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Signal transduction and cellular responses in plant infection

Research Project

Project/Area Number 62304015
Research Category

Grant-in-Aid for Co-operative Research (A)

Allocation TypeSingle-year Grants
Research Field 植物保護
Research InstitutionTottori University

Principal Investigator

KOHMOTO Keisuke Prof.  Tottori University, Faculty of Agriculture, 農学部, 教授 (80032093)

Co-Investigator(Kenkyū-buntansha) OUCHI Seiji Prof.  Kinki University, Faculty of Agriculture, 農学部, 教授 (70026433)
TANI Toshikazu Prof.  Kagawa University, Faculty of Agriculture, 農学部, 教授 (50035961)
OKU Hachiro Prof.  Okayama University, Faculty of Agriculture, 農学部, 教授 (20033144)
KUNOH Hitoshi Prof.  Mie University, Faculty of Bioresources, 生物資源学部, 教授 (20024573)
NISHIMURA Syoyo Prof.  Nagoya University, Faculty of Agriculture, 農学部, 教授 (10032070)
浅田 泰次  愛媛大学, 農学部, 教授 (80036269)
Project Period (FY) 1987 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥28,300,000 (Direct Cost: ¥28,300,000)
Fiscal Year 1988: ¥12,300,000 (Direct Cost: ¥12,300,000)
Fiscal Year 1987: ¥16,000,000 (Direct Cost: ¥16,000,000)
KeywordsHost recognition / Host-specific toxin / Phytoalexin / Elicitor / Suppressor / Pathogenicity gene / Hypersensitive cell reaction / リグニン化 / 病原性遺伝子 / 抗菌性蛋白質 / 受容性
Research Abstract

In the present co-operative research program, we investigated in-depth the recognition by host cells of signal factors released from fungal pathogens at the molecular and cellular levels, and obtained many useful results as follows;
I. Isolation and characterization of pathogen's signal factors, and their biogenetic mechanisms: AT-toxin, ACT-toxin A, a host-recognition factor and an infection-inducing factor were isolated respectively from spore-germinated fluids of the brown spot pathogen of tobacco, the brown spot pathogen of Dancy tangerine, the gray mold of scallion and the rice blast fungus. Of them, the factor from the gray mold was very unique in respect to an ability to induce accessibility without causing necrosis in host cells. An epoxyradical-attached decatrienoic acid alcohol was found to be a major intermediate metabolite in the biosynthesis of AK-toxin and AF-toxin by Alternaria pathogens. Genomic library and an efficient transformation system of A. alternata Japanese pear … More pathotype were established to identify and isolate the pathogenicity gene. Receptors for signal toxins were detected in the plasma membrane of host cells. Gene for -1,3-endoglucanase that releases a phytoalexin-elicitor from Phytophthora's cell wall was cloned and its base sequence was completely determined.
II. Signal transduction and its regulation: Necrotic process in apple leaves caused by AM-toxin was found to contain a photo-inhibition phase; 3hr-dark period was necessary to complete the necrosis. A suppressor from Mycospherella antagonized the elicitor-inducing phytoalexin synthesis in pea tissues. superoxideproducing system and phospholipase activation related to hypersensitive cell reaction in potato.
III. Cellular responses to signals: Investigation of the relation between early action process of toxins whose primary targets in hosts are distinctly different and accessibility induction revealed that functional modification in plasma membranes was very colsely correlated to the induction; no necrosis was required for infection even by perthophytes. In conclusion, membrane pathology will be the key target for study of infection mechnism. Less

Report

(3 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1987 Annual Research Report
  • Research Products

    (94 results)

All Other

All Publications (94 results)

