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Immunohistochemical Funicular Orientation of Peripheral Nerve

Research Project

Project/Area Number 61570715
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Orthopaedic surgery
Research InstitutionShinshu University School of Medicine

Principal Investigator

SAITOH Satoru  Shinshu University School of Medicine Department of Orthopaedic Surgery(Assistant), 医学部・整形外科, 助手 (20175350)

Co-Investigator(Kenkyū-buntansha) HANDA YaSunobu  Shinshu University School of Medicine Department of Anatomy( Subprofessor), 医学部・第2解剖, 助教授 (00111790)
NAKATSUCHI Yukio  Shinshu University School of Medicine Department of Orthopaedic Surgery( Lecture, 医学部・整形外科, 講師 (80115360)
Project Period (FY) 1986 – 1987
Project Status Completed (Fiscal Year 1987)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1987: ¥200,000 (Direct Cost: ¥200,000)
Fiscal Year 1986: ¥1,900,000 (Direct Cost: ¥1,900,000)
KeywordsPeripheral Nerve / Histochemical Funicular Orientation / Immunohistochemical Method / Retrograde Changes / Acetylcholinesterase / コリンアセチルトランスフェラーゼ / 神経束縫合 / 組識化学的神経束識別法 / 免疫組識化学的神経束識別法 / アセチルコリンエステラーゼ
Research Abstract

1. Immunohistochemical funicular orientation of peripheral nerve
Immunohistochemical method was introduced into the funicular orientation of peripheral nerve. We stained the spinal roots of rat and pig with commercialy obtainable antibodies and staining kit. An anti- choline acetyltransferase antibody was used as the first antibody.
We could differentiate rat anterior and posterior spinal roots with this method. The histochemical method was, however, more advantageous than the immunohistochemical method in theanterior and posterior roots.
2. Histological and histochemical study on the retrograde changes of injured peripheral nerve
Rat sciatic nerve and gluteal muscle branch were ligated and retrograde changes were observed histologically and histochemically.
The decrease of acetylcholinesterase activity in the central stump, which has been observed by biochemical method, was observed as the decrease of teh cross-sectional area of the acetylcholinesterase positive axons. The decrease of the cross-sectional area of the axons in the central stump was confirmed statistically using micro-computer assisted image analyser.
Small myelinated fibers have remarkably increased in number in the central stump of the ligated gluteal muscle branch. But electron microscopic observation of acetylcholinesterase activity revealed that only 10 % to 20 % of the small myelinated axons in the central stump showed the enzymatic activity.
These retrograde changes progressed with the lapse of time after the ligationand were observed at the region more than 15 mm and more than 5 mm proximal to the ligated site in the ligated rat sciatic nerve and gluteal muscle branch respectively.

Report

(2 results)
  • 1987 Final Research Report Summary
  • 1986 Annual Research Report
  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] 斉藤覚,中土幸男,杉本良洋,杉浦憲治: 日本手の外科学会雑誌. 3. 59-61 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 斉藤覚,土中幸男,杉本良洋,杉浦憲治: 日本整形外科学会雑誌. 61. 132-132 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 斉藤覚,中土幸男,八木了,杉本良洋,杉浦憲治: 日本手の外科学会雑誌. 2. 595-603 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 斉藤覚,中土幸男,八木了,杉本良洋,杉浦憲治: 日本手の外科学会雑誌. 3. 70-74 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 斉藤覚: 日本整形外科学会雑誌. 61. 959-973 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 斉藤覚,中土幸男,保坂正人,杉本良洋,杉浦憲治: 日本手の外科学会雑誌. 4. 14-19 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Satoru Saitoh, Yukio Nakatsuchi, Yoshihiro Sugimot, Kenji Sugiura: "Immunohistochemical Funicular Orientation of Peripheral Nerve" J. Jpn. Soc. Surg. Hand. 3. 59-61 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Satoru Saitoh, Yukio Nakatsuchi, Ryo Yagi, Yoshihiro Sugimoto, Kenji Sugiura: "Histochemical Observation of the Retrograde Degeneration in Injured Peripheral Nerve" J. Jpn. Soc. Surg. Hand. 2. 595-603 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Satoru Saitoh, Yukio Nakatsuchi, Ryo Yagi, Yoshihiro Sugimoto, Kenji Sugiura: "Histological and Histochemical Studies of Central Stump in Peripheral Nerve Injury" J. Jpn. Soc. Surg. Hand. 3. 70-74 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Satoru Saitoh: "Histochemical Study of Retrograde Degeneration in Ligated Peripheral Nerve" J. Jpn. Orthop. Assoc.61. 959-973 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Satoru Saitoh, Yukio Nakatsuchi, Masato Hosaka, Yoshihiro Sugimoto, Kenji Sugiura: "Retrograde Changes After Peripheral Nerve Injury - Statistical and Electron Microscopic Observation of Myelinated Fibers in the Central Nerve Stump" J. Jpn. Soc. Surg. Hand. 4. 14-19 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 斉藤覚,中土幸男,杉本良洋,杉浦憲治: 日本手の外科学会誌. 第3巻1号. 59-61 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] 斉藤覚,中土幸男,杉本良洋,杉浦憲治: 日本整形外科基礎学会誌.

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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