Development of an ELISA Kit to detect ST produced by enterotoxigenic Esherichia coli by using chemically synthesized antigens.
| Project/Area Number |
61870022
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| Research Category |
Grant-in-Aid for Developmental Scientific Research
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| Allocation Type | Single-year Grants |
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| Research Field |
細菌学
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| Research Institution | The Institute of Medical Science, The University of Tokyo |
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Principal Investigator |
TAKEDA Yoshifumi The Institute of Medical Science, The University of Tokyo, 医科学研究所, 教授 (30029772)
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| Co-Investigator(Kenkyū-buntansha) |
TAKEDA Tae National Children's Medical Research Center, 小児医療研究センター, 部長 (40112065)
SHIMONISHI Yasutsugu Institute of Protein Research, Osaka University, 蛋白質研究所, 教授 (00029951)
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| Project Period (FY) |
1986 – 1987
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| Project Status |
Completed (Fiscal Year 1987)
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| Budget Amount *help |
¥14,500,000 (Direct Cost: ¥14,500,000)
Fiscal Year 1987: ¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 1986: ¥10,500,000 (Direct Cost: ¥10,500,000)
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| Keywords | Chemically synthesized ST / enterotoxigenic Escherichia coli / competitive ELISA / ELISA |
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| Research Abstract |
1) A competitive ELISA system to detect ST produced by enterotoxigenic Escherichia coli was developed. A chemically synthesized STh analogue that was conjugated with borine serum albumin was used as the solid phase. As the antibody, two kinds. of monoclonal antibodies each of which recognized different epitopes of STh were used and they were conjugated with horse raddish peroxidase.
2) The developed competitive ELISA sucessfully detected ST produced by about 200 standard E. coli strains and about 1700 strains of E. coli isolated from patients of traveller's diarrhoea.
3) Mode of disulfide linkages of ST was determined.
4) The developed ELISA was applied to study the structure-activity relationship of ST
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Report
(2 results)
Research Products
(16 results)
