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Cell biology on physiological functions of the cytoskeleton.

Research Project

Project/Area Number 62300004
Research Category

Grant-in-Aid for Co-operative Research (A)

Allocation TypeSingle-year Grants
Research Field 広領域
Research InstitutionGunma University

Principal Investigator

ISHIKAWA Harunori  Gunma Univ. School of Med., Anat., Prof., 医学部, 教授 (90010058)

Co-Investigator(Kenkyū-buntansha) IZUTSU Kosaku  Mie Univ. School of Med., Pathol., Prof., 医学部, 教授 (50024617)
WATANABE Yoshio  Tsukuba Univ. Inst. of Biol. Sci., Biol., Prof., 生物科学系, 教授 (00015918)
MOHRI Hideo  Univ. of Tokyo Coll. of Atrs & Sci., Biol., Prof., 教養学部, 教授 (70012268)
SATO Hidemi  Nagoya Univ. Fac. of Sci., Cell Biol., Prof., 理学部, 教授 (40109260)
HIRAMOTO Yukio  Univ. of the Air. Biol., Prof., 教授 (50011440)
広川 信隆  東京大学, 医学部, 教授 (20010085)
宝谷 紘一  京都大学, 理学部, 助教授 (80025444)
柴岡 弘郎  大阪大学, 理学部, 教授 (60087054)
秦野 節司  名古屋大学, 理学部, 教授 (50022570)
祖父江 憲治  大阪大学, 医学部, 教授 (20112047)
佐藤 周子  愛知県がんセンター, 研究所, 部長 (30073125)
Project Period (FY) 1987 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥17,400,000 (Direct Cost: ¥17,400,000)
Fiscal Year 1989: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1988: ¥5,600,000 (Direct Cost: ¥5,600,000)
Fiscal Year 1987: ¥9,300,000 (Direct Cost: ¥9,300,000)
KeywordsCytoskeleton / Mitosis / Cell Motility / Neuron / Actin / Tubulin / Plasmalemmal Undercoat / Cell Adhesion / チューブリン / 中間径フィラメント / 細胞膜裏打ち構造
Research Abstract

The present cooperative study was made by 23 researchers from different laboratories to elucidate the physiological functions of the cytoskeleton, particularly its regulatory roles in various cellular functions. To achieve this project we set five major subjects with emphasis on the promotion of cooperative or joint researches among the members. In fact, such collaborated works were successfully made in many aspects of our researches. The results obtained are as follows.
(1) Roles of cytoskeleton in mitosis: Using various cells such as sea urchin eggs, cultured cells and plant cells, the ultrastructure, molecular organization, dynamics and drug sensitivity of mitbtic apparatus or spindles were examined to elucidate the roles of cytoskeletal components in chromosome movement and cytokinesis.
(2) Cytoskeletal involvement in neuronal functions: The three-dimensional architecture of the cytoskeletal system in axons, dendrites and synapses were visualized, and the localization, transport, mol … More ecular structures and intramolecular functional domains of cytoskeletal proteins were also demonstrated. Microinjection of labeled molecules into culls also provided fruitful informations on dynamic aspects of cytoskeletal proteins.
(3) Cytoskeletal components in cell motility: Actin and actin binding proteins were isolated from Physarum, Tetrahymena, sea urchin eggs. A new contractile structure was examined in isolated Heliozoan axopodia. Dynamic features of actin and tubulin during cellularization in Drosophila embryos were successfully analyzed.
(4) Molecular functions of cytoskeletal proteins: Functional domains within a tubulin molecule were identified. Monoclonal antibody staining showed the specific composition of tubulin isoforms within mitotic spindles. Regulatory roles of MAPs in assembly and disassebly of microtubules were demonstrated emphasizing dynamic instability.
(5) Functional significances of the plasmalemmal undercoat: Adherence junctions were successfully isolated and characterized to elucidate the molecular organization of the junctions. Oncogene products (tyrosinekinases) were shown to be concentrated in the junctions. Significances of the plasmalemmal undercoat were proposed as the cytoskeleton supporting the plasmalema. Less

Report

(4 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • 1987 Annual Research Report
  • Research Products

    (37 results)

All Other

All Publications (37 results)

