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Development of a method of "Voltage Clamp by light" for the study of visual neurons

Research Project

Project/Area Number 61890013
Research Category

Grant-in-Aid for Developmental Scientific Research

Allocation TypeSingle-year Grants
Research Field 広領域
Research InstitutionKYUSHU INSTITUTE OF TECHNOLOGY (1988)
Okazaki National Research Institutes (1986-1987)

Principal Investigator

YASUI Syozo  Kyushu Institute of Technology, Department of Control Engineering and Science, Professor, 情報工学部, 教授 (50132741)

Co-Investigator(Kenkyū-buntansha) KANEKO Akimichi  National Institute for Physiological Sciences, Professor, 生理学研究所, 教授 (00051491)
Project Period (FY) 1986 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥5,500,000 (Direct Cost: ¥5,500,000)
Fiscal Year 1988: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1987: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1986: ¥3,700,000 (Direct Cost: ¥3,700,000)
Keywordsvision / color vision / retina / photoreceptor / horiqontal cell / synapse / phototransduction / voltage clamp / Na-Kポンア / 膜抵抗 / 膜電位固定 / 色臨 / NaーCa交換 / NaーKポンプ / Na-Kポンプ
Research Abstract

The method of voltage clamp has provided physiology with a basic experimental paradigm for mofe than three decades. It is not, however,feasible in some cases, due to problems arising from the cell morphology (size and shape) , electrical connection with other cells, and etc. This is true with many visual neurons, and often one can learn little about important subjects such as synaptic mechanisms to mediate the photo-response. The purpuse of this research was to get around the problem by developing a new voltage clamp method which makes use of the natural stimulus to the visual system. namely light. We feel that the aim has adequately been attained, as is summarized below.
1. Development of the Method of " Voltage Clamp by Light "
(1.1) Protype System: a servomechanical apparatus was designed and fabrica ted, which automatically controls the light intensity on the basis of membrane voltage error signal.
(1.2) Extended System: the bridge-balance method was incorporeted with the preceding prototype, to allow simultaneous measurement of the membrane resistance under the voltage clamp by light.
2. Application Research i: the eqipment of (1.1) was used to measure DC and AC spectral sensitivities of horizontal cells in the carp retina. A new observation was made from this result, bearing close relevance to the finding of 3.
3. Application Research ii: the system of (1.2) was used to investigate the synaptic mechanisms from cone photoreceptors to horizontal cells. The result necessitates a revision on a popular notion in this field.
4. Application Research iii: the apparatus of (1.2) was applied to find a new phenomenon asscociated with the process of light adaptation in the crayfish photoreceptor cell.

Report

(3 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1986 Annual Research Report
  • Research Products

    (41 results)

All Other

All Publications (41 results)

