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A developmental model of the computer-assisted HPLC multiwavelength UV detection system for simultaneous toxicological drug analysis

Research Project

Project/Area Number 61870114
Research Category

Grant-in-Aid for Developmental Scientific Research

Allocation TypeSingle-year Grants
Research Field 医学一般
Research InstitutionNippon Medical School

Principal Investigator

WATANABE Tokinori  Nippon Medical School, Dept./f Legal Medicine professor, 医学部(法医学), 教授 (80060333)

Co-Investigator(Kenkyū-buntansha) HONDO Toshinobu  Japan Spectrscopic Co.Ltd technical engineer, 生化学応用技術課, 技術員
遠藤 俊吉  日本医科大学, 医学部(精神医学), 教授 (80147743)
YAMAMOTO Yasuhiro  Nippon Medical School, Dept.of Critical Care Medicine assistant professor, 医学部(救急医学), 助教授 (70125079)
HAYASHIDA Makiko  Nippon Medical School, Dept.of Legal Medicine assistant, 医学部(法医学), 助手 (60164977)
NIHIRA Makoto  Nippon Mediacl school, Dept.of lelgal Medicine lecturer, 医学部(法医学), 講師 (40089636)
ENDO Shunkichi  Nippon Medical School, Dept.of Neuropsychiatry professor
Project Period (FY) 1986 – 1987
Project Status Completed (Fiscal Year 1987)
Budget Amount *help
¥5,000,000 (Direct Cost: ¥5,000,000)
Fiscal Year 1987: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1986: ¥4,000,000 (Direct Cost: ¥4,000,000)
KeywordsEmergency toxicology / Simultaneous drug screening / High pressure liquid chromatography(HPLC) / Identification / Computer-assisted / Multiwaveloength UV detector / Retention prediction / コンピュータ / 高速液体クロマトグラフィ / 向精神薬 / 多剤併用 / スペクトラルライブラリー
Research Abstract

A new emergency drug screening system for separation and identification of the toxic drugs, "MULTI-HPLC" is presented. The HPLC system used comprised a Model 880-PU pump and Multi-320 multichannel UV detector (Jasco). The column was a Jasco FineSil C18S(250mmx4.6mmI.D.) set at 50゜C. The mobile phases were mixtures of 10mM perchloric acid, 10mM sodium perchlorate and acetonitrile (CH_3 CN) and flow-rate was 1ml/min.
Sixty-five toxic drugs, frequently seen in drug poisoning cased in Japan, were selected as the reference drugs. According to the pharmacological effect, they were classified as antipsychoactive, antianxietic, anticonvulsant and antidepressant drugs, while classified as barbiturates, benzadiazaepines, butyrophenones and phenothiazines according to the structure. The data handling was performed by a NEC9801Vm2 personal computer and data processor(DP-L320,Jasco) the data processing functions are three-dimensional chromatogram, contour chromatogram, peak deconvolution and so on. … More UV spectra of the drugs at the apex of the chromatographic peaks were stored in a spectrum library. Also in MULTI-HPLC system could be used an automated identification system based on the retention prediction concept, proposed by jinno(Toyohashi Univ.of Technology, School of Materials Science). The log Pe data file on sixty-five drugs were prepared. Rerention(T_r ), UV spectral, optimum wavelength and detection limit data of each drug were listed. The relation between sixty-five standard drug concentrations and peak area ratio was linear from 1 to 1000ng/ml. Peak deconvolution technique was applied to unresolved multi component peak analysis. Recoveries of sixty-five drugs from serum or urine were examine to compare following four exstraction procedures ; CH_3 CN deproteinization, ULTRA-FREE^Rfiltration, TOXI-TUBE^R extraction, Bond-ELUT^r elution method.
possible application of the system were demonstrated using gastric contents, sera and urines in drug poisoning cases. In most cases at least more than six drugs were identified. Because the poisoned patients were often in a state of deep coma on arrival at Critical Care Medical Center, Nippon Medical School, no information of the toxic substances was obtained at the onset of the analysis. MULTI-HPLC system is considered useful for toxicological drug screening in the cases of "polydrug overdose" or "mulltiple drug ingestion" in which caseshave been unable to bve analyzed by the convetional HPLC analysis. Although the system in its present form will leave many problems to be solve in practice, the concept opens a new dimension of emergency toxicology. Less

