| Project/Area Number |
61870003
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| Research Category |
Grant-in-Aid for Developmental Scientific Research
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| Allocation Type | Single-year Grants |
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| Research Field |
神経解剖学
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| Research Institution | Osaka Univerity |
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Principal Investigator |
SHIO@TANI Yane osaka university Nedical School, Institute of Higher Nervous Activity, 医学部, 教授 (60028347)
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| Co-Investigator(Kenkyū-buntansha) |
SHIOSAKA Sadao Osaka University Medical School, Institute of Higher Nervous Activity, 医学部, 助教授 (90127233)
遠山 正弥 (遠山 正彌) 大阪大学, 医学部, 教授 (40028593)
OSAKA University Medical
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| Project Period (FY) |
1986 – 1987
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| Project Status |
Completed (Fiscal Year 1987)
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| Budget Amount *help |
¥6,500,000 (Direct Cost: ¥6,500,000)
Fiscal Year 1987: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1986: ¥5,000,000 (Direct Cost: ¥5,000,000)
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| Keywords | in situ hybridization histochemistry / preproenkephalin / preprotachykinin / mRNA / in situハイブリダイゼイション / プレプロエンケファリンA / プレプロタキキニンA / 神経活性物質 / オートラジオグラフィー |
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| Research Abstract |
In situ hybrization histochemistry is one of the new techniques based on molecuar biology and bene engeneering. This enables the visualization of specific mRNAs in tissues as radioisotope or other chemical signals. The purpose of this project is accomplishment of stable, highly sensitive, and convenient technique of the in sity hybridization histochemistry.
Experiments were based upon four caterfories. The dirst is the fixation condition to fix a mRNA on the tissue section of brain. The secons is the sensiticity and resolution of isotopes used and non-isoropic markers. The third is labeling peorcedures for the tracer into nucleotide probes. And, the last is chaecking of hybridization, washing for hybridization process and exposure conditions for autoradiography. We selected the best conditions on the each check points desctibved above, and prepared the protocol for high sensitive and high resolution technique of in situ hybridization histochmistry.
cncerning to non-isotopic in situ hybridization,we are eager to accoomlish, because of it's stafety and convenience. Now, we are triing the application of phorobiotin system for labeling of probes and enzymatic of flurescence bisualization system fro observation of probes.
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