  • [Publications] Ishikawa,H.: "Plasmalemmal undercoat:The cytoskeleton supporting the plasmalemma." Arch.Histol.Cytol.51. 127-145 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Hiramoto,Y.& Nakano,Y.: "Micromanipulation studies of the mitotic apparatus in sand dollar eggs." Cell Motil.Cytoskel.10. 172-184 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Sato,H.,Kobayashi,A.,& Itoh,J.T.: "Molecular basis of physical and chemical probes for spindle assembly." cell Struct.Funct.14. 1-34 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Mohri,H.& Hosoya,N.: "Two decades since the naming of tubulin-The multi-facets of tubulin-." Zool.Sci.5. 1165-1185 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Hirono,.,Kumagai,Y.,Numata,O.& Watanabe,Y.: "Purification of Tetrahymena actin reveals some unusual properties." Proc.Natl.Acad.Sci.USA. 86. 75-79 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Izutsu,K.: "Changes of chromosomal spindle(kinetochore)fibers and the behavious of bivalent chromosomes in grasshopper spermatocytes after irradiation of the kinetoshores with an ultraviolet microbeam." Protoplama Suppl.1. Suppl.1. 122-132 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ishikawa, H.: "Plasmalemmal undercoat: The cytoskeleton supporting the plasmalemma." Arch. Histol. Cytol.51. 127-145 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Hiramoto, Y. & Nakano, Y.: "Micromanipulation studies of the mitotic apparatus in sand dollar eggs." Cell Motil. Cytoskel., 10: 172-184 (1988).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Sato, H., Kobayashi, A. & Itoh, J.T.: "Molecular basis of physical and chemical probes for spindle assembly." Cell Struct. Funct., 14: 1-34 (1989).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Mohri, H. & Hosoya, N.: "Two decades since the naming of tubulin - The multi-facets of tubulin." Zool. Sci., 5: 1165-1185 (1988).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Watanabe, Y., Ohba, H., Hirono, M. & Yasuda, T.: "Analysis of furrow formation and furrowing during cell division in Tetrahymena using cell-division-arrest (cda) mutants." Ann. New York Acad. Sci. (In press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Izutsu, K.: "Changes of chromosomal spindle (kinetochore) fibers and the behavior of bivalent chromosomes in grasshopper spermatocytes after irradiation of the kinetochores with an ultraviolet microbeam." Protoplasma Suppl. 1: 122-132 (1988).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Furuhashi, K. & Hatano, S.: "A fragmin-like protein from plasmodium of Physarum polycephalum that severs F-actin and caps the barbed end of F-actin in a Ca^<2+>-sensitive way." J. Biochem., 106: 311-318 (1989).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Shigenaka, Y., Yano, K. & Imada, M.: "Axopodial contraction evoked by electrical stimulation and its ultrastructural analysis in a heliozoan Echinospherium." Zool. Sci., 6: 45-53 (1989).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Yamao, W. & Miki-Noumura, T.: "Effect of hexyleneglycol on meiotic division of starfish oocytes." Zool. Sci., 5: 563-572 (1988).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kakimoto, T. & Shibaoka, H.: "Cytoskeletal ultrastructure of phragmoplast-nuclei complexes isolated from cultured tobacco cells." Protoplasma Suppl. 2: 95-103 (1988).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Mitsuzaki, F., Matsumoto, S. & Yahara, I.: "Truncation of the carboxyy-terminal domain of yeast b-tubulin causes temperature-sensitive growth and hypersensitivity to antimitotic drugs." J. Cell Biol., 107: 1427-1435 (1988).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Hotani, H. & Horio, T.: "Dynamics of microtubules visualized by darkfield microscopy: Treadmilling and dynamic instability." Cell Motil. Cytoskel., 10: 229-236 (1988).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Mabuchi, I. & Kane, R.E.: "A 250K-molecular-weight actin-binding protein from actin-based gels formed in sea urchin egg cytoplasmic extract." J. Biochem., 102: 947-956 (1987).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ishikawa,H.: "Plasmalemmal undercoat:The cytoskeleton supporting the Plasma lemma." Arch.Histol.Cytol.51. 127-145 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] Hiramoto,Y.& Nakano,Y.: "Micromanipulation studies of the mitotic apparatus in sand dollar eggs." Cell Motil.Cytoskel.10. 172-184 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] Sato,H.,Kobayashi,A.,& Itoh,J.T.: "Molecular basis of physical and chemical probes for spindle assembly." Cell Struct.Funct.14. 1-34 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Mohri,H.& Hosoya,N.: "Two decades since the naming of tubulin-the multi-facets of tubulin-" Zool.Sci.5. 1165-1185 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] Hirono,M.,Kumagai,Y.,Numata,O.& Watanabe,Y.: "Purification of Tetrahymena actin rerveals some unusual properties." Proc.Natl.Acad.Sci.USA. 86. 75-79 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Izutsu,K.: "Changes of chromosomal spindle(kinetochore)fibers and the behaviour of bivalent chromosomes in grasshopper spermatorytes after irradiation of the kinetochores with an ultraviolet microbeam." Protoplasma Suppl.1. Suppl.1. 122-132 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] 石川春律: Arch.Histol.Cytol.51. 127-145 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 佐藤英美: Cell struct.Funct.14. 1-34 (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] 秦野節司: Cell Motil.Cytoskel.10. 410-419 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 柴岡弘郎: Protoplasma Suppl.2. 95-103 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 宝谷紘一: Cell Motil.Cytoskel.10. 229-236 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 広川信隆: J.Molec.Biol.202. 297-305 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 石川春律: Arch.Histol.Cytol.51. (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] 佐藤英美: Critical Review of Anatomy. (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] 秦野節司: Fortschritte der Zoologie. 34. 41-52 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 佐藤周子: Nature. 328. 649-652 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 広川信隆: J.Cell Biol.106. (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] L.J.Anghileri,ed.;祖父江 憲治: The Role of Calcium in Physiological system. 4. 21-35 (1987)

    • Related Report
      1987 Annual Research Report

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Published: 1987-04-01   Modified: 2016-04-21  

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