• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Study of the planar membrane on the silica compounds and its application

Research Project

Project/Area Number 61880024
Research Category

Grant-in-Aid for Developmental Scientific Research

Allocation TypeSingle-year Grants
Research Field 生物物性学
Research InstitutionUniversity of Tokyo

Principal Investigator

NAKANISHI Mamoru  Faculty of Phamaceutical Sci., University of Tokyo, 薬学部, 助手 (90090472)

Co-Investigator(Kenkyū-buntansha) TAKAHASHI Seizo  Department of Chemistory, Japan Women's University, 家政学部, 助教授 (00011693)
Project Period (FY) 1986 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥4,100,000 (Direct Cost: ¥4,100,000)
Fiscal Year 1988: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1987: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1986: ¥2,500,000 (Direct Cost: ¥2,500,000)
KeywordsLipid hapten / Planar membrane / FET / Antibody / LB膜 / ラングミュアブロジェット法 / ナノ秒蛍光 / スペーサー / ラングミュア・ブロジェット / 免疫センサー / マクロファージ / 蛍光エネルギー移動
Research Abstract

We have studied the antibody-dependent binding of macrophages to supported planar lipid monolayers containing haptented phospholipids. The binding of macrophages to planar DMPC or DPPC monolayers was dependent on IgG subclasses and hapten concentrations. The binding efficiencies were as follows on both 'solid' and 'fluid' lipid monolayer membranes; IgGl IgG2a IgG2b IgG3. Next, we determined the location of haptens (TNP and dansyl) in lipid bilayer membranes by nanosecond fluorometry. It was found that TNP (or dansyl) residues of lipid haptens locate at the similar vertical position on the membrane surfaces even if they have different length spacers. From these kinds of experiments it was explained how the spacer length can modulate the binding affinity of antibody molecules to the lipid hapten in the membrane. Lastly, we have studied a new type of immuno-FET which has lipid membranes on the surface of an ISFET gate (SI_3N_4/SiO_2) by the fusion of single unilamella liposomes containing lipid haptens (TNP-Cap-DPPE). When mouse monoclonal anti-TNP IgGl was added to the solution, a time-dependent potential change (5-10mV) towards negative was odsedved by a pH/pCO_2 monitor. As for a control experiment, non-specific muse IgGl was added to the solution, no potential change was observed.

Report

(3 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1986 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] 中西守: 代謝. 24. 209-215 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kobayashi,Y.;Utsunomiya,N.;Nakanishi,M.;Osawa,T.: Immunology Lett.15. 53-57 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kato,K.;Nakanishi,M.;Arata,Y.;Miyamoto,H.: J.Biochem.102. 1-4 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Mizuguchi,J.;Utsunomiya,N.;Nakanishi,M.;Arata,Y.: J.Immunol.140. 2495-2499 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 中西守: 実験医学. 6. 133-137 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Utsunomiya,N.;Nakanishi,M.;Arata,Y.;Tadakuma,T.: J.Immunol.141. 1471-1475 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kimura,K.; Nakanishi,M.; Ueda,M.; Yasuda, T.: "The Effect of Immunoglobulin Gl Structure on Macrophage Binding to Supported Planar Lipid Monolayers" Immunology. 59. 235-238 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kobayashi, Y.; Utsunomiya, N.; Nakanishi, M.; Osawa, T.: "Early Transmenbrane Events in Tumor Necrosis Factor and Lymphotoxininduced Cytotoxicity" Immunology Lett.15. 53-57 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kato,K.; Nakanishi,M.; Arata,Y.; Miyamoto,H.: "Calcium Influx in a Single Rat Basophilic Leukemia Cell as Revealed with a Digital Imaging Fluorescence Microscope." J. Biochem.102. 1-4 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kuwana,Y.; Nakanishi,M.; Arata,Y.; Kurosawa,Y.: "Expression of Chimeric Receptor Composed of Immunoglobulin-derived V Regions and T-cell Receptor-derived C Regions." Biochem. Biophys. Res. Commun.,. 149. 960-968 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Mizuguchi,J.; Utsunomiya,N.; Nakanishi,M.; Arata,Y.: "Phorbol Myristate Acetate Inhibits Increase in Membrane Fluidity Induced by Anti-IgM in B Cells." J. Immunology. 140. 2495-2499 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Mizuguchi,J.;Utsunomiya,N.;Nakanishi,M.;Arata,Y.: J.Immunol.140. 2495-2499 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Utsunomiya,N.;Nakanishi,M.;Arata,Y.;Tadakuma,T.: J.Immunol.141. 1471-1475 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 中西守: 実験医学. 6. 133-137 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 中西守: 島津科学機器ニュース. 29. 10-14 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 中西守: 化学と薬学の教室(バイオテクノロジー特集). 81-85 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 中西守: 細胞工学. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] 中西守: "生命科学の基礎6.(抗原認識機講)" 学会出版センター, (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] 中西守: 生物物理. 26. 130-137 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] N.Utsunomiya,M.Tsuboi,M.Nakanishi: Proc.Natl.Acad.Sci.USA. 83. 1877-1880 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] N.Utsunomiya,M.Tsuboi,M.Nakanishi: Biochemistry. 25. 2582-2584 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] 中西守: 薬学研究の進歩. 2. 202-213 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] N.Utunomiya,M.Nakanishi: J.Biol.Chem.261. 716514-1651 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] K.Kimura,M.Nakanishi: Immunology. 59. 235-238 (1986)

    • Related Report
      1986 Annual Research Report

URL: 

Published: 1987-03-31   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi