| Project/Area Number |
61570785
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Obstetrics and gynecology
|
|---|
| Research Institution | Niigata University |
|---|
Principal Investigator |
TAKAHASHI Hiroaki Niigata University School of Medicine, University Hospital, 医学部附属病院, 助手 (90179515)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
TAKEUCHI Shoshichi Niigata University School of Medicine,Proffesor, 医学部, 教授 (30009914)
YOSHIYA Norio Niigata University School of Medicine,Assistant, 医学部, 助手 (90183706)
KANAZAWA Koji Niigata University School of Medicine, Associate Professor, 医学部, 助教授 (50092680)
|
|---|
| Project Period (FY) |
1986 – 1987
|
| Project Status |
Completed (Fiscal Year 1987)
|
| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1987: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1986: ¥1,400,000 (Direct Cost: ¥1,400,000)
|
|---|
| Keywords | choriocarcinoma / pulmonary metastasis / class II HLA / Metastatic factor / 形質転換 / class【II】HLA |
|---|
| Research Abstract |
Metastatic choriocarcinomas to the brain are often lethal to the patients and they are well known to come from pulmonary metastatic lesions. DNAs from metastatic GCH-1(m) cells were transfered into non-metastatic GCH-1 cells and then these transformants were inoculated subcutaneously into nude mice. Eight weeks later, metastatic tumors were yielded in lungs of all mice. LT-cell line was established from the lung tumors after injection of the transformants. GCH-1(m) DNAs were transfected into another human gestational choriocarcinoma cell line and a human ovarian carcinoma cell line. The transformants of these cell lines inoculated into nude mice, yielded lung tumors in the mice only after inoculation of transfected choriocarcinoma cells. Northern Analysis showed that GCH-1, GCH-1(m) and LT-1 transcribed class I MHC mRNA, but only GCH-1 cells transcribed class II MHC - and -chain mRNA. On the contrary, GCH-1(m) and LT-1 transcribed just H-ras mRNA without transcription of class II MHC mRNA.
|
