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The mechanism with damage of liver cell membranes induced by abnormal oxygen conditions

Research Project

Project/Area Number 61570736
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 麻酔学
Research InstitutionShiga University of Medical Science

Principal Investigator

YOSHIKUNI AMAKATA  Shiga University of Medical Science, 医学部, 教授 (20028567)

Co-Investigator(Kenkyū-buntansha) HIROSHI KINUGAWA  Shiga University of Medical Science, 医学部, 助手 (00177883)
Project Period (FY) 1986 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1988: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1987: ¥200,000 (Direct Cost: ¥200,000)
Fiscal Year 1986: ¥500,000 (Direct Cost: ¥500,000)
KeywordsIsolated Hepatocyte / GOT / Immunohistochemistry / Morphology Cell Organelles / ミトコンドリア / 遊離肝細胞 / 血中逸脱酵素 / ブレッブフォーメーション / 電子顕微鏡
Research Abstract

Effect of halothane on isolated rat's hepatocytes have investigated with morphologically and immunohistochemically indicated by GOT leakage. Cell damages were discussed by the decrement of microvilli using scanning electron microscope (SEM) and were checked by the changes of nucleus, mitochondria and the other cell organelles using transmission electron microscope (TEM). These were concerned with the halothane concentration.
The leakage of GOT, i.e., m-GOT plus s-GOT, was discussed by immunohistochemical staining. The decrement of s-GOT was dominantly even in the low concentration of halothane, on the other hand, m-GOT was resisted compared with s-GOT. But in the high concentration of halothane, both of them showed massive leakage of enzymes.
These results were discussed with TEM and was showed that-m-GOT existed in the cristae of mitochondria and s-GOT existed in the cytoplasm evenly.
Those investigators need to establish the route of leakage across the membrane of cell and mitochondria in further study.

Report

(4 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1987 Annual Research Report
  • 1986 Annual Research Report
  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] 絹川裕史: 麻酔と蘇生 麻酔薬代謝と肝腎障害IX. 24. 31-35 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Hiroshi Kinugawa: "Two Morphological Changes of Isolated Rat Hepatocytes induced by Halothane" Hiroshima Journal of Anesthesia (Biotransformation and Toxicity of Anesthetics IX). 24. 31-35 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 絹川裕史: 麻酔と蘇生 麻酔薬代謝と肝腎障害IX. 24. 31-35 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 絹川裕史: 麻酔と蘇生. 24. (1988)

    • Related Report
      1987 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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