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Development of new assay systems for examining the relation between osteoblasts and osteoclasts, and identification of new factors controlling bone metabolism

Research Project

Project/Area Number 61870074
Research Category

Grant-in-Aid for Developmental Scientific Research

Allocation TypeSingle-year Grants
Research Field Functional basic dentistry
Research InstitutionShowa University, School of Dentistry

Principal Investigator

SUDA Tatsuo  Showa University, Shool of Dentistry, 歯学部, 教授 (90014034)

Co-Investigator(Kenkyū-buntansha) TANAKA Hirofumi  Showa University, Shool of Dentistry, 歯学部, 助手 (30146899)
MIYAURA Chisato  Showa University, Shool of Dentistry, 歯学部, 助手 (20138382)
TAKAHASHI Naoyuki  Showa University, Shool of Dentistry, 歯学部, 講師 (90119222)
SHINKI Toshimasa  Showa University, Shool of Dentistry, 歯学部, 講師 (90138420)
ABE Etsuko  Showa University, Shool of Dentistry, 歯学部, 助教授 (70119147)
滝戸 次郎  昭和大学, 歯学部, 助手 (00197237)
Project Period (FY) 1986 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥7,100,000 (Direct Cost: ¥7,100,000)
Fiscal Year 1988: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1987: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1986: ¥3,900,000 (Direct Cost: ¥3,900,000)
Keywords1alpha,25-dihydroxyvitamin D_3 [1alpha,25(OH)_2D_3 / differentiation-inducing factor (DIF) / macrophage fusion factor (MFF) / osteoblasts / 破骨細胞 / マクロファージ融合因子(MFF) / 骨髄細胞 / コロニー形成因子(CSF) / 活性型ビタミンD / 副甲状腺ホルモン / カルシトニン
Research Abstract

The aim of the present study was to develop new assay systems for examining the relation between osteoblasts and osteoclasts and to identify new factors controlling bone metabolism. In order to address these problems, we performed following experiments: (1) isolation and characterization of osteotropic factors produced by osteoblasts, (2) development of new assay systems for examining osteoclast formation in vitro, and (3) clarification of the role of osteoblasts in osteoclast formation.
1. We previously reported that osteoblastic MC3T3-E1 cells produced differentiation inducing factor (DIF) which promoted differentiation of mouse myeloid leukemia cells (M1) into macrophage-like cells. The DIF was partially purified from the conditioned meda of MC3T3-E1 cells and its biological activity in bone resorption was examined. The DIF exhibited a marked bone resorbing activity in a Raisz's assay system and it stimulated osteoclast-like cell formation in a mouse marrow culture system.
2. We devel … More oped a mouse bone marrow culture system to examine osteoclast formation in vitro. Bone resorption-stimulating hormones and factors such as 1alpha,25(OH)_2D_3, PTH, PGE_2, IL-1, TNFalpha, and TGF markedly stimulated the formation of osteoclast-like multinucleated cells (MNCs), whereas calcitnin and IFN<@2Y<@D2 strongly inhibited the formation induced by those hormones and factors.
3. In order to examine the role of osteoblasts in osteoclast formation, we developed a co-culture system of mouse spleen cells and osteoblastic cells freshly isolated from fetal mouse calvariae. When mouse spleen cells were co-cultured with osteoblastic cells in the presence of 1alpha, 25(OH)_2D_3, osteoclast-like MNCs were formed within 8 days. Neither the same co-culture without the vitamin nor separate cultures of either spleen cells or osteoblastic cells with the vitamin produced osteoclast-like MNCs. These results indicate that osteoblasts are required for differentiation of osteoclast progenitors into multinucleated osteoclasts. Less

Report

(3 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1986 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] N.Takahashi: Endocrinology. 122. 1373-1382 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] N.Takahashi: Endocrinology. 123. 2600-2602 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] E.Abe: Journal of Bone and Mineral Research. 3. 635-645 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] C.Miyaura: FEBS Letters. 234. 17-21 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] H.Tanaka: Experimental Cell Research. 180. 72-83 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] T.Akatsu: Journal of Bone and Miheral Research. (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 須田立雄: "口腔生化学" 医歯薬出版, 305 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 須田立雄: "骨吸収に関する諸問題-基礎と臨床-" 西村書店, 113 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Naoyuki,Takahashi: "Osteoclast-like cell formation and its regulation by osteotropic hormones in mouse bone marrow cultures." Endocrinology. 122. 1373-1382 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Naoyuki,Takahashi: "Osteoblastic cells are involved in osteoclast formation." Endocrinology. 123. 2600-2602 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Etsuko,Abe: "A differentiation-inducing factor produced by the osteoblastic cell line MC3T3-El stimulates bone resorption by promoting osteoclast formation." J. Bone Mineral Res.3. 635-645 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Chisato,Miyaura: "Recombinant human interleukin 6 (B-cell stimulatory factor 2) is a potent inducer of differentiation of mouse myeloid leukemia cells (M1)." FEBS Lett.234. 17-21 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Hirofumi,Tanaka: "Spermidine-dependent proteins are involved in the fusion of mouse alveolar macrophages induced by lalpha,25-dihydroxyvitamin D_3 and interleukin 4." Exp. Cell Res.180. 72-83 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] N.Takahashi: Enclocrinology. 123. 2600-2602 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] C.Miyaura: FEBS Letters. 234. 17-21 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] E.Abe: Journal of Bone and Mineral Research. 3. 635-645 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] H.Tanaka: Experimental Cell Research. 180. 72-83 (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] T.Akatsu: Journal of Bone and Mineral Research. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] C.Miyaura: Biochemical and Biophysical Research Communications. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Etsuko Abe et al.: Proc.Natl.Acad.Sci.USA. 83. 5958-5962 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Chisato Miyaura et al.: J.Bone Mineral Res.1. 359-367 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Yoshiko Shiina-Ishimi et al.: Biochem.Biophys.Res.Commun.134. 400-406 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Tatsuo Suda et al.: "Bone and Mineral Research vol.4 chapter 1 ppl-46" Elsevier Science Publishers B.V., 419 (1986)

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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