Report

(2 results)
  • 1987 Final Research Report Summary
  • 1986 Annual Research Report

Research Products

(40 results)

All Other

All Publications (40 results)

  • [Publications] T.HOSHINO; T.HONDO; M.SEMDA; M.SAITO and S.TOHEI: Journal of Chromatography. 332. 139-146 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] T.HOSHINO; M.SENDA; T.HONDA; M.SAITO and S.TOHEI: Journal of Chromatography. 316. 473-486 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 林田眞喜子,仁平信,平川慶子,渡辺日章 他: 日本法医学雑誌. 39(6). 539 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 林田眞喜子,仁平信,平川慶子,渡辺日章 他: Proceedings of the Japanese Society for Medical Mass Spectrometry. 10. 95-98 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 林田眞喜子,仁平信,平川慶子,渡辺日章: 日本法医学雑誌. 41(6). 669 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 須崎紳一郎,山本保博,林田眞喜子,仁平信,渡辺日章: 第9回中毒研究会抄録集. 9. 32 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 須崎紳一郎,林田眞喜子: 医学のあゆみ. 143(1). 5-9 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 神野清勝,桑島幹,林田眞喜子,渡辺日章,本堂敏信: CHROMATOGRAPHY. 8. 65-66 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] K.JINNO,M.KUWAJIMA,M.HAYASHIDA,T.WATANABE and T.HONDO: Journal of Chromatography. 436. 11-21 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 高木宏昌,倉岡幸令,遠藤俊吉,林田眞喜子,仁平信,渡辺日章: Jpn.Psycgopharmacol.7(1). IN PRESS (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 須崎紳一郎,山本保博,大塚敏文,林田眞喜子,仁平信,渡辺日章: 第15回日本救急医学会抄録集. 106 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 仁平信,林田眞喜子,渡辺日章: 日本法医学雑誌. 42(1). (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 高木宏昌,林田眞喜子: 医学のあゆみ. (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 林田眞喜子,仁平信,渡辺日章,須崎紳一郎,山本保博: 中毒研究. 2. IN PRESS (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 林田眞喜子,仁平信,渡辺日章,神野清勝: 第72次日本法医学会.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 林田眞喜子,仁平信,渡辺日章,須崎紳一郎,山本保博: 第10回急性中毒研究会.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 林田眞喜子,仁平信,渡辺日章,須崎紳一郎,山本保博: 第10回急性中毒研究会.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 林田眞喜子,仁平信,渡辺日章,高木宏昌,倉岡幸令,遠藤俊吉: 第9回日本精神神経薬理学会.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] T.HOSHINO, T.HONDO, M.SENDA, M.SAITO and S.TOHEI: "Quantitative deconvolution of heavily fused cheromatographic peaks of biological component using a multiwavelength UV detector" Journal of Chromatography. 332. 139-146 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] T.HOSHINO, M.SENDA, T.HONDO, M.SAITO and S.TOHEI: "Applications of photodiode array ultraviolet detector to unresolved peak analyusis" Journal of Chromatography. 316. 473-486 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] M.HAYASHIDA, M.NIHIRA, K.HIRAKAWA and T.WATANABE: "Investigation of acute poisoning substances and its medico-legal practice of emergency drug analysis" The Japanese Journal of Legal Medicine. 39(6). 539- (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] M.HAYASHIDA, M.NIHIRA, K.HIRAKAWA and T.WATANABE: "Emergency drug analysis by mass spectrometry(1) Measurement of SIMS/MI spectra of bromureids by linked scan technique" Proceedings of the Japanese Society for Medical Mass Spectrometry. 10. 95-98 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] M.HAYASHIDA, M.NIHIRA, K.HIRAKAWA and T.WATANABE: "Isolation of forty-eight drugs by MULTI-HPLC system" The Japanese Journal of Legal Medicine. 41(6). 669- (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] S.Suzaki and M.HAYASHIDA: "Rapid drug screening for emergency toxicology and its therapeutic spplication" Jornal of Clinical Experimental Medicine. 143(1). 5-9 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] K.JINNO, M.KUWAJIMA, M.HAYASHIDA, T.WATANABE and T.HONDO: "Automated identification of toxic substances in poisoned human fluids by a retention prediction system in reversed-phase liquid chromatography" Journal of Chromatography. 436. 11-21 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] K.JINO, M.KUWAJIMA, M.HAYASHIDA, T.WATANABE and T.HONDO: "Autcmated identification of toxic substances in human fluids by a retention prediction system in reversed-phase liquid chromatography(2nd.report)" CHROMATOGRAPHY. 8. 65-66 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] H.TAKAGI, Y.KURAOKA, S.ENDO, M.HAYASHIDA, M.NIHIRA and T.WATANABE: "Simultanceous HPLC analysis of psychotropic drugs using multiwavelength UV detector" The japanese Journal of Psychopharmacology. 7(1). (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] M.NIHIRA, M.HAYSHIDA and T.WATANABE: "A drug abuse information system in the United States of America" The Japanese Journal of Legal Medicine. 42(1). (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] H.TAKAGI and M.HAYASHIDA: "Some analytical probolems for the drug level monitoring in pollypharmaceutic use" Journal of Clinical Experimental Medicine. (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] M.HAYASHIDA, M.NIHIRA, M.WATANABER, S.SUZAKI and Y.YAMAMOTO: "Simultaneous HPLC analysis of toxicological drugs using multiwavelength UV detector" The Japanese Journal of Toxicology. 2. (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] M.HAYASHIDA, M.NIHIRA, T.WATANABE and K.JINNO: "Simultaneous HPLC analysis of toxicological drugs using multiwavelength UV detector(2nd.report)"

