| Project/Area Number |
61570975
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
小児・社会系歯学
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| Research Institution | The University of Tokushima |
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Principal Investigator |
SATO Makoto The University of Tokushima, Associate Professor, 歯学部, 助教授 (10126229)
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| Co-Investigator(Kenkyū-buntansha) |
SHIMADA Junko The University of Tokushima, Research Assistant, 歯学部, 教務職員 (10170945)
MAEHARA Reiko The University of Tokushima, Research Associate, 歯学部, 助手 (30181605)
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| Project Period (FY) |
1986 – 1987
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| Project Status |
Completed (Fiscal Year 1987)
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| Budget Amount *help |
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1987: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1986: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Periodontal Disease / Salivary IgA Antibody / Enzyme-linked Immunosorbent Assay / Bacteoides gingivalis / Actinobacillus actinomycetemcomitans |
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| Research Abstract |
The salivary IgA antibodies to the antigens extracted from Bacteroides gingivalis and Actinobacillus actinomycetemcomitans were measured with an enzyme-linked immunosorbent assay (ELISA) in 45 subjects with adult type of marginal periodontitis. The reference curve for the binding of salivary IgA to the bacterial antigens was established by testing serial dilutions of the standard saliva. The curve for each bacterial antigens was converted to the regression line using an equation for log-logit transformation with a high correlation coefficient of more than 97 per cent. Antibody activity in each saliva sample was estimated from the line for the respective antigen and was expressed as ELISA units (EU). When subjects were divided into two groups in reference to the periodontal pocket depth of 4 mm, salivary IgA levels to both B. gingivalis and A. actinomycetemcomitans were significantly higher in subjects with deeper pocketsdepth. But when they were divided in reference to the periodontal pocket depth of 5mm, the subjects with deeper pocket depth had significantly elevated IgA levels to only B. gingivalis. The result indicated that the increase of salivary IgA level to these bacteria, especially to B. gingivalis, was associated with severity of adult type of marginal periodontitis.
Moreover, antigenic characteristics of B. gingivalis strains were also investigated using gel diffusion and cell agglutination technique. These tests demonstrated that B. gingivalis strains isolated from the human mouth were serologically divided into at least two serogroups. But, in this research project, only one serogroup strain was used as the antigen. So, when determining the salivary IgA antibodies to the antigens of B. gingivalis, it seems that the different serogroups of B. gingivalis need to be used as the antigens.
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