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Development of an ELISA Kit to detect ST produced by enterotoxigenic Esherichia coli by using chemically synthesized antigens.

Research Project

Project/Area Number 61870022
Research Category

Grant-in-Aid for Developmental Scientific Research

Allocation TypeSingle-year Grants
Research Field 細菌学
Research InstitutionThe Institute of Medical Science, The University of Tokyo

Principal Investigator

TAKEDA Yoshifumi  The Institute of Medical Science, The University of Tokyo, 医科学研究所, 教授 (30029772)

Co-Investigator(Kenkyū-buntansha) TAKEDA Tae  National Children's Medical Research Center, 小児医療研究センター, 部長 (40112065)
SHIMONISHI Yasutsugu  Institute of Protein Research, Osaka University, 蛋白質研究所, 教授 (00029951)
Project Period (FY) 1986 – 1987
Project Status Completed (Fiscal Year 1987)
Budget Amount *help
¥14,500,000 (Direct Cost: ¥14,500,000)
Fiscal Year 1987: ¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 1986: ¥10,500,000 (Direct Cost: ¥10,500,000)
KeywordsChemically synthesized ST / enterotoxigenic Escherichia coli / competitive ELISA / ELISA
Research Abstract

1) A competitive ELISA system to detect ST produced by enterotoxigenic Escherichia coli was developed. A chemically synthesized STh analogue that was conjugated with borine serum albumin was used as the solid phase. As the antibody, two kinds. of monoclonal antibodies each of which recognized different epitopes of STh were used and they were conjugated with horse raddish peroxidase.
2) The developed competitive ELISA sucessfully detected ST produced by about 200 standard E. coli strains and about 1700 strains of E. coli isolated from patients of traveller's diarrhoea.
3) Mode of disulfide linkages of ST was determined.
4) The developed ELISA was applied to study the structure-activity relationship of ST

Report

(2 results)
  • 1987 Final Research Report Summary
  • 1986 Annual Research Report
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] Y.Takeda;T.Takeda;T.Miwatani;S.Aimoto;H.Ikemura;H.Watanabe;S.Yoshimura;M.Miki;& Y.Shimonishi: Advances in Research on Cholera and Related Diarrheas. 3. 143-151 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Y.Shimonishi;Y.Hidaka;M.Koizumi;M.Hane;S.Aimoto;T.Takeda;T.Miwatani & Y.Takeda: FEBS Letters. 215. 165-170 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] S.Yoshimura;Y.Hidaka;S.Aimoto;Y.Shimonishi;T.Takeda;T.Miwatani & Y.Takeda: Bull.Chem.Soc.Jpn.60. 2481-2489 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] S.Yamasaki;Y.Hidaka;H.Ito;Y.Takeda;& Y.Shimonishi: Bull.Chem.Soc.Jpn.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Y.Hidaka;H.Kubota;S.Yoshimura;H.Ito;Y.Takeda;& Y.Shimonishi: Bull.Chem.Soc.Jpn.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Y.Takeda, T.Takeda,T.Miwatani, S.Aimoto, H.IKemura, H.Watanabe, S.Yoshimura, M.Miki and Y.Shimonishi: "Chemical synthesis of Escherichia coli heat-stable enterotoxins (STh and STp) and their biological and immunological properties." Advances in Research on Cholera and Related Diarrhas. 3. 143-151 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Y.Shimonishi, Y.Hidaka, M.Koizumi, M.Hane, S.Aimoto, T.Takeda, T.Miwatani and Y.Takeda: "Mode of disulfide bond formation of a heat-stable enterotoxin (STh) produced by a human strain of enterotoxigenic Escherichia" FEBvS Letters. 215. 165-170 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] S.Yoshimura, Y. Hidaka, S.Aimoto, Y.Shimonishi, T.Takeda, T.Miwatani and Y.Takeda: "Structure-activity relationship of a heat-stable enterotoxin produced by Yersinia enterocolitica" Bull. Chem. Soc. Jpn.60. 2481-2489 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] S.Yamasaki, Y.Hidaka, H<Ito, Y.Takeda and Y. Shimonishi: "Structural requirements for the spatial sturucture and toxicity of heat-stable enterotoxin (STh) of enterotoxigenic Escherichia coli" Bull. Chem. Soc. Jpn.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Y.Hidaka, H.Bubota,S. Yoshimura, H.Ito, Y. Takeda and Y.Shimonishi: "Disulfide linkages in a heat-stable enterotoxin (STp) produced by a porcine strain of enterotoxigenic Escherichia coli" Bull. Chem. Soc. Jpn.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] S.Yoshimura;H.Ikemura;H.Watanabe;S.Aimoto;Y.Shimonishi;S.Hara;T.Takeda;T.Miwatani;Y.Takeda: FEBS Letters. 138. 138-142 (1985)

    • Related Report
      1986 Annual Research Report
  • [Publications] T.Takao;N.Tominaga;S.Yoshimura;Y.Shimonishi;S.Hara;T.Inoue;A.Miyama: European Journal of Biochemistry. 152. 199-206 (1985)

    • Related Report
      1986 Annual Research Report
  • [Publications] S.Yoshimura;Y.Shimonishi: Bull.Chem.Soc.Japan. 58. 2805-2811 (1985)

    • Related Report
      1986 Annual Research Report
  • [Publications] T.Takao;Y.Shimonishi;M.Kobayashi;O.Nishimura;M.Arita;T.Takeda;T.Honda;T.Miwatani: FEBS Letters. 193. 250-254 (1985)

    • Related Report
      1986 Annual Research Report
  • [Publications] S.Yoshimura;T.Takao;Y.Shimonishi;S.Hara;M.Arita;T.Takeda;H.Imaishi;T.Honda;T.Miwatani: Biopolymers. 25. S69-S83 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Y.Takeda;T.Takeda;T.Miwatani;S.Aimoto;H.Ikemura;H.Watanabe;S.Yoshimura;M.Miki;Y.Shimonishi: Advances in Research on Cholera and Related Diarrheas. 3. 143-151 (1986)

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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