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Role of Ca^<2+> on c-fos expression during differentiation of leukemia cells.

Research Project

Project/Area Number 61571044
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Biological pharmacy
Research InstitutionInstitute of Medical Science, University of Tokyo

Principal Investigator

NOSE Kiyoshi  Associate Professor, Institute of Medical Science, University of Tokyo, 医科学研究所, 助教授 (70012747)

Project Period (FY) 1986 – 1987
Project Status Completed (Fiscal Year 1987)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1987: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1986: ¥1,200,000 (Direct Cost: ¥1,200,000)
Keywordsc-fos / TPA / C-kinase / 〓〓酵素 / がん遺伝子 / 発癌促進物質 / カルシウム / 転写制御 / フォルボールエスエル / 白血病細胞
Research Abstract

We analyzed mechanism of transcriptional induction of c-fos in U937 cells. Transcription of c-fos was induced by phorbol esters (TPA) and this induction required activation of protein kinase C. One of the factors which control gene expression is chromatin structure. Chromatin higher order structure was examined with induced and uninduced cells by comparing sensitivity of c-fos gene to nucleases. Four distinct sites which showed high sensitivity to DNase I were detected in a 5' upstream region of c-fos gene, but these sites and the sensitivity did not change after induction.
The induction of c-fos was found to be inhibited by inhibitors of topoisomerases, and specific cutting sites were found on 5'-upstream region of cellular c-fos gene, but these sites were not changed by inducers. From above results it was concluded that c-fos gene had active conformation even before transcriptional activation.
From competition experiments with CAT gene which had c-fos transcriptional promoter, existence of some factors was suggested which control c-fos expression negatively.

Report

(2 results)
  • 1987 Final Research Report Summary
  • 1986 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Shibanuma,M.;Kuroki,T.and Nose,K.: Eur.J.Biochem.164. 15-19 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Shibanuma,M.;Kuroki,T.and Nose,K.: Biochem.Biophys.Res.Commun.144. 1317-1323 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Itami,M.;Kuroki,T.and Nose,K.: FEBS Lett.222. 289-292 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Shibanuma,M.;Kuroki,T.and Nose,K.: J.Cell.Physiol. (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Shibanuma,M.;Kuroki,T.and Nose,K.: Oncogene. (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Shibanuma, M., Kuroki, T. and Nose, K.: "Inhibition of proto-oncogene c-fos transcription by inhibitors of protein kinase C and ion transport." Eur. J. Biochem.164. 15-19 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Shibanuma, M., Kuroki, T. and Nose, K.: "Effects of the protein kinase inhibitor H-7 and calmodulin antagonist W-7 on superoxide production in growing and resting human histiocytic leukemia cells (U937)." Biochem. Biophys. Res. Commun.144. 1317-1323 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Itami, M., Kuroki, T. and Nose, K.: "Induction of c-fos proto-oncogene by a chemotactic peptide in human peripheral granulocyte." FEBS Lett.222. 289-292 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Shibanuma, M., Kuroki, T. and Nose, K.: "Superoxide as a signal for increase in intracellular pH." J. Cell. Physiol.(1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Shibanuma, M., Kuroki, T. and Nose, K.: "Induction of DNA replication and expression of proto-oncogene c-myc and c-fos in quiescent BALB/3T3 cells by xanthine/xanthine oxidase." Oncogene. (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Namba,Masayoshi: International Journal of Cancer. 37. 419-423 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Nose,Kiyoshi: Journal of Biochemistry. 99. 1385-1391 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Sato,Chikako: Proceedings of National Academy of Sciences,U.S.A.83. 7287-7291 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Shibanuma,Motoko: European Journal of Biochemistry. (1987)

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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