| Project/Area Number |
61870074
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| Research Category |
Grant-in-Aid for Developmental Scientific Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Functional basic dentistry
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| Research Institution | Showa University, School of Dentistry |
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Principal Investigator |
SUDA Tatsuo Showa University, Shool of Dentistry, 歯学部, 教授 (90014034)
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| Co-Investigator(Kenkyū-buntansha) |
TANAKA Hirofumi Showa University, Shool of Dentistry, 歯学部, 助手 (30146899)
MIYAURA Chisato Showa University, Shool of Dentistry, 歯学部, 助手 (20138382)
TAKAHASHI Naoyuki Showa University, Shool of Dentistry, 歯学部, 講師 (90119222)
SHINKI Toshimasa Showa University, Shool of Dentistry, 歯学部, 講師 (90138420)
ABE Etsuko Showa University, Shool of Dentistry, 歯学部, 助教授 (70119147)
滝戸 次郎 昭和大学, 歯学部, 助手 (00197237)
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| Project Period (FY) |
1986 – 1988
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| Project Status |
Completed (Fiscal Year 1988)
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| Budget Amount *help |
¥7,100,000 (Direct Cost: ¥7,100,000)
Fiscal Year 1988: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1987: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1986: ¥3,900,000 (Direct Cost: ¥3,900,000)
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| Keywords | 1alpha,25-dihydroxyvitamin D_3 [1alpha,25(OH)_2D_3 / differentiation-inducing factor (DIF) / macrophage fusion factor (MFF) / osteoblasts / 破骨細胞 / マクロファージ融合因子(MFF) / 骨髄細胞 / コロニー形成因子(CSF) / 活性型ビタミンD / 副甲状腺ホルモン / カルシトニン |
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| Research Abstract |
The aim of the present study was to develop new assay systems for examining the relation between osteoblasts and osteoclasts and to identify new factors controlling bone metabolism. In order to address these problems, we performed following experiments: (1) isolation and characterization of osteotropic factors produced by osteoblasts, (2) development of new assay systems for examining osteoclast formation in vitro, and (3) clarification of the role of osteoblasts in osteoclast formation.
1. We previously reported that osteoblastic MC3T3-E1 cells produced differentiation inducing factor (DIF) which promoted differentiation of mouse myeloid leukemia cells (M1) into macrophage-like cells. The DIF was partially purified from the conditioned meda of MC3T3-E1 cells and its biological activity in bone resorption was examined. The DIF exhibited a marked bone resorbing activity in a Raisz's assay system and it stimulated osteoclast-like cell formation in a mouse marrow culture system.
2. We devel
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oped a mouse bone marrow culture system to examine osteoclast formation in vitro. Bone resorption-stimulating hormones and factors such as 1alpha,25(OH)_2D_3, PTH, PGE_2, IL-1, TNFalpha, and TGF markedly stimulated the formation of osteoclast-like multinucleated cells (MNCs), whereas calcitnin and IFN<@2Y<@D2 strongly inhibited the formation induced by those hormones and factors.
3. In order to examine the role of osteoblasts in osteoclast formation, we developed a co-culture system of mouse spleen cells and osteoblastic cells freshly isolated from fetal mouse calvariae. When mouse spleen cells were co-cultured with osteoblastic cells in the presence of 1alpha, 25(OH)_2D_3, osteoclast-like MNCs were formed within 8 days. Neither the same co-culture without the vitamin nor separate cultures of either spleen cells or osteoblastic cells with the vitamin produced osteoclast-like MNCs. These results indicate that osteoblasts are required for differentiation of osteoclast progenitors into multinucleated osteoclasts. Less
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