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Molecular mechanisms of chilling injury and freezing injury

Research Project

Project/Area Number 62304004
Research Category

Grant-in-Aid for Co-operative Research (A)

Allocation TypeSingle-year Grants
Research Field 植物生理学
Research InstitutionHokkaido University

Principal Investigator

YOSHIDA Shizuo  The Institute of Low Temperature Science, Hokkaido Univ. Prof., 低温科学研究所, 教授 (90001651)

Co-Investigator(Kenkyū-buntansha) MATSUO Tomoaki  Dept. Agriculture, Kagoshima Univ. Res. Assoc., 農学部, 助手 (90041673)
SEKIYA Jiro  Dept. Agriculture, Okayama Univ. Assoc.Prof., 農学部, 助教授 (10035123)
MURATA Norio  Institute of Basic Biology, Prof., 基礎生物学研究所, 教授 (90011569)
SAKATA Toru  Dept. Science and Technology, Keio Univ. Prof., 理工学部, 教授 (10051020)
ITO Sisuke  Dept. Agricultural Chemistry, Obihiro Univ. Agriculture and Veterinary Medicine,, 畜産学部, 教授 (30003099)
菅原 康剛  埼玉大学, 理工学部, 助教授 (70114212)
Project Period (FY) 1987 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥17,500,000 (Direct Cost: ¥17,500,000)
Fiscal Year 1989: ¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 1988: ¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 1987: ¥8,000,000 (Direct Cost: ¥8,000,000)
KeywordsMechanisms of chilling injury / Mechanisms of freezing injury / Phase transition of membrane lipids / Chilling injury and tonoplasts / Low temperature and tonoplast proton pumps / Glycerol-3-phosphate acyltransferase / Purification of enzymes and cDNA cloning / Tonoplast H^+-ATPase and H^+-PPase / 飽和極性脂質分子種 / 液胞膜プロトン輸送の温度依存性 / 低温慣化の機構と細胞膜 / 低温傷害 / 凍結傷害 / 飽和脂質分子種 / 液胞とH^+-ATPase / スフィンゴ糖脂質 / 電子顕微鏡 / ^<31>P-NMR / 相転移 / ^<31>PーNMR / H^+ーATPase / スフィンゴ脂質 / 脂質合成酵素
Research Abstract

Experimental results obtained through this Research Project are summarized as follows: 1) Molecular mechanisms of chilling injury. Following two factors were determined to be primary important for chilling injury in plant cells. First, a) a dysfunction of chloroplasts due to a low temperature-induced phase separation of disaturated molecular species of phosphatidylglycerol in the lipid bilayers. The correlation between the content of disaturated phosphatidylglycerol and the chilling sensitivity of plants was established both in hevaceous and woody plant species. The phase separation of disaturated phosphatoidylglycerol in intact membrane systems under low temperatures was determined by an analysis of msec delayed fluorescence.The key enzyme which controls the synthesis of the disaturated molecular species of phosphatidylglycerol was purified from chilling sensitive squash cotyledons and the CDNA was successfully cloned. Second, b) a disturbance of an iconic environment of cytoplasms du … More e to a low temperature-induced dysfunction of tonoplast proton transports. In mung bean cells, the tonoplast H^+-ATPase is impaired by a relatively short time of chilling in vivo, giving rise to an acidification of the cytoplasms. Two types of the proton transporting enzymes, i.e., H^+-ATPase and H^+-PPase, were purified from mung bean seedlings and characterized. The low temperature-induced inactivation of the H^+-ATPase is found to be related to a specific release of 57, 68 and 32 kDa subunits. 2) Mechanisms of freezing injury. Based on electronmicroscopic studies, it was confirmed that a freeze-induced ultrastructural changes in plasma membranes is primary important factor for cell injury. Upon a lethal freezing of cells, an aggegation of intramebranous particles and a formation of particle-free area, and a formation of Hexagonal THY phase structure were observed in plasma membranes specifically related to chill injury. However, these ultrastructural changes seemed to be dependent on plant species and the cell types, suggesting a diversity in the mechanisms of freezing injury. In sold acclimation of plants, a molecular rearrangement of plasma membranes, which includes qualitative changes in membrane proteins and enrichment of unsaturated phospholipids, is considered to play an important role in stabilization of plasma membranes against freezing stress.
These results may provide a basis for molecularbiological approaches to the mechanisms of chilling injury and freezing injury in plants in future. Less

Report

(4 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • 1987 Annual Research Report
  • Research Products

    (50 results)

All Other

All Publications (50 results)

