| Budget Amount *help |
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1987: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1986: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Research Abstract |
In the present study, concentrations of phosphatidylcholine (PC) and phosphatidylglycerol (PG) in the amniotic fluids of diabetic pregnancies were measeured and the results were compared with the severity of maternal diabetes. In cases of mild type diabetes (white categoly, A, B, C), PG levels in the amniotic fluid were decreased than those of non-diabetic controls, but in cases of severe type diabetes (white categoly D, E, F) complicating IUGR, PG levels in the amniotic fluid were increased than those of normal controls at the same gestational ages. The levels of PC in teh amniotic fluid were not affected by maternal diabetes.
The effects of hyperglycemia on the surfactant synthesis in the fetal lung were examined using animal model of diabetes, streptozotocin-induced hyperglycemia rats. The hyperglycemia caused the increased levels of phosphatidylinositol and the decreased levels of PG and cardiolipin. However, the levels of PC in the fetal lung did not altered. These results suggeste
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d that the hyperinositolemia, associated with hyperglycemia, caused the inhibition of CDP-DG: glycerol-3-phosphate phosphatidyltransferase (PG synthesis) and the enhancement of CDP-DG: inositol phosphatidyltransferase (PI synthesis). The main pathway of PC synthesis, CDP-choline:diglyceride cholinephosphotransferase was not altered by hyperglycemia.
When the levels of PC and PG in the amniotic fluid were measured serially after preterm PROM, PG levels in amniotic fluid increased sharply after they reached around 200n moles/dl irrespective to the duration of PROM or gestational ages. These results led us to speculate that the acceleration of fetal lung maturation might be related with the stress caused by premature uterine concentration after PROM.
Nicotine administration to the pregnant rabbits caused IUGR (symmetrical), the decreased fetal lung wet weight, and the decreased contents of PC and PG in lung tissue. However, PC and PG levelin the amniotic fluid did not affected by nicotine administration. These results indicated that nicotine suppressed the synthesis of PC and PG in lung tissue but enhanced the secretion into alveolar space or amniotic cavity.
The role of vitamin B_<12>, (B_<12>) and B_<12> binding protein (transcobalamin: TC) in the development of rat fetus was investigated. Maternal B_<12> deceased to 1/5-1/6 of control level after one month of B_<12> deprivation. However, B_<12> in the etal serum showed only slight decrease. The PC content of fetal lung decreased slightly but it was not statistically significant. The mechanism of maintaining B_<12> concetration offetal plasma was invest igated. Placenta was revealed to possess the extreemly effective transport system of B_<12> with high concentration of transcobalamin. In addition, maternal transcobalamin increased during pregnancy to supply B_<12> to the fetus. This increase of transcobalamin during pregnancy might be caused by progesterone, since serum level of transcobalamin increased 50-70% after one week of progesterone administration to non-pregnant rats. Less
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