  • [Publications] 山田雅弘,安井湘三,藤本正昭: 第2回生体・生理工学シンポジウム論文集(計測自動制御学会). BPES. 157-160 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] S.Yasui: Neuroscience Research. Suppl.6. S133-S146 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] M.Yamada & S.Yasui: Journal of Neuroscience Methods. 24. 65-72 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 安井湘三,山田雅弘: 電子情報通信学会技術報告. MBE87-119. 131-134 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] S.Yasui: Biomedical Research. 9(Supp1.2). 119-123 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] S.Yasui & M.Yamada: Experimental Brain Research. 74. 256-262 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] S.Yasui & M.Yamada: Proceeding of the lnternational Union of Physiological Sciences. XV1. 421-421 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] S.Yasui & M.Yamada: Neuroscience Research. Supp1.3. S53-S53 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 山田雅弘,安井湘三: 医用電子と生体工学. 24特別号. 503-503 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] S.Yasui & M.Yamada: Neuroscience Research. Supp1.5. S186-S186 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 山田雅弘,安井湘三,重松征史: 第64回日本生理学会大会予稿集. 320-320 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] M.Yamada,M.Fujimoto & S.Yasui: Journal of the Physiological Society of Japan. 50. 499-499 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 安井湘三,山田雅弘,Djamgoz.M.B.A: 第66回日本生理学会予稿集. 242-242 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] M.Yamada,S.Yasui & M.B.A Djamgoz: Proceedings of the International Congress of Physiological Sciences XXXI. (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] M. Yamada; S. Yasui; M. Fujimoto: "The Membrane Resistance of Crayfish Photoreceptors Increases Slowly During Constant Depolarization Maintained by the Voltage Clamp by Light" The Second Symposium on Biological and Physiological Engineering. BPES. 157-160 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] S. Yasui: "Ca and Na homeostasis in horizontal cells of the cyprinid fish retina : Evidence for Na-Ca exchanger and Na-K pump" Neuroscience Research. Suppl. 6. 133-146 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] M. Yamada; S. Yasui: "Measurement of DC and AC spectral sensitivities of refinal horizontal cells by "voltage clamp by light"" Journal of Neuroscience Methods. 24. 65-72 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] S. Yasui; M. Yamada: "Unusual Synaptic Mechanisms in the Retina" Japan Soc. Electronics & Communication Eng. Technical Report. MBE87-119. 131-134 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] S. Yasui: "Na-Ca exchanger, Na-K ATP ase and calcium stores in retinal horizontal cells" Biomedical Research. 9(Suppl.2). 119-123 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] S. Yasui; M. Yamada: "H1 horizontal cells of carp retina have different postsynaptic mechanisms to mediate short-versus long-wavelength visual signals." Experimental Brain Research. 74. 256-262 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] S. Yasui; M. Yamada: "Analysis of non-spiking visual neurons by means of "voltage clamp by light"" Proceeding of the international Union of Physiological Sciences. XVI. 421-421 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] S. Yasui; M. Yamada: ""Voltage clamp by light" reveals a novel membrane property of the crayfish photoreceptor cell" Neuroscience Research. Suppl. 3. 53 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] M. Yamada; S. Yasui: "Instrument for membrane physiology using voltage clamp by light" Japan Soc. ME & BE. 24(specialissue). 503 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] S. Yasui; M. Yamada: "Conductance-decreasing neurotransmitter action on H1 horizontal cells in the carp retina" Neuroscience Research. Suppl. 5. 186 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] M. Yamada; S. Yasui; M. Shigematsu: "Blue / green - and red-sensitive cones make different synapses on L-type horizontal cells in the carp retina" 64th annual meeting preprint, Physiological Society of Japan. 320 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] M. Yamada; M. Fujimoto; S. Yasui: "Membrane resistance of crayfish photoreceptors re-increases gradually during light adaptation under "voltage clamp by light"" Journal of the Physiological Society of Japan. 50. 499-499 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] S. Yasui; M. Yamada; M.B.A. Djamgoz: "Effects of dopamine and APB on spectral responses of L-type horizontal cells (in Japanese)" 66th annual meeting preprint, Physiological Society of Japan. 242 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] M. Yamada; S. Yasui; M.B.A. Djamgoz: "Wavelength-dependent effects of dopamine and APB on the response of H1 horizontal cells in carp retina" Proceedings of the International Congress Physiological Sciences. XXXI. (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] S. Yasui; M. Yamada; M.B.A. Djamgoz: "Dopamine and 2-Amino-4-Phosphonobutyrate differently affect spectral responses of H1 horizontal cells in carp retina"

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] S. Yasui; M. Yamada: "Membrane resistance of crayfish retinula cells increases slowly during light adaptation under voltage clamp by light"

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] S.Yasui;M.Yamada: Experimental Brain Research. 74. 256-262 (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] M.Yamada;S.Yasui: Journal of Neuroscience Methods. 24. 65-72 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 安井湘三,山田雅弘: 電子情報通信学会技術報告. MBE87ー119. 131-134 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] S.Yasui: Biomedical Research. 9(suppl.2). 119-123 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] M.Yamada;M.Fujimoto;S.Yasui: Journal of the Physiological Society of Japan. 50. 499 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] S.Yasui;M.Yamada;B.A.Djamgoz: Proc.Natl.Acal.USA.

    • Related Report
      1988 Annual Research Report
  • [Publications] Syozo Yasui: Neuroscience Research. (1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] 安井湘三: 電子通信学会技術報告(MEとバイオサイバネティックス). (1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] Syozo Yasui: Proceedings of the International Union of Physiological Sciences(abstract). 【XVI】. 564 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Syozo Yasui: Neuroscience Research(abstract). (1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] 安井湘三,山田雅弘,重松征史: 第64回日本生理学会大会予稿集(要綱). 217 (1987)

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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