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] M.HAYASHIDA, M.NIHIRA, T.WATANABE, S.SUZAKI and Y.YAMAMOTO: "Changes of the serum drug concentrations in the polydrug overdose cases"

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] M.HAYASHIDA, M.NIHIRA, T.WATANABE, S.SUZAKI and Y.YAMAMOTO: "Rapid drug screening combined TOXI-LAB method and MULTI-HPLC system"

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] M.HAYASHIDA, M.NIHIRA, T.WATANABE, H.TAKAGI, Y.KURAOKA and S.ENDO: "Preparations for psychotropic drug level monitoring using MULTI-HPLC system"

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] T.HOSHINO;T.HONDO;M.SENDA;M.SAITO;S.TOHEI: Journal of Chromatography. 332. 139-146 (1985)

    • Related Report
      1986 Annual Research Report
  • [Publications] T.HOSHINO:M.SENDA;T.HONDO;M.SAITO;S.TOHEI: Journal of Chromatography. 316. 473-486 (1985)

    • Related Report
      1986 Annual Research Report
  • [Publications] 林田眞喜子,仁平信,平川慶子,渡辺日章 他: 日本法医学雑誌. 39(5). 539 (1985)

    • Related Report
      1986 Annual Research Report
  • [Publications] 林田眞喜子,仁平信,平川慶子,渡辺日章 他: Proceedings of the Japanese Society for Medical Mass Spectrometry. 10. 95-98 (1985)

    • Related Report
      1986 Annual Research Report
  • [Publications] 林田眞喜子,仁平信,平川慶子,渡辺日章: 第71次日本法医学会総会.

    • Related Report
      1986 Annual Research Report
  • [Publications] 須崎紳一郎,山本保博,林田眞喜子,渡辺日章: 第9回急性中毒研究会総会.

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-30   Modified: 2016-04-21  

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