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The Effect of Some Serum Components on the Thiol-Dependent Collagenolytic Enzyme Isolated from Bacteroides gingivalis 381.

Research Project

Project/Area Number 61570974
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 小児・社会系歯学
Research InstitutionThe University of Tokushima

Principal Investigator

ENDO Junko  徳島大学, 歯学部, 助手 (20176796)

Co-Investigator(Kenkyū-buntansha) HINODE Daisuke  徳島大学, 歯学部, 助手 (70189801)
SATO Makoto  徳島大学, 歯学部, 助教授 (10126229)
Project Period (FY) 1986 – 1987
Project Status Completed (Fiscal Year 1987)
Budget Amount *help
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1987: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1986: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsBacteroides gingivalis / Thiol-Dependent Collagenolytic Enzyme / 血清成分
Research Abstract

成人型の辺縁性歯周炎患者の歯周ポケットより高頻度に分離されるBacteroides gingivalisの培養上清より, SH依存性のコラゲナーゼを抽出し, 精製を試みた結果, タンパク当りの比活性で115倍にまで部分精製された. 部分精製酵素に対する血清成分の影響を調べたところ, γーグロブリンのみならずアルブミンによっても阻害を受けることが判明した. 以上の結果が前年度までに得られた結果であり, 本年度は, このデータを取りまとめて報告を行なった.

Report

(3 results)
  • 1988 Annual Research Report
  • 1987 Final Research Report Summary
  • 1986 Annual Research Report
  • Research Products

    (5 results)

All Other

All Publications (5 results)

  • [Publications] 友辺芳明,遠藤順子,日野出大輔,前原玲子,永田篤司,嶋田順子,佐藤誠,中村亮: 口腔衛生学会雑誌. 37. 630-636 (1987)

    • Related Report
      1988 Annual Research Report
  • [Publications] 友辺芳明,遠藤順子,日野出大輔,前原玲子,永田篤司,嶋田順子,佐藤誠,中村亮: 口腔衛生学会雑誌. 37. 630-636 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Yoshiaki Tomobe: "Purification of Thiol-Dependent Collagenolytic Enzyme Isolated from Culture Supernatant of Bacteroides gingivalis 381 and Its Inhibition by Some Serum Components." The Journal of Dental Health. 37. 630-636 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 佐藤誠,大塚誠,遠藤順子,中村亮: 口腔衛生学会雑誌. 35. 184-185 (1985)

    • Related Report
      1986 Annual Research Report
  • [Publications] Y.Tomobe;M.Ostuka;R.Maehara;J.Endo;J.Shimada;M.Sato;R.Nakamura: Journal of Periodontal Research.

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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