The role of neuropeptides on aqueous humor dynamics:An Pharmacological and immunohistochemical analysis.
| Project/Area Number |
61570842
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Ophthalmology
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| Research Institution | Osaka University |
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Principal Investigator |
SHIMIZU Yoshiki Assistant professor of Osaka University Medical School, 医学部, 講師 (40107040)
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| Project Period (FY) |
1986 – 1987
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| Project Status |
Completed (Fiscal Year 1987)
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| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1987: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1986: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | Neuropeptide / Neuropeptide Y / substance P / Vasoactive intestinal peptide / dog ciliary body / Short circuit current / intraocular pressure / 毛様動脈 / 神経ペプチド / 短絡電流 / 免疫組織化学 |
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| Research Abstract |
1.We investigated the effect of substance P(SP) and Vasoactive intestinal peptide (VIP) on the short circuit current(SCC) of the isolated ciliary body of the dog, with consideration given to the relationship between SCC and aqueous humor formation. The isolated dog ciliary body was clamped into Ussing type chamber. SP decreased SCC when added to the aqueous side, but had no effect when added to the blood side. While VIP increased SCC when added to the aqueous side, but had no effect when added to the blood side.
2. Using immunohistochemical methods, SP, calcitonin gene-related peptide(CGRP), VIP and neuropeptide Y(NPY) were found to be localized in nerves of the dog ciliary artery. Immunoreactive nerve fibers were present in the adventitia of the ciliary artery. SP- and CGRP-immunoreactive nerves surrounded the ciliary artery sparcely, whereas VIP- and NPY-immunoreactive nerves formed a dense network around the artery. These immunoreactive nerves were not observed in the arterial muscle layer.
3. Using immunohistochemical methods, SP, CGRP, VIP and NPY were found to be localized in nerves of the dog uveal tissue. Immunoreactive nerve fibers were present widely in ciliary body, iris and choroid. These immunoreactive fibers mainly distributed around the blood vesselsof the uvea. The neuropeptides mentioned have important roles in blood flow regulation. Further physiological studies are needed, to define precise functions in blood flow control of the eye.
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Report
(2 results)
Research Products
(5 results)