  • [Publications] Akimitsu,K.et al.: Plant Phsiology. 89. (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Doke,N.,et al: Physiol.Mole.Plent.Pathol.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Hayashi,N.,et al: Ann.Phytopathol.Soc.Japn. 54. 250-252 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kodama,M.,et al: Ann.Phytopathol.Soc.Japan.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kunoh,H.,cet al: Physiol.Molee.Plant Pathol.33. 81-93 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kunoh,H.,et al.: Experimental Mycology. 12. 325-335 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kunoh,H,et al.: Ann.Phytopatholo.Soc.Japan. 54. 577-583 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Nicholson,R.L.,et al: Experimental Mycology. 12. 336-349 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Nelson,H; T.Shiraishi et al: J.Phytopathology. (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ouchi,S.,et al: J.Phytopathology.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ouchi,S.,et al.: Phytopathology.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Park,P.et al.: Can J.Botany. 65. 330-339 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Shiraishi,et al: Physiol.Molec.Plant Pathoi.33. (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tanabe,K; Nishimura,S.et: Ann.Phytopathol.Soc.Japan. 54. 483-491 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tanabe,K.,et al: Ann.Phytopathol.Soc.Japan. 54. 525-555 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Toyoda,H.,et al.: Plant Cell Reports. 7. 293-296 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yamada,T.,et al.: Molecular Plant - Microbe Interactions.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Arase,S.,et al.: Bull.Fac.Agric.Shimane Univ.22. 176-183 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 大口富三、浅田泰次: 愛媛大学農学部紀要. 33. (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 大口富三、浅田泰次: 愛媛大学農学部紀要. 33. (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 奥八郎: "病原性とは何か" 農文協, 196 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kohmoto,K; R.D.Durbin: "Host-Specific Toxins: Recognition and Specificity Factors in Plant Disease" Tottori University,Tottori, 230 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kohmoto,K; H.Otani,et al.: "In: Phytotoxins and Plant Pathogenesis.Ed.by Graniti,A.,R.D.Durbin and A.Ballio" Springer-Verlag,Berlin, 249-2 5 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 大内成志 編: "植物病害と遺伝子工学" 朝日出版, (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tsuge,T.,H.Kobayashi and S.Nishimura: "Specific gene expression in the host-selective toxin producer,Alternaria alternata and its genetic teansformation with high efficiency In: Host-Specific Toxins: K.Kohmoto&R.D.Durb" Tottori University,Tottori, 153-170 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Arase, S. et al.: "Studies on host-selective infection mechanis of Pyricularia oryzae Cavara (1) Usefulness of rice cv. Sekiguchi-asahi as a test plant for detection of disease determinant(s)" Bull. Fac. Agric. Shimane Univ.22. 176-183 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Akimitsu, K., et al.: "Host-specific effects of toxin from the rough lemon pathotype of Alternaria alternata on mitochondrion" Plant Physiology. 89. (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Doke, N. et al.: "Physiological mechanisms of activation of O^-_ generating NADPH oxidase and phospholipase A_2 as a signransduction system in the cascade of hypersensitive reaction of potato tissues." Physiol. Molec. Plant Pathol.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Hayashi, N. et al.: "The presence of double-stranded RNAs in Alternaria alternata Japanese pear pathotype and their participation in AK-toxi productivity" Ann. Phytopathol. Soc. Japan. 54. 250-252 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kodama, M. et al.: "Host-selective AT-toxin from Alternaria isolates causing brown spot disease of tobacco" Ann. Phytopathol. Soc. Japan.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kunoh, H. et al.: "Induced accessibility and enhanced inaccessibility at the cellular level in barley coleoptiles. III. Timing and localization of enhanced inaccessibility in a single coleoptile cell and its transfer to an adjacent cell." Physiol. Molec. Plant Pathol.33. 