| Project/Area Number |
61570978
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
小児・社会系歯学
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| Research Institution | Tokyo DentalCollege |
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Principal Investigator |
MAKI Yoshinobu Tokyo Dental College, School of Dentistry, Assistant Professor, 歯学部, 講師 (80125012)
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| Co-Investigator(Kenkyū-buntansha) |
OHTA Kosei Tokyo Dental College, School of Dentistry, Assistant Professor, 歯学部, 講師 (30138680)
SAZUKA Zinichiro Tokyo Dental College, School of Dentistry, Assistant Professor, 歯学部, 講師 (20158934)
TAKAESU Yoshinori Tokyo Dental College, School of Dentistry, Professor, 歯学部, 教授 (60048303)
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| Project Period (FY) |
1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1986: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | Actinomyces viscosus / Glycolytic activity / Colonization / Fluoride / pH |
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| Research Abstract |
Actinomyces species have been considered as a causative agent of both root surface caries and gingivitis. Predominancy of Actinomyces is comparable with that of Streptococci in plaque bacteria.
The purpose of this study was to investigate the effect of fluoride and pH on the glycolytic activity of Actinomyces viscosus in a chemostat as a oral model. The cultivation was run at pH 5.0-8.0 at 37゜C under nitrogen gas flow. The complex medium containing of fluoride ranged 0-500 ppm <F^-> was used. The glycolytic activity of A.viscosus was not effected by fluoride less than 50 ppm <F^-> . However, this activity became low depending on the fluoride concentrations at more than 50 ppm <F^-> . This phenomenon related to the changing pattern of the endproducts in culture medium. These results showed the change of glycolytic pathway of A.viscosus by the addition of fluoride. The change of pH also affected the glycolytic activity and its effect was cooperated with the fluoride concentration.
Subsequently, the demineralization of enamel and cementum was researched by A.viscosus in a oral model system. The level of the demineralization of cementum caused by the colonization of A.viscosus was higher than that of enamel. However, the detailed data of this study was not got yet. The colonization and demineralization by A.viscosus require further examination.
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