| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1988: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1987: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1986: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Research Abstract |
The purpose of this study is to demonstrate the possibility of creating a new nutrient vascular system of the tibia with a vascular bundle. implantation and applying it as a secondary living bone graft.
Using Fisher strain F-344 adult rats, the right saphenous artery and vein were elevated as a vascular bundle, and this was implanted to the intramedullary cavity of an intact tibia (Group A), and on the periosteum of an intact tibia (Group B), and to the intramedullary cavity of an isolated tibia shaft (Group C). At several intervals after the poeration, the island vascularized tibia, nourished by the implanted saphenous vascular bundle, was elevated on the vascular pedicle and transferred to the abdominal subcutaneous space. Thereafter, microangiographic, histological, and fluorcscent labeling studies were performed.
The follwing results were obtained: (1) In Group A, survival of the bone cells was noted histologically, and on the undercalcified specimens with fluorescent labeling, the bone cells were found to be viable after the transfer to the abdominal subcutaneous space. (2) In Group B, only the bone cells in the outer layer of a cortex were revitalized after the transfer. (3) In Group C, the vascular proliferation from the implanted vessels was seen on the microangiogram. And this proliferation was more conspicuous than that of Group A. Histologically, endosteal and periosteal osteogenesis were noted. The fluorochrome uptakes were seen in some Haversian canals, but a majority of the cortex was still necrotic even at five weeks after the implantation of a vascular bundle.
The results suggest that "SECONDARY LIVING BONE GRAFT" by the implantation of a vascular bundle to the intramedullary cavity of an intact bone is realized, and can be applied clinically as a new source of vascularized bone grafts.
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