  • [Publications] C.Matsuura-Endo,M.Maeshima and S.Yoshida: "Subunit composition of vacuolar membrane H^+-ATPase from mung bean." Eur.J.Biochem.187. 745-751 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] M.Maeshima and S.Yoshida: "Purification and properties of vacuolar membrane proton translocating inorganic pyrophosphatase from mung bean." J.Biol.Chem.264. 20068-20073 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] O.Ishizaki,I.Nishida,K.Agata,G.Eguchi and N.Murata: "Cloning and nucleotide sequence of cDNA from the plastid glycerol-3-phosphate acyltransferase from squash" FEBS Lett.238. 424-430 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Y.Tasaka,I.Nishida,S.Higashi,T.Beppu and N.Murata: "Fatty acid composition of phospphatidylglycerols in relation to chilling sensitivity of woody plants." Plant.Cell Physiol.(in press). (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] D.C.Fork and N.Murata: "The effect of light intensitiy on the assay of the low temeprature limit of photosynthesis using msec delayed light emission." Photosynthesis Res.(in press). (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] J.Sekiya: "Molecular species of phosphatidylglycerol from leaves of various Citrus species." Agr.Biol.Chem.(in press). 54. (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] S.Yoshida and M.Uemura: "Responses of the plasma membrane to cold acclimation and freezing stress.In the Plant Plasma Membrane-Structure,Fuction and Molecular Bi-logy(C.Larsson,M.Moller eds)" Springer Verlag, inpress (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] N.Murata and I.Nishida: "Lipids in relation to chilling sensitivities of plants In Chilling Injury of Horticultural Crops(C.Y.Wang eds)" CRC Press, inpress (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Yoshida, S., K.Washio, J.Kenrick and G.Orr: "Thermotropic properties of lipids extracted from plasma membrane and tonoplast isolated from mung bean (Vigna radiata L. Wilczek)" Plant Cell Physiol.29. 1411-1416 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kawata, T.and S.Yoshida: "Alterations in protein synthesis in vivo in chilling sensitive mung bean hypocotyls caused by chilling stress" Plant Cell Physiol.29. 1423-1427 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Yoshida, S., C.Matsuura and S.Etani: "Impairment of tonoplast H^+-ATPase as an initial physiological response of cells to chilling in mung bean (Vigna radiata L. Wilczek)." Plant Physiol.89. 634-642 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Maeshima, M.and S.Yoshida: "Purification and properties of vacuolar proton-translocating inorganic pyrophosphatase from mung bean" J.Biol.Chem.264. 20068-20073 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Matsuura-Endo, C., M.Maeshima, and S.Yoshida: "Subunit composition of vacuolar membrane H^+-ATPase from mung bean." Eur.J.Biochem.187. 745-751 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ishizaki, O., I.Nishida, K.Agata, G.Eguchi and N.Murata: "Cloning and nucleotide sequence of cDNA for the plastid glycerol-3-phosphate acyl transferase from squash." FEBS Lett.238. 424-430 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Tasaka, Y., I.Nishida, S.Higashi, T.Beppu and N.Murata: "Fatty acid composition of phosphatidylglycerol in relation to chilling sensitivities of woody plants." Plant Cell Physiol. 1990.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Fork, D.C., and N.Murata: "The effect of light intensity on the low temperature limit of photosynthesis using mSec delayed light emission." Photosynthetic Res 1990.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Sekiya.J., K.Nagai and N.Shimose: "Molecular species of phosphatidylglycerol from leaves of various Citrus species." Agr.Biol.Chem. 54:1990.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kasamo, K.: "Response of tonoplast and plasma membrane ATPase in chilling sensitive and insensitive Rice (Oryza sativa L.)." Plant Cell Physiol.29. 1085-1089 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Fujikawa, S.: "Chemica, l fixation-induced particle aggregation in plasma membranes of chilling injured plant cells." Cryo-Letters. 9. 338-347 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Fujikawa, S.: "Artificial biological membrane ultrastructural changes caused by freezing." Electron Microsco.Rev.1. 113-140

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ohnishi, M., H.Imai, M.Kojima S.Yoshida, N.Murata, Y.Fujino and S.Ito: "Separation of cerebroside species in plants by reversed-phase HPLC and their phase transition temperatures." Proceedings of Session Lectures and Scientific Presentations on ISF-JOCS World Congress. Vol.II. 930-935

