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高等動物の動く遺伝子とそれを利用した遺伝子発現・調節制御系の分子生物学的研究

Research Project

Project/Area Number 62304001
Research Category

Grant-in-Aid for Co-operative Research (A)

Allocation TypeSingle-year Grants
Research Field 遺伝学
Research InstitutionOchanomizu University

Principal Investigator

石和 貞男  お茶の水女子大学, 理学部, 教授 (20017205)

Co-Investigator(Kenkyū-buntansha) 西田 育巧  愛知県がんセンター研究所, 分子生物学, 室長 (50107059)
向井 輝美  九州大学, 理学部, 教授 (30091242)
堀 浩  北海道大学, 理学部, 教授 (40000814)
西郷 薫  東京大学, 理学部, 教授 (50136454)
堀田 凱樹  東京大学, 理学部, 教授 (30010036)
Project Period (FY) 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥26,500,000 (Direct Cost: ¥26,500,000)
Fiscal Year 1989: ¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 1988: ¥11,100,000 (Direct Cost: ¥11,100,000)
Fiscal Year 1987: ¥11,700,000 (Direct Cost: ¥11,700,000)
Keywords動く遺伝子 / P因子 / コピア / G6PD / 光受容細胞 / ホメオボックス遺伝子 / raf情報伝達径路 / 神経細胞分裂調節 / ショウジョウバエ / レトロポゾン / 神経細胞 / 形態形成 / 癌遺伝子 / キイロショウジョウバエ / レトロウイルス / 遺伝子導入法 / 培養細胞 / 遺伝子発現調節 / 発生異常突然変異体
Research Abstract

A.動く遺伝子の構造と機能:転移を誘発されたP因子をもつ染色体のDNA解析からPの転移が複製型であることを明らかにした。ゲルシフト法からトランスポゼ-スの結合する部位はPの両末端から200bp以内にあること、kp因子をゲノム中にもつM´系統の中にはP因子の転移を強く抑制することなども分かった。一方、レトロトランスポゾンの一つであるジョッキ-因子の分布はキイロショウジョウバエの近縁4種に限られていた。この因子の挿入率は1.54×10^<-4>/II染色体/世代と推定された。又、5kbコピアRNAから2kbRNAへのスプライシングがウィルス様粒子(VLP)形成に必要であることが明らかとなった。
B.動く遺伝子と遺伝子発現:G6PD遺伝子の上流に挿入している欠損型P因子は、この遺伝子の発現に正の調節作用をする。今後、このP因子に結合すると予想される核蛋白の同定と調節作用に関する研究が期待される。挿入されたP因子の特徴(長さ・向き・位置)とsn遺伝子の発現との関係が明らかになった。
C.新たに見出された遺伝子の分子生物学:複眼形態形成に関する突然異変体から、光受容細胞に特異的発現をするホメオボックス遺伝子を同定した。光受容細胞の遂次的分化の研究へと発展させたい。ショウジョウバエによるがん遺伝子研究の一端としてraf変異を抑制する突然変異を誘発・分離,raf情報伝達径路の解明を試みている。初期胚両端部での分化決定に関わる情報伝達径路をtsl→tor→raf→su(1)raf-1→tllと現在は考えている。細胞の寿命と関係すると思われるス-パ-オキシドデスムタ-ゼの遺伝子をマウスのデ-タを参考にしてショウジョウバエから単離目下塩基配列決定を進めている。P因子挿入突然変異体の一つから、神経細胞分裂調節に働いている遺伝子の分離に成功した。この遺伝子は発生時期特異的な調節を受けているのでその機構に注目した研究を行う。

Report

(3 results)
  • 1989 Annual Research Report
  • 1988 Annual Research Report
  • 1987 Annual Research Report
  • Research Products

    (47 results)

All Other

All Publications (47 results)

  • [Publications] Okuyama,Eisaku: "Nucleotide sequences of the duplicated amylase structural genes in Drosophila melanogaster:one active and one null strain." Proc.Jpn.Acad.64. 274-277 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] Shiokawa,Koichiro: "Persistnece and expression of circular DNAs encoding Drosophila amylase,bacterial chloramphenicol acetyltransferase,and others in Xenopus." Cell Structure and Function. 14. 261-269 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Matsuo,Yoshinori: "t-RNA derived insertion element in histone gene repeating unit of Drosophila melanogaster." Nucleic Acid Res.17. 225-238 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Matsuo,Yoshinori: "Nucleotide variation and divergence in the histone multigene family in Drosophila melanogaster." Genetics. 122. 87-97 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Y.Nishida,M.Hata,T.Ayaki,H.Ryo,M.Yamagata,K.Shimizu,Y.Nishizuka: "Proliferation of both somatic and germ cells is affected in the Drosophila mutants of raf proto-oncogene" The EMBO Journal. 7. 775-781 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] M.Yamaguchi,Y.Nishida,T.Moriuchi,F.Hirose,C.-C.Hui,Y.Suzuki,A.Matsukage: "Drosophila proliferating cell nuclear antigen(cyclin)gene:structre,expression during development,and specific binding of homeodomain proteins to its 5´-flanking region" Molecular and Cellular Biology. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Shi-ichi Higashijima: "Photoreceptor-typeーspecific expression of BarH1,a Drosophila homeobox gene causing Bar mutation" Nature. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Harada,K.: Proc. Jpn. Acad.64. 163-166 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Inoue,H.: J.Biochem.103. 91-94 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Itoh.M.: Genet. Res.52. 169-177 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Itoh.M.: Mol. Cell Biol.

