| Project/Area Number |
61870015
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| Research Category |
Grant-in-Aid for Developmental Scientific Research
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| Allocation Type | Single-year Grants |
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| Research Field |
General medical chemistry
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| Research Institution | KOBE UNIVERSITY |
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Principal Investigator |
NISHIZUKA Yasutomi KOBE UNIVERSITY, SCHOOL OF MEDICINE, PROFESSOR, 医学部, 教授 (10025546)
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| Co-Investigator(Kenkyū-buntansha) |
OGITA Kouji KOBE UNIVERSITY, SCHOOL OF MEDICINE, ASSISTANT, 医学部, 助手 (60204103)
KIKKAWA Ushio KOBE UNIVERSITY, SCHOOL OF MEDICINE, LECTURER, 医学部, 講師 (40150354)
KISHIMOTO Akira KOBE UNIVERSITY, SCHOOL OF MEDICINE, LECTURER, 医学部, 講師 (60127363)
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| Project Period (FY) |
1986 – 1988
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| Project Status |
Completed (Fiscal Year 1988)
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| Budget Amount *help |
¥16,000,000 (Direct Cost: ¥16,000,000)
Fiscal Year 1988: ¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 1987: ¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 1986: ¥8,000,000 (Direct Cost: ¥8,000,000)
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| Keywords | PROTEIN KINASE C / SPECIFIC ANTIBODY / MOLECULAR CLONING / BIOLOGICAL ACTIVE SUBSTANCES / 受容伝達機構 / プロテインキナーゼ / モノクロナール抗体 |
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| Research Abstract |
The physiological importance of protein kinase C (PKC) activation is widely appreciated and well documented. It is now clear that there is more than noe species of PKC molecule, and several discrete subspecies have been defined. These proteins are derived from both multiple genes and from alternative splicing of a single RNA transcript, yet possess a primary structure containing conserved structural motifs with a high degree of sequence homology. The enzyme subspecies purified from tissues show subtle differences in their mode of activation, sensitivity of ca^<2+>, and catalytic activity toward endogenous substrates. In the brain tissues, for example, at least seven subspecies can be distinguished, one of which is expressed only in the central nervous tiuuse. Biochemical and immunocytochemical studies with subspecies-specific antibodies suggest that the PKC subspecies may be differently located in particular cell types and at limited intracellular locations. Many cell types so far examined express more than one subspecies in variable ratios, and their intracellular distribution may depent on the state of activation of the cells. Although, at present, there is little evidence to discuss specific functions of each PKC subspecies, the members of the enzyme family may have distinct roles in the processing and modulation of a variety of physiological and pathological cellular responses.
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