| Project/Area Number |
61571097
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
応用薬理学・医療系薬学
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| Research Institution | Sapporo Medical College |
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Principal Investigator |
OHSHIKA Hideyo Professor of Pharmacology, Sapporo Medical College, 医学部, 教授 (50045358)
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| Co-Investigator(Kenkyū-buntansha) |
MIYAMOTO Atsushi Instructor of Pharmacology, Sapporo Medical College, 医学部, 助手 (50166196)
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| Project Period (FY) |
1986 – 1987
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| Project Status |
Completed (Fiscal Year 1987)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1987: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1986: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | Aging / Adrenoceptor / Signal transduction / Cholesterol / phospholipid ratio / Membrane viscosity / Cerebral cortex / Parotid gland / 心室筋 / 【α_1】-アドレナリン受容体 / 〔【^3H】〕inositol phosphates / 【K^+】放出 / ラット耳下腺 |
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| Research Abstract |
We investigated the relationship between the adrenoceptor signal transduction and the membrane composition in rat cerebral cortex, parotid gland and ventricular myocardium during aging. 1. Cerebral cortex: The density of specific [^3H]prazosin binding site reduced with advanced age, but the binding affinity for [^3H]prazosin did not. Norepinephrine (NE)-induced formation of [^3H]inositol phosphates increased with advanced age. The cholesterol content in membranes increased with advanced age; concomitantly, an increase of the ratio cholesterol/phspholipid was observed. The membrane viscosity as measured by using 1,6-diphenyl-1,3,5-hexatriene increased with advanced age.
2. Parotid gland: NE-induced of K^+ release from the slices reduced with advanced age. The density of specific [^3H]prazosin bindng site increased with advanced age, but the binding affinity for [^3H]prazosin did not. NE-induced formation of [^3H]inositol phosphates decreased with advanced age. No difference in isoprotere
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nol (ISO)-induced decrease in K^+ release and increase in cyclic AMP content were observed at any age examined. But the binding affinity for [^3H]dihydroalprenolol binding site reduced with advanced age, and the binding affinity for [^3H]dihydroalprenolol increased. The cholesterol content in membranes decreased with advanced age; concomitantly, a decrease of the ratio cholesterol/phospholipid was observed. The membrane viscosity decreased with advanced age.
3. Ventricular myocardium: The density of specific [^3H]prazosin binding site reduced with adanced age, and the binding affinity for [^3H]prazosin increased. NE-induced formation of [^3H]inositol phosphates decreased with advanced age. The cholsterol content decreased with advanced age; concomitantly, a decrease in the ratio cholesterol/phospholipid was observed. The membrane viscosity decreased with advanced age.
These results suggest that the both altered cholesterol/phospholipid ratio and/or membrane viscosity during aging may account for the changes in adrenoceptor signal transduction. Less
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