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Yoshida, S., and M.Uemura: Responses of the plasma membrane to cold acclimation and freezing stress. In The Plasma Membrane (C.Larsson and M.Moller eds). Springer Verlag., (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Murata, N., and I.Nishida: Lipids in relation to chilling sensitivities of plants. In Chilling Injury of Horticultural Crops (C.Y.Wang ed). CRC Press, (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] C.Matsuura-Endo,M.Maeshima and S.Yoshida: "subunit composition of vacuolar membrane H^+-ATPase from mung bean." Eur.J.Biochem.187. 745-751 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] M.Maeshima and S.Yoshia: "Purification and properties of vacular memebrane proton translocating inorganic pyrophosphatase from mung bean." J.Biol.Chem.264. 20068-20073 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] O.Ishizaki,I.Nishida,K.Agata,G.Eguchi and n.Murata: "Cloning and nucleotide sequence of cDNA from the plastid glycerol-3-phosphate acyltransferase form squash" FEBS Lett.238. 424-430 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] Y.Tasaka, I.Nishida,S.Higashi,T.Beppu and N.Murata: "Fatty acid composition of phospphatidylglycerols in relation to chilling sensitivity of woody plants." Plant.Cell Physiol.(1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] D.C.Fork and N.Murata: "The effect of light intensitiy on the assay of the low temeprature limit of photosynthesis using msec delayed light emission." Photosynthesis Res.(1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] J.Sekiya: "Molecular species of phosphatidylglycerol from leaves of various Citrus species." Agr.Biol.chem.54. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] S.Yoshida and M.Uemura: "Responses of the plasma membrane to cold acclimation and freezing stress.In The Plant plasma Membrane-Structure,fuction and Molecular Bi-logy(C.Larsson,M.Moller eds)" Springer Verlag, (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] N.Murata and I.Nishida: "Lipids in relation to chling sesitivities of plants In Chilling Injury of Horticultural Crops(C.Y.Wang eds)" CRC Press, (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Etani,S.,;Yoshida,S.: Plant Cell Physiol.28. 83-91 (1987)

    • Related Report
      1988 Annual Research Report
  • [Publications] Yoshida,S.,;Kawata,T.: Plant Cell Physiol.29. 1391-1397 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Kawata,T.,;Yoshida,S.: Plant Cell Physiol.29. 1399-1410 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Yoshida,S.,;Washio,K.,;Kenrick,J.,;Glenda,O.: Plant Cell Physiol.29. 1411-1416 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Kawata,T.,;Yoshida,S.: Plant Cell Physiol.29. 1423-1427 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Yoshida,S.,;Mtsuura,C.,;Etani,S.: Plant Physiol.89. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Frentzen,M.,;Nishida,I.,;Murata,N.: Plant Cell Physiol.28. 1195-1201 (1987)

    • Related Report
      1988 Annual Research Report
  • [Publications] Nishida,I.,;Frentzen,M.,;Ishizaki,O.,;Murata,N.: Plant Cell Physiol.28. 1071-1079 (1987)

    • Related Report
      1988 Annual Research Report
  • [Publications] Toriyama,S-I.,;Hinata,K.,;Nishida,I.,;Murata,N.: Plant Cell Physiol.29. 615-621 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Yazaki,Y.,;Asukagawa,N.,;Ishikawa,Y.,;Ohta,E.,;Sakata,M.: Plant Cell Physiol.29. 919-924 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Fujikawa,S.: J.Electron Microsc.36. 224-227 (1987)

    • Related Report
      1988 Annual Research Report
  • [Publications] Fujikawa,S.: Electron Microsc.1. 113-140 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Sekiya,J.,;Kosiso,M.,;Morita,A.,;Hatanaka,A.: "MoTecular species compsosition of phosphatidylglycerol in leaves of camellia species and chilling sensitivity.In: The Metab olism,Structurel and Function in Plant lipids." Plenum Press, 377 (1987)

    • Related Report
      1988 Annual Research Report
  • [Publications] Yoshida,S.;Uemura,M.: "Alterration of plasma membranes related to cold acclimation of plants in:Low Temperature Stress Physiology in Crops." CRC-Press, 233 (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] S.Etani;S.Ysoshida: Plen〓 and Cell Physiol. 28. 83-91 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 菅原康剛,竹内正幸: 凍結及び乾燥研究会会誌. 33. 45-48 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] I.Nishida;M.Frentzen;O.Ishizaki;N.Murata: Plant and Cell Physiol. 28. 1071-1079 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] M.Frentzen;I.Nishide;N.Murata: Plant and Cell Physiol. 28. 1195-1201 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] P.K.Stump;J.B.Mrdd;W.D.Nes,Plenum ed.;J.Sekiya;H.Koiso;A.Morita;A.Hatanake: The metabolisin,Structure and Function of Plant Lipids. 9377- (1987)

    • Related Report
      1987 Annual Research Report

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Published: 1987-04-01   Modified: 2016-04-21  

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