| Project/Area Number |
61880023
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| Research Category |
Grant-in-Aid for Developmental Scientific Research
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| Allocation Type | Single-year Grants |
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| Research Field |
分子遺伝学・分子生理学
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| Research Institution | Osaka University |
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Principal Investigator |
MATSUBARA Kenichi (1988) Osaka University,Professor, 細胞工学センター, 教授 (20037394)
室津 知明 (1986-1987) 大阪大学, 助教授 (00110792)
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| Co-Investigator(Kenkyū-buntansha) |
OCHIYA Takahiro Osaka University,Research Associate, 細胞工学センター, 助手 (60192530)
FUKUSHIGE Shinichi Osaka University,Research Associate, 細胞工学センター, 助手 (90192723)
釣本 敏樹 大阪大学, 細胞工学センター, 助手 (30163885)
松原 謙一 大阪大学, 細胞工学センター, 教授 (20037394)
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| Project Period (FY) |
1986 – 1988
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| Project Status |
Completed (Fiscal Year 1988)
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| Budget Amount *help |
¥36,700,000 (Direct Cost: ¥36,700,000)
Fiscal Year 1988: ¥8,200,000 (Direct Cost: ¥8,200,000)
Fiscal Year 1987: ¥8,500,000 (Direct Cost: ¥8,500,000)
Fiscal Year 1986: ¥20,000,000 (Direct Cost: ¥20,000,000)
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| Keywords | Cell Sorter / Dual Laser / 染色体 |
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| Research Abstract |
We have developed chromosome sorting techniques using a dual laser chromosome sorter to analyze the structure of human chromosomes.
Human chromosomes stained with Hoechst 33258 (Ho) and chromomycin A3 (CA3) flowed sequentially through two laser beams, one adjusted to 457.9nm to excite CA3 and the other adjusted to 351.1-363.8nm to excite Ho. The Ho and CA3 contents of individual chromosomes were determined by measuring the intensities of fluorescence that were emitted as they passed two laser beams. These measurements were accumulated to form fluorescence intensity distributions.
Sorting techniques have evolved from the ability to sort 10-20 human chromosome fractions with a single laser system to sorting 21 fractions including 15 unique types with a dual laser system.
dFurthermore, chromosome types that cannot be sorted at high purity from chromosomes isolated from human cells can be sorted from human-hamster hybrid cell lines containing one or a few human chromosomes.
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