| Project/Area Number |
61870102
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| Research Category |
Grant-in-Aid for Developmental Scientific Research
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| Allocation Type | Single-year Grants |
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| Research Field |
医学一般
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| Research Institution | Kyushu University |
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Principal Investigator |
SHIMIZU Nobuaki (1988) Faculty of Medicine,Kyushu University. Lecturer, 医学部, 講師 (50019634)
大村 裕 (1986-1987) 九州大学, 医学部, 教授 (30019517)
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| Co-Investigator(Kenkyū-buntansha) |
前田 満雄 三菱電機, 中央研究所, 主幹
MATSUO Masayuki Tohoku University. Emeritus Professor, 名誉教授 (50005170)
YAMAZOE Noboru Interdisciplinary Graduate School of Ecgineering Sciences, Kyushu University. Pr, 総合理工学研究科, 教授 (40037817)
AKAIKE Norio Faculty of Medicine, Kyushu University. Associate Professor, 医学部, 助教授 (30040182)
OOMURA Yutaka Kyushu University. Emeritus Professor, 名誉教授 (30019517)
MAEDA Mitsuo Central Research Labratory, Mitsubishi Electric Corporation. Group Manager
片渕 俊彦 九州大学, 医学部, 助手 (80177401)
清水 宣明 九州大学, 医学部, 講師 (50019634)
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| Project Period (FY) |
1986 – 1988
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| Project Status |
Completed (Fiscal Year 1988)
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| Budget Amount *help |
¥27,500,000 (Direct Cost: ¥27,500,000)
Fiscal Year 1988: ¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 1987: ¥12,500,000 (Direct Cost: ¥12,500,000)
Fiscal Year 1986: ¥11,000,000 (Direct Cost: ¥11,000,000)
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| Keywords | Biosensor / voltammetry / carbon fiber electrode / DOPAC / 5-HIAA / lateral hypothalamic area / 神経伝達物質 / ボルタメトリー法 / 視床下部外側野 / 摂食行動 / グルコセンサー / 血糖値 / 5-ハイドロキシトリプトファン / 視床下部 |
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| Research Abstract |
The purpose of this project was to develop multifunctional biosensors which can detect local kinetics of endogenous substances influencing neuronal activity in the central nervous system. New type of biogenic amine sensors using voltammetry system was developed. We examined characteristics of the sensors in vitro and tried to apply in vivo conditions.
As a working electrode of in vivo voltammetry, carbon fiber electrodes were used. These electrodes were electrochemically activated beforehand by applying a trinagular current pulses (0-2V, 70 Hz, 60 sec)in 0.1 M H SO sulution. Calibration curves (oxidation current vs. concentration) of DOPAC (metabolite of dopamine) and 5-HIAA (metabolite of serotonin) using these electodes were confirmed to be linear between 1muM and 10muM concentration.
To evaluate the function of probe, serotonin release in the lateral hypothalamic area (LHA; feeding center) was measured continuously in freely moving rats. The carbon fiber electrode was combined with teflon-coated platinum-iridium wires which enabled to record the neuronal activity for long period. The results indicated that the serotonin release in the LHA began to increase at around 1800 hr and reached to the maximum level at around 2100 hr, then gradually decreased and returned to the level at around 1800 hr within 3 or 4 hours. Thirty neurons recorded simultaneously with serotonin release, 12 neurons (40%) increased their activity, 7 (23%) decreased and 11 (37%) showed no change. The results indicate that this kind of probe is useful for analysis of the brain functions with measurements of bioactive substances.
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