81-93 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kunoh, H. et al.: "Induced accessibility and enhanced inaccessibility at the cellular level in barley coleoptiles. IV. Escape of the second lobe of the Erysiphe graminis appressorium from inaccessibility enhanced by the previous atatck of the first lobe." Ann. Phytopathol. Soc. Japan. 54. 577-583 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kunoh, H. et al.: "Preparation of the infection court by Erysiphe graminis. I. Contact-mediate changes in morphology of the conidium surface." Experimental Mycology. 12. 325-335 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Nicholson, R.L., et al.: "Preparation of the infection court by Erysiphe graminis. II. Release of esterase enzyme from conidia in response to a contact stimulus." Expermental Mycology. 12. 336-349 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Nelson, H., et al.: "Effect of leaf age and etiolation of barley on susceptibility to podery mildew infection." J. Phytopathology. (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ouchi, S., et al.: "Chitinase activity of barley leaves inoculated with Erysiphe graminis f. sp. hordei." Phytopathology.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ouche, S., et al.: "Susceptibility of barley to Erysiphe graminis f. sp. hordei as affected by cytokinin levels in the leaves." J. Phytopathology.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ohguchi, T., et al.: "Behavior of downew mildew fungus of Japanese radish on various media. II Culture from the infectid tissue." Mem. Coll. Agr. Ehime Univ.33. 59-69 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Park, P., et al.: "Golgi vesicles fused with invaginated plasma membranes in host cells treated with a host-specific toxin from the Japanese pear pathotype fo Alternaria alternata." Ann. Phytopathol. Soc. Japan. 54. 493-502 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Shiraishi, T., et al.: "Association of increased phenylalanine ammonia-lyase activity and cinnamic acid synthesis with the induction of temporary inaccessibility caused by Erysiphe graminis primary germ tube penetration of the barley leaf." Physiol. Molec. Plant Pathol.33. (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Toyoda, H. et al.: "A microinjection technique for conidia of Erysiphe graminis f. sp. hordei." Phytopathology. 77. 815-818 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Toyoda, H., et al.: "Intranuclear microinjection of tomato callus cells." Plant Cell Reports. 7. 293-296 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tanabe, K., et al.: "Cutinase production by Alternaria alternata Japanese pear pathotype and its role in pathogenicity" Ann. Phytopathol. Soc. Japan. 54. 483-491 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tanabe, K., et al.: "Pathogenicity of cutinase- and pectic enzymes deficient mutants of Alternaria alternata Japanese pear pathotype." Ann. Phytopathol. Soc. Japan. 54. 552-555 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yamada, T., et al.: "Effects of fungal suppressor on the host plant resistance expression." Molec. Plant - Microbe Interactions.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Mayama, S., et al.: "Selection of somaclonal variants of oats resistant to Helminthosporium victoriae which produces a host-specific toxin, victorin." Plant Tissue Culture Letters.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kohmoto, K., et al.: Host recognition: Can accessibility to fungal invasion be induced by host-specific toxins without necessitating necrotic cell death? In: Phytotoxins and Plant Pathogenesis. Graniti, A. et al. eds.Springer-Verlag, Berlin, 249-265 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kohomoto, K. & R.D. Durbin: Host-Specific Toxins: Recognition and Specificity Factors in Plant Disease. Tottori University, Tottori, 230 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ouchi, S.: Plant Diseases and Genetic Engineering (in Japanese). Asahi, Press, Tokyo, (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Nishimura, S. et al.: Phisology of Plant Infection (in Japanese). Bun-eido Press, (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Akimitsu,K.;K.Kohmoto,et al.: Plant Physiology. 89. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Doke,N.,et al.: Physiol.Molec.Plant Pathol.

