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Development of chromosome sorting techniques using a dual laser chromosome sorter

Research Project

Project/Area Number 61880023
Research Category

Grant-in-Aid for Developmental Scientific Research

Allocation TypeSingle-year Grants
Research Field 分子遺伝学・分子生理学
Research InstitutionOsaka University

Principal Investigator

MATSUBARA Kenichi (1988)  Osaka University,Professor, 細胞工学センター, 教授 (20037394)

室津 知明 (1986-1987)  大阪大学, 助教授 (00110792)

Co-Investigator(Kenkyū-buntansha) OCHIYA Takahiro  Osaka University,Research Associate, 細胞工学センター, 助手 (60192530)
FUKUSHIGE Shinichi  Osaka University,Research Associate, 細胞工学センター, 助手 (90192723)
釣本 敏樹  大阪大学, 細胞工学センター, 助手 (30163885)
松原 謙一  大阪大学, 細胞工学センター, 教授 (20037394)
Project Period (FY) 1986 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥36,700,000 (Direct Cost: ¥36,700,000)
Fiscal Year 1988: ¥8,200,000 (Direct Cost: ¥8,200,000)
Fiscal Year 1987: ¥8,500,000 (Direct Cost: ¥8,500,000)
Fiscal Year 1986: ¥20,000,000 (Direct Cost: ¥20,000,000)
KeywordsCell Sorter / Dual Laser / 染色体
Research Abstract

We have developed chromosome sorting techniques using a dual laser chromosome sorter to analyze the structure of human chromosomes.
Human chromosomes stained with Hoechst 33258 (Ho) and chromomycin A3 (CA3) flowed sequentially through two laser beams, one adjusted to 457.9nm to excite CA3 and the other adjusted to 351.1-363.8nm to excite Ho. The Ho and CA3 contents of individual chromosomes were determined by measuring the intensities of fluorescence that were emitted as they passed two laser beams. These measurements were accumulated to form fluorescence intensity distributions.
Sorting techniques have evolved from the ability to sort 10-20 human chromosome fractions with a single laser system to sorting 21 fractions including 15 unique types with a dual laser system.
dFurthermore, chromosome types that cannot be sorted at high purity from chromosomes isolated from human cells can be sorted from human-hamster hybrid cell lines containing one or a few human chromosomes.

Report

(3 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1986 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] Fukushige,Shinichi: Biochem.Biophys.Res.Commun.134. 477-483 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Fukushige,Shinichi: Mol. Cell.Biol.6. 955-958 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tokino,Takashi: J.Virol.61. 3848-3854 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Horii,Akira: Biochem.Biophys.Res.Commun.149. 635-641 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 福重真一: 細胞工学. 7. 896-903 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 中村紀彦: 大阪大学医学雑誌.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Fukushige,Shinichi: "Chromosomal assignment of human genes for gastrin, thyrotropin (TSH)- subunit and c-erbB-2 by chromosome sorting combined with velocity sedimentation and Southern hybridization" Biochem.Biophys.Res.Commun.134. 477-483 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Fukushige,Shinichi: "Localization of a novel v-erbB-related gene, c-erbB-2, on human chromosome 17 and its amplification in a gastric cancer cell line" Mol.Cell.Biol.6. 955-958 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tokino,Takashi: "Chromosomal translocation and inverted duplication associated with integrated hepatitis B virus in hepatocellular carcinomas" J.Virol.61. 3848-3854 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Horii,Akira: "Primary structure of human pancreatic secretory trypsin inhibitor (PSTI) gene" Biochem.Biophys.Res.Commun.149. 635-641 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Nakamura,Norihiko: "Identification of human chromosomes using human endogenous retroviral LTRs as marker probes" Medical Journal of Osaka University Japanese Edition,.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Semba,Kentaro: Jpn.J.Cancer Res.(Gann). 79. 710-717 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Miyajima,Nobuyuki: Nucleic Acids Research. 16. 11057-11074 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 福重真一: 細胞工学. 7. 896-903 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 中村紀彦: 大阪大学医学雑誌.

    • Related Report
      1988 Annual Research Report
  • [Publications] Fukushige,Shinichi: Biochem.Biophujs.Res.Comm.134. 477-483 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Takahashi,Yooshuke: Gene. (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] 室津知明: 組織培養. 12. 347-351 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Ohtsubo,Motoki: Molec.Cell.Biol.

    • Related Report
      1986 Annual Research Report
  • [Publications] 福重真一: 続生化学実験講座1 遺伝子研究法【III】(日本生化学会編). 301-313 (1987)

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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