgaphy.
Results
1 In the final purification step, the growth factor was eluted as a single peak at 114 min. by the reversed-phase HPLC. The protein migrated as a single band upon SDS-PAGE.
2) Sequence of the first 30 N-terminal amino acids of the final preparation were identical to that of Insulin-like growth factor II (IGF-II).
3) The purified growth factor at 0.1 ng/ml doubled DNA synthesis, collagen synthesis and alkaline phosphatase activity compared to the control value determined in the absence of the factor.
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Report
(4 results)
Research Products
(12 results)