    • Related Report
      1988 Annual Research Report
  • [Publications] Hayashi,N.,et al.: Ann.Phytopathol.Soc.Japan. 54. 250-252 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Kodama,M.,et al.: Ann.Phytopathol.Soc.Japan.

    • Related Report
      1988 Annual Research Report
  • [Publications] Kunoh,H.,et al.: Physiol.Molec.Plant Pathol.33. 81-93 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Kunoh,H.,et al.: Experimental Mycology. 12. 325-335 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Kunoh,H.,et al.: Ann.Phytopatholo.Soc.Japan. 54. 577-583 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Nicholson,R.L.,et al.: Experimental Mycology. 12. 336-349 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Nelson,H.,;T.Shiraishi,et al.: J.Phytopathology. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Ouchi,S.,et al.: J.Phytopathology.

    • Related Report
      1988 Annual Research Report
  • [Publications] Ouchi,S.,et al.: Phytopathology.

    • Related Report
      1988 Annual Research Report
  • [Publications] Park,P.,et al.: Can.J.Botany. 65. 330-339 (1987)

    • Related Report
      1988 Annual Research Report
  • [Publications] Shiraishi,T.,et al.: Physiol.Molec.Plant Pathol.33. (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Tanabe,K.,;Nishimura,S.,et al.: Ann.Phytopathol.Soc.Japan. 54. 483-491 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Tanabe,K.,et al.: Ann.Phytopathol.Soc.Japan. 54. 525-555 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Toyoda,H.,et al.: Plant Cell Reports. 7. 293-296 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Yamada,T.,et al.: Molecular Plant - Microbe Interactions.

    • Related Report
      1988 Annual Research Report
  • [Publications] Arase,S.,et al.: Bull.Fac.Agric.Shimane Univ.22. 176-183 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 大口富三,浅田泰次: 愛媛大学農学部紀要. 33. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] 大口富三,浅田泰次: 愛媛大学農学部紀要. 33. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Mayama,S.,et al.: Plant Tissue Culture Letters.

    • Related Report
      1988 Annual Research Report
  • [Publications] 奥八郎: "病原性とは何か" 農文協, 196 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Kohmoto,K,;R.D.Durbin: "Host-Specific Toxins:Ricognition and Specificity Factors in Plant Disease" Tottori University, 230 (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Kohmoto,K.;H.Otani,et al.: "In:Phytotoxins and Plant Pathogenesis." Springer-Verlag, 249-265 (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] 大内成志 編: "植物病害と遺伝子工学" 朝日出版, (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Tsuge,T.,;H.Kobayashi;S.Nishimura.: "Specific gene expression in the host-selective toxin producer,Alternaria alternata and its genetic transformation with high efficiency In: Host-Specific Toxins:," Tottori University, 153-170 (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Akimitsu.K.,et al.: Plant Physiology.

    • Related Report
      1987 Annual Research Report
  • [Publications] Kodama.M.,et al.: Ann.Phytopath.Soc.Japan(in preparation).

    • Related Report
      1987 Annual Research Report
  • [Publications] Arase.S.,et al.: Ann.Phytopath.Soc.Japan.

    • Related Report
      1987 Annual Research Report
  • [Publications] Neison.H.,et al.: Jour.Phytopathology.

    • Related Report
      1987 Annual Research Report
  • [Publications] Shiraishi.T.,et al.: Ann.Phytopath.Soc.Japan.

    • Related Report
      1987 Annual Research Report
  • [Publications] Kunoh.H.,et al.: Physiological and Molecular Plant Pathology.

    • Related Report
      1987 Annual Research Report
  • [Publications] 大内成志: バイオ農薬・生育調節剤開発マニュアル・ファイトアレキシン. 563 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Kunoh.H.,et al.: Experimental Mycology.

    • Related Report
      1987 Annual Research Report
  • [Publications] Nichoison,R.L.,et al.: Experimental Mycology.

    • Related Report
      1987 Annual Research Report
  • [Publications] Onoe.T.,et al.: Ann.Phytopath.Soc.Japan. 53. 210-226 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Yoshikawa.M.,et al.: Ann.Phytopath.Soc.Japan. 53. 227-241 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Yoshikawa.M.,et al.: Plant Physiology.

    • Related Report
      1987 Annual Research Report
  • [Publications] Yoshikawa.M.,et al.: Plant Physiology.

    • Related Report
      1987 Annual Research Report
  • [Publications] Toyoda.H.,et al.: Phytopathology. 77. 815-818 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Toyoda.H.,et al.: Phytopathology. 78. (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] 浅田泰次: 四国植防. 23.

    • Related Report
      1987 Annual Research Report
  • [Publications] Nishimura.S.,et al.: "Molecular Determinants of Plant Diseases" Japan Scientific Societies Press/Springer-Verlag, 293 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Kohmoto.K.,et al.: "Phytotoxins and Plant Pathogenesis" Springer-Verlag,

    • Related Report
      1987 Annual Research Report

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Published: 1987-04-01   Modified: 2016-04-21  

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