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Transport of Phosphoenolpyruvate across the Biological Membrane

Research Project

Project/Area Number 62045042
Research Category

Grant-in-Aid for Overseas Scientific Survey.

Allocation TypeSingle-year Grants
SectionUniversity-to-University Cooperative Research
Research InstitutionFukuoka University

Principal Investigator

HAMASAKI Naotaka  Fukuoka University School of Medicine, Fukuoka, Japan, 医学部, 教授 (00091265)

浜崎 直孝 (1989)  福岡大, 医, 教授

濱崎 直考 (1987)  福岡大, 医, 助教授

Co-Investigator(Kenkyū-buntansha) RICHARD J.La  米国イリノイ大学, 医学部, 助教授
OMACHI Akira  University of Illinois at Chicago, Health Sciences Center, U.S.A., 医学部, 教授
OKUBO Kenshi  Fukuoka University School of Medicine, Fukuoka, Japan, 医学部, 助手 (40194097)
LABOTKA Lichard J.  University of Illinois at Chicago, Health Sciences Center, U.S.A.
RICHARD J La  イリノイ大学, 医学部, 助教授
Project Period (FY) 1987 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥4,300,000 (Direct Cost: ¥4,300,000)
Fiscal Year 1989: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1988: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1987: ¥1,600,000 (Direct Cost: ¥1,600,000)
KeywordsPhosphoenolpyruvate transport across the cell membrane / Red Cell Membrane / Band 3 / Phosphate transport system / Histidine residue / Mediated transport / Structure of transmembrane protein
Research Abstract

This project is concerned with the transport mechanisms of phosphoenolpyruvate across the red cell membrane. Results of this project are summarized as follows: 1. Transport of phosphoenolpyruvate is mediated by Band 3 protein of red cell membrane as well as the transports of chloride and phosphate. 2. The 60th-lysine residue from the COOH-terminal end of Band 3 constitutes at least a part of the active center of the transport system. 3. In addition to the lysine residue, a histidine residue within Band 3 participates in the transport system. The histidine is located at the intracellular surface of the cell membrane and allosterically interacts with the transfer site. 4. Phosphoenolpyruvate transports across not only the red cell membrane but also the cell membranes of kidney and liver.

Report

(2 results)
  • 1989 Final Research Report Summary
  • 1987 Final Research Report Summary
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] Youichi Kawano: "Localization of the Pyridoxal Phosphate Binding Site at the COOH-terminal Region of Erythrocyte Band3 Protein" J.Biological Chemistry. 263. 8232-8238 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kenji Izuhara: "Conformational Change of Band3 Protein Induced by Diethylpyrocarbonate Modification in Human Erythrocyte Ghosts" Biochemistry. 28. 4725-4728 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Naotaka Hamasaki: "Anion Transport Center of Band3 in Erythrocyte Membranes" Studia Biophysica. 134. 127-131 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Naotaka Hamasaki: "Inhibition of Chloride Binding to the Anion Transport Site by Diethylpyrocarbonate Modification of Band3" J.Membrane Biology.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Naotaka Hamasaki: "Anion Transport Protein of the Red Blood Cell Membrane" Elsevier Science Publishers, 230 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Y. Kawano, K. Okubo, F. Tokunaga, T. Miyata, S. Iwanaga and N. Hamasaki: "Localization of the Pyridoxal Phosphate Binding Site at the COOH-terminal Region of Erythrocyte Band 3 Protein." J. Biol. Chem.263. 8232-8238 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] K. Izuhara, K. Okubo and N. Hamasaki: "Conformational Change of Band 3 Protein Induced by Diethyl Pyrocarbonate Modification in Human Erythrocyte Ghosts." Biochemistry. 28. 4725-4728 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] N. Hamasaki, K. Izuhara, K. Okubo, D. Kang and A. Omachi: "Anion Transport Center of Band 3 in Erythrocyte Membranes." Studia Biophysica. 134. 127-131 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] N. Hamasaki, K. Izuhara, K. Okubo, Y. Kanazawa, A. Omachi and R. A. Kleps: "Inhibition of Chloride Binding to the Anion Transport Site by Diethyl-pyrocarbonate Modification of Band 3." J. Membrane Biol. (in press). (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Naotaka Hamasaki and Michael L. Jennings: Anion Transport Protein of the Red Blood Cell Membrane. Elsevier Science Publishers B.V., 1-230 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Eur,J,Biochem,. 132巻,3号. (1983)

    • Related Report
      1987 Final Research Report Summary
  • [Publications] J.Biol.Chem.258巻,9号. (1983)

    • Related Report
      1987 Final Research Report Summary
  • [Publications] J.Biol.Chem.258巻,24号. (1983)

    • Related Report
      1987 Final Research Report Summary
  • [Publications] Biochem.Biophys.Res.Commun.122巻,2号. (1984)

    • Related Report
      1987 Final Research Report Summary
  • [Publications] J.Biochem.99巻,2号. (1986)

    • Related Report
      1987 Final Research Report Summary
  • [Publications] J.Biochem.100巻,1号. (1986)

    • Related Report
      1987 Final Research Report Summary
  • [Publications] Biomed.Biophys.Acta. 46巻,2/3号. (1987)

    • Related Report
      1987 Final Research Report Summary
  • [Publications] J.Biol.Chem.262巻,13号. (1987)

    • Related Report
      1987 Final Research Report Summary
  • [Publications] Trends in Biochem.Sci.(TIBS). 12巻,5号. (1987)

    • Related Report
      1987 Final Research Report Summary
  • [Publications] Trends in Biochem.Sci.(TIBS). 12巻,5号. (1987)J.Biol.Chem.

    • Related Report
      1987 Final Research Report Summary
  • [Publications] Trends in Biochem.Sci.(TIBS). 12巻,5号. (1987)J.Biol.Chem.Biochem.Biophys.Res.Commun.

    • Related Report
      1987 Final Research Report Summary

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Published: 1987-04-01   Modified: 2016-04-21  

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