| Project/Area Number |
61870005
|
|---|
| Research Category |
Grant-in-Aid for Developmental Scientific Research
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
General physiology
|
|---|
| Research Institution | Shinshu University School of Medicine |
|---|
Principal Investigator |
OHHASHI Toshio Shinshu University School of Mericine professor, 医学部, 教授 (80020832)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KAWAI Yasuaki Shinshu University School of Medicine Lecturer, 医学部, 講師 (70143972)
SAKAGUCHI Masao Nagano nAtional College of Technology Professor, 機械科, 教授 (70043031)
|
|---|
| Project Period (FY) |
1986 – 1987
|
| Project Status |
Completed (Fiscal Year 1987)
|
| Budget Amount *help |
¥6,800,000 (Direct Cost: ¥6,800,000)
Fiscal Year 1987: ¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 1986: ¥4,000,000 (Direct Cost: ¥4,000,000)
|
|---|
| Keywords | image senseo / area image sensor / X-ray sensitive image sensor / window single / contrast medium / outer diameter / inner diameter / wall thickness / small arteries and veins / small lymph vessels / microcirculation / new method / constriction / relaxation / 薬理学的解析法 / 不可視物体 / 血管内径 / イヌ / 腎動脈 |
|---|
| Research Abstract |
A camete-type diameter gauge was constructed and used in physiological experiments. The distinctive features of the design and use of the gauge may be summatized as follows. Installation of an image sensor in the focal plane of a single-lines reflex camera equipped with a set of extension tubes which makes it possible to measure outer diameters of blood and lymph vessels as small as 1000 <micrn>m. Using the gauge, we can distinguish a diameter change of about 12 <micrn>m. Simultaneous measutements of the diameters of two differnet vessels can be made using two window signals generated independently at desired moments for appropriate periods within the video sacna. The gauge is also able to observe network patterns of small blood and lymph vessels in mictocirculation and measure outer diameters of two different vessels by exchanging the image sensor to an area sensor. We. have designed and constructed a new device to measure an outer and inner diameter and wall thickness of samll blood vessels in microcirculation, utilizing a high resolving power and stability of the X-ray sensitive imabe sensor. The outer and inner diameter of small blood vessls were measured by using of photoand X ray-electric conversion system of the X-ray image sensor with a contrast medium which were perfused in blood stream, respectively. The wall thickness were also measured electrically to calculate the balance between the outer and inner diameter.
Longitudinal strips isolated from canine distributing muscular arteries were relaxed by noradrenaline and contracted by papaverine. This paradoxical relaxation and contraction may be due to a helical and chrcular arrangement of smooth muscle in the wall which is incompressible. By using the paradoxical phenomena, we developed a new experimental experimental method by which the response of vascular smooth muscles in very small blood vessels (arterioles) could be examined.
|
