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Mechanisms of radiation-induced interphase death of thymocytes expressed as apoptosis

Research Project

Project/Area Number 61580184
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 放射線5生物学
Research InstitutionNational Institute of Radiological Sciences

Principal Investigator

OHYAMA Harumi  NIRS(Sinior researcher), 障害臨床研究部, 主任研究官 (70160645)

Co-Investigator(Kenkyū-buntansha) YAMADA Takeshi  NIRS(Sinior researcher), 生物研究部, 主任研究官 (30166714)
Project Period (FY) 1986 – 1987
Project Status Completed (Fiscal Year 1987)
Budget Amount *help
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1987: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1986: ¥1,000,000 (Direct Cost: ¥1,000,000)
KeywordsRadiation-induced interphase death / Thymocytes / Apoptosis / Programmed cell death / Protein synthesis / Actinomycin D / アクチノマイシンD / 胸腺細胞 / タンパク質変化
Research Abstract

Thymocytes are highly radiosensitive and show interphase death within a few hours after low doses of irradiation. recently,we obtained evidence to indicate that the radiation-induced interphse death of thymocytes is a king of programmed death named"apoptosis". In the present study,the mechanisms underlying the cell death were investigated.
Rat thymocytes irradiated in vitro with 1 KR X-rays underwent interphase death,judged by erythrosin B staining,accompanying discrete cell size reduction and DNA degradation. The cell death as well as these changes were completely blocked by inhibitors of RNA and protein synthesis such as actinomycin D and cycloheximide.These results suggest strongly that the cell death is not a passive event,but an active process requiring the programmed synthesis of specific RNA and proteins. After 10 KR irradiation, however, gradual swelling of cells, oen of the characteristic changes of necrosis,was observed and cell death could no more be prevented by cyckoheximide.It is possible,therefore,that some process of protein synthesis reauired to develop apotosis was damaged by the high doses irradiation.As a result cells die not by apoptosis,but by necrosis after massive doses irradiation.For detection of proteins which might perticipate in paoptosis,two demensional ge%i elcrophoresis of the proteins of the viable and dead cells separated by Percoll density gradient was conducted. In comparison with the proteins of viable cells we could detect specific appearance of 27 kDa protein and disapearance of 29 kDa protein in the dead cells.Investigations concerning these proteins are now in progress.

Report

(2 results)
  • 1987 Final Research Report Summary
  • 1986 Annual Research Report
  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Ohyama,Harumi: Proc.XIth int.Congr.Electron Microscopy(Kyoto). 2853-2854 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Ohyama,Harumi: Proc.Int.Congr.Radiat.Res.(Edinburgh). 1. 167 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Yamada,Takeshi: International Journal of Radiation Biology. 53. 65-75 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 山田武: 第3回東関東肝臓集談会講演集,細胞死特集. 7-23 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 大山ハルミ: 医学のあゆみ. 144. 726 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Harummi Ohyama: "Changes in surface morphology of rat thymocytes accompanying radiation-induced interphase death" Proc.XIth Int.Congr.Electron Microscopy (Kyoto). 2853-2854 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Harumi Ohama: "Involvement of RNA and protein synthesis in rat thymocyte interphase death." Proc.8th International Congress of Radiation Research. 1. 165 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Takeshi Yamada: "Radiation-induced interphase death of rat thymocyte is internally programmed." International Journal of Radiation Bioloby. 53. 65-75 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] H.Ohyama: Proc.【X】th Congress on Electron Microscopy. 2853 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] T.Yamada: Int.J.Radiat.Biol.

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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