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Construction of apparatus measuring micro-potential field around excitable tissue

Research Project

Project/Area Number 61870007
Research Category

Grant-in-Aid for Developmental Scientific Research

Allocation TypeSingle-year Grants
Research Field General physiology
Research InstitutionNagoya University, Medical School

Principal Investigator

TOMITA Tadao  Nagoya University, Medical School, 医学部, 教授 (50078763)

Co-Investigator(Kenkyū-buntansha) TOKUNO Hiroyuki  Nagoya University, Medical School, 医学部, 助手 (60155520)
Project Period (FY) 1986 – 1987
Project Status Completed (Fiscal Year 1987)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1987: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1986: ¥2,400,000 (Direct Cost: ¥2,400,000)
Keywordsfield potential of biological tissue / measutement of micro-potential field / vibrating electrode / 生体電気発生
Research Abstract

Gastrointestinal smooth muscles produce a spontaneous electrical activity (slow wave) which is responsible for automaticity. In order to investigate the initiation and propagation of the slow wave, a device was constructed to detect a very weak potential field caused by slow waves around the smooth muscle, using a vebrating electrode. When the electrode vibrates, the potential difference within vibration distance is modulated at a frequency of vibration. This modulated signal can be amplified by a lock-in amplifier with a high signalnoise ration. A piezoelectric vibrator provided by the Tohoku Kinzoku Kogyo and a lock-in amplifier (type 5600) made by NF Electronic Instrument gave satisfactory results. Glass microelectrodes were used both as a vibrating probe and an indifferent electrode. The electrodes had tip diameter of 2-5 <micrn>m and resistance of 1-5 m<omega> when filled with physiological solution. Vibration frequency was usually set at around 20 Hz and its amplitude at 10-20 <micrn>m. With this recording system, slow potential changes of about 5 <micrn>V could be recorded above the circular muscle layer of guinea-pig stomach. This potential was considered to be due to the slow ware, because it disappeared when vibration was stopped and when the electrode was moved away from the preparation. This method will be useful for a continuous recording of electrical activity. Since the fibre arrangement is very complicated, however, it is necessary to use a very fine preparation for quantitative analysis of the recorded potential field in relation to the site of generation or propagation of slow wave within the tissue.

Report

(2 results)
  • 1987 Final Research Report Summary
  • 1986 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Takai,A. and Tomita,T.: Journal of Physiology. 381. 65-75 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Ashoori,F.,Hidaka,H. and Tomita,T.: British Journal of Pharmacology. 87. 15-22 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Honda,K.,Satake,T.,Takagi,K. and Tomita,T.: British Journal of Pharmacology. 87. 665-671 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Chihara,S. and Tomita,T.: British Journal of Pharmacology. 91. 789-798 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Tokuno,H. and Tomita,T.: European Journal of Pharmacology. 141. 131-133 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Takai, A. and Tomita, T.: "Glycolysis and oxidative phosphorylation during activation of the sodium pump in the taenia from quinea-pig caecum." Journal of Physiology. 381. 65-75 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Ashoori, F., Hidaka, H. and Tomita, T.: "Carbachol-induced contraction in the circular muscle of guinea-pig stomach in calcium-free solution." British Journal of Pharmacology. 87. 15-22 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Honda, K., Satake, T., Takagi, K. and Tomita, T.: "Effects of relaxants on electrical and mechanical activities in the guinea-pig tracheal muscle." British Journal of Pharmacology. 87. 665-671 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Chihara, S. and Tomita, T.: "Mechanical and electrical responses to a-adrenoceptor activation in the circular muscle of guinea-pig stomach." British Journal of Pharmacology. 91. 789-798 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Tokuno, H. and Tomita, T.: "Collagenase eliminates the electrical responses of smooth muscle to catecholamines." European Journal of Pharmacology. 141. 131-133 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 中山晋介: 日本生理誌. 48. 58 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Chihara,S.;Tomita,T.: Br.J.Pharmac.

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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