| Project/Area Number |
61570911
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Conservative dentistry
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| Research Institution | OKAYAMA UNIVERSITY |
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Principal Investigator |
NOMURA Yoshio Okayama University Dental School. Assistant Professor, 歯学部, 助教授 (50107075)
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| Co-Investigator(Kenkyū-buntansha) |
SHIMIZU Hideki Okayama University Dental School. Assistant, 歯学部, 助手 (70170983)
KINOSHITA Masahiko Okayama University Dental School. Lecturer, 歯学部附属病院, 講師 (80161537)
MURAYAMA Yoji Okayama University Dental School. Professor, 歯学部, 教授 (50029972)
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| Project Period (FY) |
1986 – 1987
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| Project Status |
Completed (Fiscal Year 1987)
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| Budget Amount *help |
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1987: ¥100,000 (Direct Cost: ¥100,000)
Fiscal Year 1986: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | Fibroblast from Gingiva / Growth-promoting Factor / 細胞代謝機能 |
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| Research Abstract |
To assess the metabolism of gingival connective tissue in periodontal disease, we attempted to detect and purify a growth-promoting factor which was secreted by fibroblast themselves.
Human gingival fibroblast obtained from healthy and hyperplastic gingiva were cultured to subconfluent with Dulbeco's modified Eagle's medium containing 10% newborn calf serum (NCS), then the cells were washed with serum-free medium and exposed to serum-free medium. Following a 24 hr incubation, conditioned medium was collected. The conditioned medium containing the factor which was secreted by fibroblasts, was concentrated and used for examination.
The results were as follows:
1. Human healthy and hyperplastic gingival fibroblasts secreted a factor which stimulated DNA synthesis and collagen synthesis of gingival fibroblast into their conditioned medium. In stimulating DNA synthesis, the concentrated conditioned medium was 26 times more effective than NCS on a protein basis, and showed most potent after a 24 hr incubation.
2. Partial purification of concentrated conditioned medium by gel filtration revealed that promoting factor to DNA synthesis had a molecular size near 30K.
3. Prostaglandin A_1, D_2 and E_2 inhibit DNA synthesis of gingival fibroblast.
Growth-promoting factor derived from gingival fibroblast seems a polypeptide-like substance.
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