| Project/Area Number |
61850102
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| Research Category |
Grant-in-Aid for Developmental Scientific Research
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| Allocation Type | Single-year Grants |
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| Research Field |
都市工学・衛生工学
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| Research Institution | Kyoto University |
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Principal Investigator |
MATSUI Saburo Kyoto Univ, Fac, of Eng. Prof., 工学部, 教授 (90092808)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAMOTO Ryoko Kanazawa Univ. Fac. of Eng. Research Assoc., 工学部, 助手 (40159223)
YAMADA Harumi Kyoto Univ. Fac. of Eng. Research Assoc., 工学部, 助手 (40089123)
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| Project Period (FY) |
1986 – 1987
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| Project Status |
Completed (Fiscal Year 1987)
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| Budget Amount *help |
¥4,600,000 (Direct Cost: ¥4,600,000)
Fiscal Year 1987: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1986: ¥4,100,000 (Direct Cost: ¥4,100,000)
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| Keywords | Bacillus subtilis / Rec-assay / Environmental Mutagens / 環境変異原 / 自動培養測定装置 |
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| Research Abstract |
An automatic instrument( Rec-assay biophotorecoder) was developed ,which can detect the growth of Bacillus subtilis in 36 Monod tubes by measuring automatically the turbidity with photometers under conditions of constant temperature and acration of shaking. Moreover, the results of detection of the growth can be introduced into a new analysis program of rec-assay based upon Probit theory and Target theory. The operation of the automatic instrument is controlled by a NEC-PC 9800 personal computer and the results are also analyzed by the new soft program developed by us. Thus a complete system of the bacteris growht, measurement and analysis is now available. The Bacillus subtilis rec-assay is a very usuful tool to detect environmental mutagens. The original agar plate and streak method developed by Dr. Kada had a limitation of application to evaluate the assay results in terms of quantitative analysis. However, the development of the liquid rec-assay by Matsui enabled the rec-assay more usuful tool. In addition, this Automatic instrument can make the rec-assay more easy and applicable method to detect mutagens by reducing labour works, obraining stable results with the statistical analysis and producing comparable results among different laboratories.
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