| Project/Area Number |
61570894
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Functional basic dentistry
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| Research Institution | Showa University |
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Principal Investigator |
ABE Etsuko School of Dentistry, Showa University, 歯学部, 助教授 (70119147)
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| Co-Investigator(Kenkyū-buntansha) |
TANAKA Hirofumi School of Dentistry, Showa University, 歯学部, 助手 (30146899)
MIYAURA Chisato School of Dentistry, Showa University, 歯学部, 助手 (20138382)
SUDA Tatsuo School of Dentistry, Showa University, 歯学部, 教授 (90014034)
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| Project Period (FY) |
1986 – 1987
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| Project Status |
Completed (Fiscal Year 1987)
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| Budget Amount *help |
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1987: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1986: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | Osteoclast / Osteoblast / Bone resorption / 分化誘導因子 / 骨吸収作用 / 肺胞マクロファージ / 増殖 / 分化 / DIF |
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| Research Abstract |
We have reported that protein factors separated from conditioned media of Concanavalin A-stimulated spleen cell cultures induce growth and fusion of mouse alveolar macrophages. A macrophage growth factor (MGF) was purified and, at the final step of purification on HPLC, eluted at the same position as a colony-stimulating factor (CSF), suggesting that MGF is identical with CSF. In the present study, we examined the relationship between proliferation and fusion of macrophages using purified CSF (MGF) and 1<alpha>,25-dihydroxyvitamin D_3 [1<alpha>,25(OH)_2D_3]. The latter was used in place of a macrophage fusion factor which is supposed to be contained in the same conditioned mediu, since macrophages fusion factor has not yet been isolated. Adding less than 5% unfractionated conditioned medium from Concanavalin A-stimulated spleen cells markedly induced proliferation of alveolar mcrophages without inducing fusion. In contrast, adding the same unfractionated conditioned medium at concentrations of 10% or more suppressed proliferation dose dependently, whereas it induced fusion reciprocally. Proliferationo f macrophages was similarly enhanced by adding purified CSF or retinoic acid. Fusion of macrophages was induced by 1<alpha>,25(OH)_<>D_3, but not by purified CSF or retionoic acid. Adding 1<alpha>,25-(OH)_2D_3 together with purified CSF or retinoic acid completely suppressed the increase or proliferation induced by either growth factor, whereas that treatment rather potentiated the fusion induced by 1<alpha>,25(OH)_2D_3 alone. These results indicate that the fusion and proliferation of macrophages occur in a reciprocal fashion.
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