• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of diagnostic methods for acute myocordial infarction using monoclonal antibody

Research Project

Project/Area Number 61870035
Research Category

Grant-in-Aid for Developmental Scientific Research

Allocation TypeSingle-year Grants
Research Field Circulatory organs internal medicine
Research InstitutionUniversity of Tokyo

Principal Investigator

YAZAKI Yoshio  University of Tokyo, 医学部(病), 講師 (20101090)

Co-Investigator(Kenkyū-buntansha) 石毛 雅夫  ヤマサ醤油(株), 研究開発部, 研究員
SUGI Masato  YAMASA Research Institute, 研究開発部, 主任
TSUCHIMOCHI Hidetsugu  University of Tokyo, 医学部(病), 助手 (90197715)
ISOBE Mitsuzaki  University of Tokyo, 医学部(病), 助手 (80176263)
YAMAOKI Kazuhide  University of Tokyo, 医学部(病), 助手 (70182409)
ISHIGE Masao  YAMASA Research Institute
Project Period (FY) 1986 – 1987
Project Status Completed (Fiscal Year 1987)
Budget Amount *help
¥11,800,000 (Direct Cost: ¥11,800,000)
Fiscal Year 1987: ¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 1986: ¥8,300,000 (Direct Cost: ¥8,300,000)
Keywordscardiac myosin / monoclonal antibody / acute myocardial infarction / Imaging of infarction / ミオシン軽鎖測定による心筋梗塞診断 / ラジオイムノメトリックアッセイ / 心筋梗塞生化学的診断法 / ミオシン重鎖 / ミオシン軽鎖
Research Abstract

Estimating the size and location of infarct is important in predicting the prognosis and determining the therapeutic strategy for patients with acute myocardial infarction. Monoclonal antibodies are Known to be highly specific and uniform and can be produced in quantity. Based on these characteristics, we developed a new diagnostic method for myocardial infarction which has excellent specificity and quantitativeness. Noting that cardiac myosin is a major structural portein specific to the myocardium and especially its subunits, light chain and hevay chain, have different properties, we prepared corresponding monoclonal antibodies. Utilizing the fact that these antibodies specific for heavy chain accumulate bound to the myofibril of the infarcted myocardium, we demonstrated through experiments that highly specific and clear image of myocardial infarction can be obtainedby intravenous administration of isotope labelled monoclonal antibodies. We also established a very sensitive microassay using antibodies which specifically bind the light chains liberated from the myocyte into serumfollowin ischemic events, and developed a method for biochemically diagnosing myocaridial infarction. Now that we can obtain accurate information regarding the size of the infarct which would be otherwise impossible by the conventional method, a wide range of clinical application is expected for this method.

Report

(2 results)
  • 1987 Final Research Report Summary
  • 1986 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] Makoto-Kuroo,et al.: J.Clin.Invest.77(2). 340-347 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Issei-Komuro,et al.: J.Biol.Chem.261(10). 4540-4509 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Yosio-Yazaki,et al.: Asian Med.J.29(9). 510-521 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Yosio-Yazaki,et al.: Jpn.Circ.J.51(6). 676-681 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Mitsuaki-Isobe,er al.: Circuration. 76(6). 1251-1261 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Hidetsugu-Tsuchimochi,et al.: J.Clin.Invest.81(1). 110-118 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Yosio-Yazaki,et al.: "Biochemical Imaging" Academic Press Inc., 8 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Yoshio-Yazaki,et al.ed.by.N.S.Dhalla,et al.: "Distribution of cardiac myosin isozymes in human and rat heart in "Heart Function and Metabolism"" Martinus Nijoff Publishing,Boston, 12 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Makoto-Kuroo, et al.: "Distribution of cardiac myosin isozymes in human conduction system; immunohistochemical study using monoclonal antibodyies." J. Clin. Invest.77(2). 340-347 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Issei-Komuro, et al.: "Isolation and characterization of two isozymes of mosion heavy chain from canine atrium." J. Biol. Chem.261(10). 4504-4509 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Yosio-Yazaki, et al.: "Diagnosis of myocardial infraction usiing monoclonal antibodies." Asian Med.J.29(9). 510-521 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Mitsuaki-Isobe, et al.: "Quantitative relationship between left ventricular function and serum cardiac myosin light chain I levels after coronary reperfeesion in patients with acute myocardial infarction." Circulation. 76(6). 1251-1261 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Hdietsugu-Tsuchimoti, et al.: "Heterogeneity of beta-type myosin isozymes in the human and regulational mechainsms in their expression-immunohistochemical study using monoclonal and bodies." J. Clin. Invest.81(1). 110-118 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Yosio-Yazki, et al.: Martinus Nijhoff Publishing Boston. Distribution of cardiac myosin isozymes in human and rat heart in "Heart Function and Metabolism" ed. by. H.S. Dhalla, et al., 294-305 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Hideo-Kosano,et al.: Clinical Chemistry. 32(2). 346-348 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Mitsuaki-Isobe,et al.: Am.J.Cardiol.57(2). 316-321 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Makoto-Kuroo,et al.: J.Clin.Invest.77(2). 340-347 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Issei-Komuro,et al.: J.Biol,Chem.261(10). 4504-4509 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Yoshio-Yazaki,et al.: Asian Med.J.29(9). 510-521 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Takao-Sugiyama,et al.: Biochem.Biophy.Res.Commun.141. 340-345 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Yoshio-Yazaki,et al.: "Biochemical Imaging" Academic Press Inc., 239-247 (1986)

    • Related Report
      1986 Annual Research Report

URL: 

Published: 1987-03-31   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi