| Project/Area Number |
61870040
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| Research Category |
Grant-in-Aid for Developmental Scientific Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Pediatrics
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| Research Institution | The Tokyo Metropolitan Institute of Medical Science (1988)
National Center of Neurology and Psychiatry (1987)
The University of Tokyo (1986) |
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Principal Investigator |
SUZUKI Yoshiyuki The Tokyo Metropolitan Institute of Medical Science, 副所長 (90010389)
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| Co-Investigator(Kenkyū-buntansha) |
OSHIMA Akihiro The Tokyo Metropolitan Institute of Medical Science, 主任研究員 (20203763)
SAKAKIHARA Yoich Faculty of Medicine, The University of Tokyo, 助手 (10143463)
SAKURABA Hitoshi The Tokyo Metropolitan Institute of Medical Science, 室長 (60114493)
床枝 康伸 東京大学, 医学部, 助手 (10172157)
宮下 俊之 東京大学, 医学部, 助手 (60174182)
大村 清 国立精神, 神経センター神経研究所疾病研究第5部, 室長 (70047348)
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| Project Period (FY) |
1986 – 1988
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| Project Status |
Completed (Fiscal Year 1988)
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| Budget Amount *help |
¥17,900,000 (Direct Cost: ¥17,900,000)
Fiscal Year 1988: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1987: ¥4,500,000 (Direct Cost: ¥4,500,000)
Fiscal Year 1986: ¥11,400,000 (Direct Cost: ¥11,400,000)
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| Keywords | two-dimensional electrophoresis / hereditary dominant disease / fibroblast / 軟骨形成不全症 / 高速液体クロマトグラフィー / 二次元電気泳動法 |
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| Research Abstract |
A rapid and reproducible method for screening of normal and mutant human skin fibroblast proteins was developed using high resolutional two-dimensional electrophoresis, silver staining, and digitizer-personal computer analysis. More than 300 spots were visualized on a gel. Iso-electric points were in the range of pH 3.5-7.5, and molecular weights from 10 to 150 kDa.
The 247 protein spots chosen for further statistic analysis in 12 normal control cell strains showed quantitative rariations of various degrees. Among them, 12 spots were found to have positional or remarkably quantitative variations. However, no definite variations among groups with different ages, sexes, or culture conditions. Mycoplasma infections was found to induce six new protein spots in fibroblasts without changes of other spots. No quantitative or quali-tative changes were found in several genetic diseases including von Recklinghausen disease, tuberous sclerosis, achondrogenesis and some lysosomal storage diseases by this method. How-ever, two new spots appeared in three cases of achondroplasia, one of the most common dominant diseases. The protein patterns were normal in the other four cases. Our present study revealed that a genetic heterogeneity may be present in achondroplasia and this screening system is useful for the study of hereditary dominant diseases in clinical laboratories.
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