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Analysis of replication of tobacco mosaic virus by means of an in vitro transcription system

Research Project

Project/Area Number 61580129
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 物質生物化学
Research InstitutionUniversity of Tokyo

Principal Investigator

MESHI Tetsuo  University of Tokyo, Faculty of Science, 理学部, 助手 (40157813)

Co-Investigator(Kenkyū-buntansha) OKADA Yoshimi  University of Tokyo, Faculty of Science, 理学部, 教授 (30011703)
Project Period (FY) 1986 – 1987
Project Status Completed (Fiscal Year 1987)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1987: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1986: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsTobacco Mosaic Virus / Replication / 試験管内合成系
Research Abstract

The genome of tobacco mosaic virus (TMV) is a single-stranded, messenger-sense RNA composed of about 6,400 uncleotides. TMV RNA encodes 4 proteins: 130k, 180k, 30k and coat proteins. The 130k and 180k proteins are translated from the same initiatin codon and the latter is synthesized by read-through of the amber termination codon of the 130k protein gene. The purpose of this research is to lead to a bettwe understanding of TMV replication. First, we estabilished an in vitro transcription system that provides infectious TMV RNAs from cloned full-length cDNAs. By using this system, we constructed verious mutant TMV RNAs and analyzed their characters. Results showe that both the 130k and 180k proteins are involved in replication, only the 180k protein is the indispensable protein for replication, there are no cis-acting elements in the 30k and coat protein gene sequences, these two proteins are dispensable for replication, and that the relationship between the putative replicase (the 130k and 180k proteins) and 3' non-coding sequence is not so stringent. Besides these results, it was reveald that the 130k and 180k proteins are strongly localized in a cytplasmic structure formed specific to infection. In the course of the research, we developed an improved electroporation method by which tobacco protoplasts can be efficiently infected with a small amount of TMV RNA.

Report

(2 results)
  • 1987 Final Research Report Summary
  • 1986 Annual Research Report
  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] Tetsuo Meshi: Proc.Natl.Acad.Sci.USA. 83. 5043-5047 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Masayuki Ishikawa: Nucl.Acids Res.14. 8291-8305 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Nobuhiko Takamatsu: EMBO J.6. 307-311 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Yuichiro Watanabe: FEBS Lett.219. 65-69 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Tetsuo Meshi: EMBO J.6. 2557-2563 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Tetsuichiro Saito: Virology. 160. 477-481 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Tetsuo Meshi: "In vitro transcription of infectious RNAs from full-length cDNAs of tobacco mosaic virus" Proc. Natl. Acad. Sci. USA. 83. 5043-5047 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Masayuki Ishikawa: "In vitro mutagenesis of the putative replicase genes of tobacco mosai virus" Nucl. Acids Res.14. 8291-8305 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Nobuhiko Takamatsu: "Expression of bacterial chloramphenical acetyltransferase gene in tobacco plants mediated by TMV-RNA" EMBO J.6. 307-311 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Yuichiro Watanabe: "Infection of tobacco protoplasts with in vitro transcribed tobacco mosaic virus RNA using an improved electroporation method" FEBS Lett.219. 65-69 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Tetsuo Meshi: "Function of the 30kd protein of tobacco mosaic virus: involvement in cell-to-cell movement and dispensability for replication" EMBO J.6. 2557-2563 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] T.Meshi: Proc.Natl.Acad.Sci.USA. 83. 5043-5047 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] M.Ishikawa: Nucl.Acids Res.14. 8291-8305 (1986)

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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