    • Related Report
      1988 Annual Research Report
  • [Publications] Kanda,T.: Nucl. Acids Res.1988. (157-160)

    • Related Report
      1988 Annual Research Report
  • [Publications] Koga,A.: Genetics. 121.

    • Related Report
      1988 Annual Research Report
  • [Publications] 堀田凱樹、岡田益吉: シリーズ 分子生物学の進歩9 ショウジョウバエの発生遺伝学. 1989.

    • Related Report
      1988 Annual Research Report
  • [Publications] Miyake,T.: Invertebrete and fish tissue culture. Production of retrovirus-like particles by transposons in genome of Drosophila simulans.1988. (115-118)

    • Related Report
      1988 Annual Research Report
  • [Publications] Langley,C.H.: Genetics.119. 619-629 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Matsumoto,E.: Cell Tissue Res.252. 293-300 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Matsuo,Y.: Nuc. Acids Res.17. 225-238 (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Nishida,Y.: EMBO J.7. 775-781 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Nitasaka,E.: Jpn. J. Genet.63. 303-312 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Okuyama,E.: Proc. Jpn. Acad.64. 274-277 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Sakai,T.: Cell Diff. & Devel.

    • Related Report
      1988 Annual Research Report
  • [Publications] Sato,T.: RouxS^^1 Archieves Devel. Biol.197. 435-440 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Shiokawa,K.: Cell Struct. Func.

    • Related Report
      1988 Annual Research Report
  • [Publications] Tanaka,Y.: J.Biochem.104. 489-491 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Tanda,S.: Mol. Gen. Genet.214. 405-411 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Ui,K.: Jpn. J. Genet.63. 33-41 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Yamada,T.: Biomed. Res.9. 437-442 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Yosihara,M.: Proc. Jpn. Acad. (ser.B). 64. 172-176 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 西田育巧: 組織培養. 14. 118-122 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 西田育巧: 蛋白質・核酸・酸素. 34. 43-51 (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] 佐藤隆: 遺伝. 42(4). 48-53 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Hagiwara,N.;Nakamura,E.;Matsuura,E.T.;Chigusa,S.I.: Genet.Res.50. 105-111 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Miyake,T.;Mae,N.;Shiba,T.;Kondo,S.: Mol.Gen.Genet.207. 29-37 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Saigo,K.;Kanda,T.: Nucleic Acids Reserch,Symposium Series. 19. 157-160 (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] 柴忠義: Biomedica. 10. 38-43 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Fujita,S.C.;Inoue,H.;Yoshioka,T.;Hotta,Y.: Biochem.J.243. 97-104 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Okamaoto,H.;Sakai,K.;Goto,S.;Takasu-ishikawa,E.;Hotta,Y.: Proc.Japan Acad.63. 284-288 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Inoue,H.;Yoshioka,T.;Hotta,Y.: J.Biochem.103. 91-94 (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] Nishida,Y.;Hata,M.;Ayaki,T.;Ryo,H.;Yamagata,M.;Shimizu,K.;Nishizuka,Y.: EMBO J.(1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] Nishida,Y.;Hata,M.;Nishizuka,Y.;Rutter,W.J.;Ebina,Y.: Biochem.Biophys.Res.Commun.141. 474-481 (1986)

    • Related Report
      1987 Annual Research Report
  • [Publications] Koga,A.;Kusakabe,S.;Tajima,F.;Harada,K.;Bewley,G.C.;Mukai,T.: Proc.Japan Acad.64. 9-12 (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] Itoh,M.Iwabuchi,M.;Yorimoto,N.;Hori,S.H.: Genet.Res.

    • Related Report
      1987 Annual Research Report
  • [Publications] Sato,T.;Denell,R.E.: Genetics. 116. 389-398 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Sato,T.: Jpn.J.Genet.62. 493-499 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Ueda,R.;Takahisa,M.;Togashi,S.;Maruyama,S.;Miyake,T.: Jpn.J.Genet.62. 45-50 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Miyake,T.;Mae,N.;Shiba,T.;Kondo,S.;Takahisa,M.;Ueda,R.;ed.Karl Maramorosch: "Biotechnoilogy in Invertebrane Pathology and cell culture Capter 16.Transfection of Drsophila melanogster Transposable elements into the Drosophila hydei Cell Line" Academec Press, 251-263/51 (1987)

    • Related Report
      1987 Annual Research Report

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Published: 1987-04-01   Modified: 2016-04-21  

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