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DEVELOPMENT OF SPECIFIC ASSAY FOR MEASURING ACTIVE FREE FORM OF thyroid hormones for monitering thyroid function

Research Project

Project/Area Number 61870108
Research Category

Grant-in-Aid for Developmental Scientific Research

Allocation TypeSingle-year Grants
Research Field Laboratory medicine
Research InstitutionOsaka University Medical School

Principal Investigator

MIYAI Ikiyoshi  Department of Laboratory Meficine Oasaka University Medical school, 医学部, 教授 (30028394)

Project Period (FY) 1986 – 1987
Project Status Completed (Fiscal Year 1987)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 1987: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1986: ¥2,400,000 (Direct Cost: ¥2,400,000)
Keywordsenzyme immunoassay / radioimmunoassy / free thyroxine / 遊離型トリヨードサイロニン / 酵素免疫測定法 / 新生児
Research Abstract

New enzyme immunoassay (EIA) and radfioimmunoassay (RIA) for measuring free thyroxind (FI_4) and free triiodothyronine (FT_3) unifluenced by albumin and T_4 binding blobulin (TBG) were developed.
(1) EIA of FT_4 A double-antibody EIA for determination of FT_4 in serum and dried blood samples on filter paper was developed with use of a T_4-<beta>-D-galactosidase complex. The measurable range of FT_4 concentration in two 3-mm blood discs, was 1.9 to 93 ng/L. The mean coefficients of variation (CV) were 7.6%(within assay) and 6.4%(berween assays.) Results for FT_4 by this method correlated well with those for serum determined by RIA. (2) RIA of FT_4 A double-antibody solid phase RIA for FT_4 in serum with use of^<125> I-labelled T_4-hcg conjugate was developed. Since the labelled conjutage does not bind to TBG and albumin, the method is uninfuenced by TBG or albumin. The measurable of FT_4 serum was 2.0 to 128 ng/l. The mean CV within and between assays were 4.6-8.6% and 6.3-11.6%, respect … More ively. The FT_4 concentrations is serum as determined by this method were 9 to 17 ng/l for healthy adult subjects; high for pationts with hyperthyroidism; low for patients with hypothroidism; and within normal limits for pregnant women, and parients tiwh high or low concentrations of TBG. (3) EIA of FT_3 In the new solid-phase antibody EIA for FT_3 in serum, <beta>-D-galactosidase conjugated to T_3 is use. Results are uninfulenced by physiological concentrations og TBG or albumin, Results correlate well with those determined by equilibrum dialysis. The mean CVs within and between assays were 6.1 and 9.5%, respectively. The measurable range of FT_3 in serum is 0.7 to 26 ng/L; the normal refernce interval is 1.9 to 8.9 nb/L. Concentrations of FT_3 in serum of patients with hyperthyoidism were high; those of patients with hypothyroidism were within normal limits or low, and those of patients with congenitally decteased ot increased TBG were within the normal range. These methods are useful for monitoring thyroid function. Less

Report

(2 results)
  • 1987 Final Research Report Summary
  • 1986 Annual Research Report
  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] N.Hata et al.: Clin.Chem.33. 172-176 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] S.Tsutsumiet al.: Clin. Chim. Acta. 170. 315-322 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] K.Miyai et al.Ed G.Mederios Nelo,E Gaitan: "Measurement of "free thyroxine" in dried blood samples onfilter paper by radio-and enzyme-immunoassay for monitering thyroid function: In Frontiers in Thyroidology" Plenum Medical Book Company, New York, 4 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] K.Miyai et al.: "Free thyroxine. In Methods of Enzymatic Analysis." VCH Verlagsgesellschaft., 13 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] N. HATA, et al.: "Enzyme immunouassay of free triiodothronine in serum." Clin. Chem.33. 172-176 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] S. Tsutsumi et al.: "A new radioimmunoassay of free thryoxine using^<125>l-labbelledtheoxine-protein complex uninfulenced by albumin and thyroxine binding globulin." Clin. Chim. Acta. 170. 315-322 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] K. Miyai er al.: Plenum Medical Book Companr, New York. Measurement of "free thyroxine" in dried blood samples on filter paper by radio-and enzyme-immunoassay for monitering thyroid function: In Frontiers in Thyroidology., 4 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] K. Miyai er al.: VCH Verlagsgesellschaft.thyroxine. In methods of Enzymatic Analysis., 13 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] K.Miyai;M.Ito;N.Hata;Y.Endo;eds,H.V.Bergmeyer;J.Bergmeyer;M.Grassl: "Methods of Enzymatic Analysis Third Edition Volume IX Proteins ans Peptides" VCH.Verlagsgesellshaft mbH, 13 (1986)

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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