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The study of novel HLA class I gene

Research Project

Project/Area Number 61571108
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Laboratory medicine
Research InstitutionThe University of Tokyo

Principal Investigator

TAKIGUCHI Masafumi M.D.  Assistant Professor, Institute of Medical Science, University of Tokyo, 医科学研究所, 助手 (00183450)

Co-Investigator(Kenkyū-buntansha) KANO Kyoichi M.D.  Professor, Institute of Medical Science, University of Tokyo, 医科学研究所, 教授 (80152825)
ARIGA Hiroyoshi PhD  Assistant Professor, Institute of Medical Science, University of Tokyo, 医科学研究所, 助手 (20143505)
Project Period (FY) 1986 – 1987
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1987: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1986: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywordsnovel HLA class I gene / Qa gene / Qa遺伝子 / HLA抗原 / HLAC-Blank遺伝子 / 主要組織適合抗原
Research Abstract

We tried the cloning of novel HLA calss I genes by (1) the probe of 190bp mouse Q4 specific DNA or (2) the probe of HLA B7 cDNA. Several kinds of clone which hybridized with the probe (1) were cloned and all of them were transfected into mouse L cells. No HLA class I antigens on all L cell transformants was detected by the flowcytometry assay with mouse anti-HLS class I monoclonal antibodies (mAbs). On the other hand, six kinds of HLA class I genes from the library of normal peripheral blood lymphocyte (HLA-All/24, B52/52, Cw7/-) and two kinds of HLA class I genes from the library of homozygote cell line LKT-2 (HLA-A2/2. B51/51, C-/-) were isolated by the probe (2). All of them were expressed on mouse L cells. HLA-All, A24, B52, Cw7, B51 genes were determined by the absorption of corresponding alloantisera by these gene products on L cells. The remaining two genes were thougth to be C-blank gene because EchRI fragment was 7.6Kb and the binding of W6/32 mAb on these genes transfected L cells was weak.
The sequence data of C-blank gene from LKT-2 demonstrated that this gene resembled Cw1, but apparently was different from those of Cw1, Cw2 and Cw3. Only five amino acid differences (three on _1 domain and two on _2 domain) was observed between this gene and Cw1. These differences of aminoacid on _1 and _2 domain may make the novel alloantegeneicity.
The sequence experiments of another C-blank gene and an unidentified class i gene is under investigation.

Report

(3 results)
  • 1988 Final Research Report Summary
  • 1987 Annual Research Report
  • 1986 Annual Research Report
  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Ting,J.P.; Takiguchi,M.; Macchi,M.; Frelinger,A.F.: J.Neuroimmunol.14. 87-98 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Sakai,K.; Takiguchi,M.; Mori,S.; Kobori,O.; Morioka,Y.; Inoko,H.; Sekiguchi,M.; Kano,K.: J.Nat.Can.Inst.79. 923-932 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] J. P. Ting, Masafumi Takiguchi,; M. Macchi,; A. F. Frelinger: "The expression and detection of MHC class I antigens murine neuroblastoma and ependymoblastoma lines" J. Neuroimmunol.14. 87-98 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Keisuke Sakai,; Masafumi Takiguchi,; Shigeo Mori,; Oichiro Kobori,; Yasuhiko Morioka,; Hidetoshi Inoko,; Morimasa Sekiguchi,; Kyoichi Kano: "Expression and Function of Class II Antigens on Gastric Carcinoma Cells and Gastric Epithelia: Defferential Expression of DR. DQ, and DP Antigens" J. Nat. Can. Inst.79. 923-932 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Hideyuki Hayashi,; Peter D. Ennis,; Hiroyoshi Ariga,; Russell D. Salter,; Peter Parham,; Kyoichi Kano,; Masafumi Takiguchi,: "HLA-B51 differ from HLA-Bw52 by two amino acids in the helical region of domain"

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Masafumi Takiguchi,; Hideyuki Hayashi,; Isao Nishimura; Kyo ichi Kano: "The cloning and Expression of gene encoding serologically undetected HLA C locus antigens"

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ting, J.P., Takiguchi, M., Macchi, M., and Frelinger, A.F.: J. Neuroimmunol.14. 87-98 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Sakai, K., Takiguchi, M., Mori, S., Kobori, O., Morioka, Y., Inoko, H., Sekiguchi, M., and Kano, K.: J. Nat. Can. Inst.79. 923-932 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] J.P.Ting: J.Neuroimmunol.(1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] H.Ariga: Nucleic Acids Res.14. 9457-9470 (1986)

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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