Bioactive Amines Produced by Anaerobacteria in Periodontal Pocket

Research Project

Project/Area Number	61570908
Research Category	Grant-in-Aid for General Scientific Research (C)
Allocation Type	Single-year Grants
Research Field	Conservative dentistry
Research Institution	TOHOKU UNIVERSITY
Principal Investigator	MAITA Eikichi TOHOKU UNIVERSITY, SCHOOL of DENTISTRY, 歯学部, 助教授 (80108547)
Co-Investigator(Kenkyū-buntansha)	SASAKI Toshiaki NISHITAGA NATIONAL HOSPITAL, 西多賀病院, 医員 ENDO HIDEAKI TOHOKU UNIVERSITY, SCHOOL of DENTISTRY, 歯学部, 助手 (80168830) SHOJI Sigeru TOHOKU UNIVERSITY, SCHOOL of DENTISTRY, 歯学部, 講師 (10142986)
Project Period (FY)	1986 – 1987
Project Status	Completed (Fiscal Year 1987)
Budget Amount *help	¥1,900,000 (Direct Cost: ¥1,900,000) Fiscal Year 1987: ¥800,000 (Direct Cost: ¥800,000) Fiscal Year 1986: ¥1,100,000 (Direct Cost: ¥1,100,000)
Keywords	High performance liquid chromatography / Polyamines / Periodontal pathogenesis bacteria / 歯肉溝浸出液 / 歯周ポケット / 高速液体クロマトグラム
Research Abstract	1. Methodology of analysis of histamine and polyamines Analysis conditions for qualifying and quantifying anines were established using high performance liquid chromatography. The condition was as follows: Column:Hitachi GEL #3011C (4X250mm): Column temperature:60゜C:Mobile phase: 0.4M Mono-potasium phosphate:Elution rate:0.5ml/min Putrescine. cadaverine. histamine, spermidine, spermine were separated within twenty-two minutes, however, their peakes were accumulated a little. Minimum detecting ability was 10pmol. 2. Analysis of amines in gigival crevicular fluid Gingival crevicular fluids were collected with periopaper^R placing into the periondontal pocket of a patient suffured from heavy periodontitis. The volume of fluids was determinated with Periotron^R. Filter-paper strips were eluted by 0.1N HCIsolution. and then amines in the centrifuged supernatants were analyzed. Putrescine and spermidine were detected. the amount of each polyamine was 13.4, 11.2nmol/<micrn>l respectively. Cadaverine and spermidine were analyzed inwhole saliva 0.21, 0.08nmol/<micrn>l respectively 3. Amine formation by bacteria Fusobacterium nucleatum. Streptococcus mutans. Bacteroides gingivalis were cultured in the broth(Phytone, Yeast, extract, Hemine, Menasion) anaerobicaly None of amines, however, was detected.