Genetic monitoring of inbred mice by use of anti-Ly monoclional antibodies
| Project/Area Number |
61580042
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Laboratory animal science
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| Research Institution | Tokai University |
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Principal Investigator |
TADA Nobuhiko Tokai University School of Medicine, 医学部, 講師 (80167494)
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| Co-Investigator(Kenkyū-buntansha) |
TAMAOKI Norikazu Tokai University School of Medicine, 医学部, 教授 (50055860)
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| Project Period (FY) |
1986 – 1987
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| Project Status |
Completed (Fiscal Year 1987)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1987: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1986: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | Mouse / Immunogenetics / Genetic monitoring / リンパ球抗原 / モノクローナル抗体 |
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| Research Abstract |
The mouse lymphocyte alloantigen system, which is named Ly-28, was defined using monoclonal antibodies. Spleen cells of an AKR mouse which was immunized with BALBRV4 tumor cells were fused with BALB/c-derived myeloma cells SP2/2 by cell hybridization. According to the assays of separated T and B cells, as well as blast cells, the Ly-28.2 antigen is carried on both cell lineages.
The genes controlling Lyb-2 and Ly-19 alloantigens are closely linked on mouse chromosomo 4. We added one more gene, i.e.,Ly-32, defined by the use of monoclonal antibodies, to this Lyb-2-Ly-19 region. Two monoclonal antibodies which detect the new specificity Ly-32.2, were established by the same ce fusion described above.The lymphoid tissue distribution of the Ly-32 alloantigen suggestts rather broad expression of this alloantigen on the lymphoid cells including T cells, B cells, and stem cells.
These two alloantibodies will be a useful tool foe the stury of genetic monitoring of inbred mice.
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Report
(2 results)
Research Products
(11 results)
