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Electrochemical Micro-immunosensors Using Monoclonal Antibodies

Research Project

Project/Area Number 61870104
Research Category

Grant-in-Aid for Developmental Scientific Research

Allocation TypeSingle-year Grants
Research Field 医学一般
Research InstitutionKumamoto University

Principal Investigator

TANIGUCHI Isao  Kumamoto University, 工学部, 助教授 (90112391)

Co-Investigator(Kenkyū-buntansha) 宇野木 正憲  化学及び血清療法研究所, 菊池研究所, 化学研究室室長
TSUJI Ichiro  The Chemo-Sero-Therapeutic Research Institute, 菊池研究所, 研究員
YAMAKAWA Takeshi  Kumamoto University, 工学部, 助教授 (00005547)
YASUKOUCHI Kazuo  Kumamoto University, 工学部, 教授 (60040359)
UNOKI Masanori  The Chemo-Sero-Therapeutic Research Institute
Project Period (FY) 1986 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥5,900,000 (Direct Cost: ¥5,900,000)
Fiscal Year 1988: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1987: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1986: ¥2,300,000 (Direct Cost: ¥2,300,000)
KeywordsElectrochemical Immunosensor / Monoclonal Antibody / Immnoglobulin G / Human Chorionic Gonadotropin (hCG) / Electropolymerization / Polytyramine Thin Film / Modified Electrode / 酵素免疫測定 / 電解電合 / 差動出力 / 微小電極 / 免疫センサ / 電位応答 / イムノグロブリンG / 性腺刺激ホルモン / 電導性薄膜
Research Abstract

The objectives of the present research project have been to develop highly functional and micro immunosensors, and also to study fundamental aspects on such sensors for practical use. In the present study, electropolymerized films have,at the first time, been applied successfully as functional materials to prepare electrochemical immunosensors as described below.
Potentiometric human chorionic gonadotropin (hCG) sensor: when tyramine (=p-(2-aminoethy1)-phenol) is polymerized from an alkline alcohol solution at a low current density (e.g., 60muA/cm^2), a pore-free and fine-textured film with low conductivity was obtained. Using this polytyramine modified electrode ( =0.5, =1-5 mm), on which monoclonal anti-hCG (against hCG- -subunit from mouse ascites fluid) was immobilized, a potentiometric hCG hormone sensor was prepared, which responded selctively, rapidly (< 5 min. for 90% response) and sensitively (detection limit of 1 ng/ml). also, using an another monoclonal antibody immobilized e … More lectrode as a reference one, non-immunochemical response was canceled and down to 1 ng/ml of hCG in a human serum was again determined.
Highly sensitive enzyme immunosensor for human immunogloblin G (IgG): A porous polytyramine film was formed on a Pt electrode at first from an acid solution and the surface was further covered with a polytyramine film from alkaline alcohol solution to give a polytyramine doubly coated electrode, on which anti-IgG was then immobilizes through the peptide linkage. By means of the competitive enzyme immunoassay technique, IgG was determined in the concentration range of ca. 10 pg/ml to 1 mg/ml. For the present electrode, a mixture of 0.5%Tween-20, a biological detergent, and 1% bovine serum albumin (BSA) was effective to diminish nonspecific responses.
New diode-type biosensors: Polypyrrole or polyaniline thin film was covered on gold film electrodes with a small gap; this device behaved as a diode becarse the conductivity of the polymer film changed with the oxidation state of the film. This device was applied to develop new biosensors for a flow injection system. Less

Report

(3 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1986 Annual Research Report
  • Research Products

    (31 results)

All Other

All Publications (31 results)

  • [Publications] Isao Taniguchi: Analytical Sciences. 2. 587-588 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Isao Taniguchi: Review of Polarography. 32. 83 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Isao Taniguchi: Extended Abstracts of the 172nd Meeting of the Electrochemical Society,held at Honolulu. 87-2. 2213 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Isao Taniguchi: Denki Kagaku. 56. 785-786 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Isao Taniguchi: Denki Kagaku. 56. 1104-1105 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 谷口功: 学術月報. 42. 64 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 谷口功: 病態生理. 8. 243-251 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 辻一郎: 化学センサ研究会論文集. 8. (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ichiro Tsuji: Biosensors,.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Isao Taniguchi: Chem.Lett.,.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Isao Taniguchi: Denki Kagaku,.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Isao Taniguchi: Japanese Sensor Newsletter(Published by Edison Sensor Technology Center,Case Western Reserve University),.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Isao,Taniguchi: "A Potentiometric Immunogloblin G Sensor Based on a Polypyrrole Modified Platinum Electrode" Analytical Sciences. 2. 587-588 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Isao,Taniguchi: "The Potentiometric Immunosensor Based on a Phlytyramine Modefied Electrode" Review of Polarography. 32. 83 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Isao,Taniguchi: "A Ferrocene-Modified Carbon Fiber Microelectrode as a Potentiometric Sensor for Hydrogen Peroxide" Extended Abstract of the 172nd Meeting of the Electrochemical Society, held at Honolulu. 87-2. 2213 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Isao,Taniguchi: "Potentiometric Human Chrionic Gonadotropin (hCG) Sensor Based on Monoclonal Antibody Immobilized Polytyramine Modified Electrode" Denki Kagaku. 56. 785-786 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Isao,Taniguchi: "Application of Polytyramine Modified Electrodes to Amperometric Enzyme Immunosensor for Human Immunogloblin G" Denki Kagaku. 56. 1104-1105 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Isao,Taniguchi: "Recent Developments in Electrochemical Immunosensor" Byotai seiri (Medicina Philosophica). 8. 243-251 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ichiro,Tsuji: "Electrochemical Immunosensors Based on Electrogenerated Polymer Modefied Electrodes" Proc. of The 8th Chemical Sensor Meeting. 8. (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ichiro,Tsuji: "Enzyme Immunosensors for Human Immungloblin G Based on Electropolymerized Polytyramine Modefied Electrodes" Biosensors,.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Isao,Taniguchi: "New Diode-Type Biosensors Using Electropolymerized Thin Films" Denki Kagaku,.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Isao,Taniguchi: "Use of Monoclonal Antibody for Highly Sensitive Immunosensor: A Potentiometric Human Choriomic Gomadotropin (hCG) Sensor" Japanese Sensor Newsletter.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Isao Taniguchi: Denki Kagaku. 56. 785-786 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Isao Taniguchi: Denki Kagaku. 56. 1104-1105 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 谷口功: 学術月報. 42. 64 (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] 谷口功: 病態生理. 8. 243-251 (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Ichiro Tsuji: Biosensors,.

    • Related Report
      1988 Annual Research Report
  • [Publications] Isao Taniguchi: Denki Kagaku,.

    • Related Report
      1988 Annual Research Report
  • [Publications] Isao Taniguchi: Analytical Sciencies. 2. 587-588 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Isao Taniguchi: Rev.of Polarography(Kyoto). 32. 83 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Isao Taniguchi: J.Electroanal.Chem.,.

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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