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ANALYSIS OF THE FOS GENE FUNCTION IN THE CELL CYCLE.

Research Project

Project/Area Number 61580218
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 分子遺伝学・分子生理学
Research InstitutionFACULTY OF SCIENCE, UNIVERSITY OF TOKYO

Principal Investigator

IBA Hideo  FACULTY OF SCIENCE, UNIVERSITY OF TOKYO, 理学部, 助教授 (60111449)

Co-Investigator(Kenkyū-buntansha) OKADA Yoshimi  FACULTY OF SCIENCE, UNIVERSITY OF TOKYO, 理学部, 教授 (30011703)
Project Period (FY) 1986 – 1987
Project Status Completed (Fiscal Year 1987)
Budget Amount *help
¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1987: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1986: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordsCell cycle / Fibroblast / Anti-sense RNA / v-fos gene / c-fos gene / 細胞周期 / 線維芽細胞 / マイクロインジェクション / 増殖因子
Research Abstract

In this research project, we intended to analyze the function of the c-fos gene that is transiently expressed upon external growth stimulations. For this purpose, we have tried to specifically inhibit the c-fos gene expression using anti-sense RNA or anti-fos antibody. Following materials or cell systems have been prepared for the project.
1. Isolation of v-fos transformed rat cell lines.
2. Preparation of a large amount (100 ug) og anti-sense RNA for v-or c-fos gene using SP6 RNA polymerase reaction in vitro.
3. Construction of retrovirus vectors which encode v-fos or c-fos (the vector can express v- or c-fos gene in CEF quite efficiently)
4. Construction of retrovirus vectors which express v-fos or c-fos anti-sensa RNA in CEF.
5. Cloning and nucleotide sequence analysis of chicken c-fos gene.
6. Preparation of anti-fos antibody which can immunoprecipitate v-fos, human, chicken and mouse c-fos gene products.
We have tried to screen the anti-sense RNA which has most inhibitory effect on v-fos or c-fos gene expression, by microinjection of antisense RNA or anti-sense RNA producing virus vectors. At present, we have find no effective anti-sense RNA and some residual expression of fos was always detectable. We are now trying to microinject anti-fos antibody into the cells for the specific and complete inhibition of the function of c-fos gene product.

Report

(2 results)
  • 1987 Final Research Report Summary
  • 1986 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] H.Nakano: J.Antibiotics. 40. 706-708 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] H,Iba: Oncogne Research. in press. (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] K,T,Fujiwara: J,Virol. 61. 4012-4018 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] J,B,Levy: Proc.Natl.Acad.Sci.USA. 83. 4228-4232 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] J,Kato: Mol.Cell.Biol.6. 4155-4160 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] H. Nakano, E. Kobayshi, T. Takahashi, T. Tamaki, Y. Kuzuu, and H. Iba: "Staurosporine infibits tyrosine-specific protein kinase activity of Rous sarcoma virus transforming protein p60." J. Antibiotics. 40. 706-708 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] H. Iba, Y. Shindo, H. H. Nishina and T. Yoshida: "Transforming potential of the v-fos and c-fos genes carried by avian retrovirus vectors." Oncogene Research.in press. (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] K. T. Fujiwara, K. Ashida, H. Nishina, H. Iba, N. MIyajima, M. Nishizawa, & S. Kawai: "The Chicken c-fos gene; c10ning and nucleotide sequence analysis" J. Virol. 61. 4012-4018 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] J. B. Levy et al.: "activation of the transforming poteintial of p60^<c-src> by a single amino acid change." Proc. Natl. Acad. Sci USA. 83. 4228-4232 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] J. Kato: "Amino acid substitutions sufficient to convert the nontransforming p60^<c-src> protein to a transforming protein." Mol. Cell. Biol.6. 4155-41609 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] J.B.Levy: Proc.Natl.Acad.Sci.U.S.A.83. 4228-4232 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] 加藤順也: Mol.Cell.Biology. 6. (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] R.Jove: J.Virsl.60. 840-848 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] R.Jove: J.Virsl.60. 849-857 (1986)

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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