| Project/Area Number |
62304026
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| Research Category |
Grant-in-Aid for Co-operative Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
畜産学(含草地学)
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| Research Institution | Kyoto University |
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Principal Investigator |
IRITANI Akira Kyoto University, 農学部, 教授 (80026385)
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| Co-Investigator(Kenkyū-buntansha) |
MORI Makoto Shizuoka University, 農学部, 助教授 (90143411)
TOJO Hideaki Toyama Medical-Pharmacological University, 医学部, 助教授 (20041668)
YAMAMURA Kenichi Kumamoto University, 医学部, 教授 (90115197)
YAMADA Junzo Kyoto University, 医学部, 教授 (90025651)
UTSUMI Kyozo Kyoto University, 農学部, 助教授 (90033266)
辻 荘一 神戸大学, 農学部, 助教授 (10031220)
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| Project Period (FY) |
1987 – 1988
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| Project Status |
Completed (Fiscal Year 1988)
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| Budget Amount *help |
¥10,800,000 (Direct Cost: ¥10,800,000)
Fiscal Year 1988: ¥4,100,000 (Direct Cost: ¥4,100,000)
Fiscal Year 1987: ¥6,700,000 (Direct Cost: ¥6,700,000)
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| Keywords | Gene cloning / gene mapping / in vitro fertilization / gene injection / OTC gene / Ang gene human prealbumin gene / ヒトプレアルブミン遺伝子 / 精巣移植 / 外来遺伝子の導入 / ヒトAγ / βグロビン遺伝子 / ヒト成長ホルモン遺伝子 / ラットAng´遺伝子 / ラットOTC遺伝子 / 遺伝子導入 / 成長ホルモン遺伝子 / Ornithine・trans carvamirase遺伝子 / Angiotensinogen遺伝子 / プレアルブミン遺伝子 / ^Aγ / 精巣化因子 |
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| Research Abstract |
Gene cloning and gene manipulation in culture cells and animal were carried on for the purpose of the production of transgenic animal usefull for livestock industry.
New technology for embryo manipulation and developmental biology such as in vitro fertilization, in vitro culture, embryo freezing, and for microinjection of DNA into oocytes were exploited in this program. The penetration process of sperm into poltry oocytes and the in vitro culture system of cattle oocyte in vitro fertilized were observed. By the transplantation of adult testes into poltry fetus, the gonad were differentiated into tublar structure. This suggests that an certain factor to induce the gonado to testes contain in male testes organ. To produce new marker for the reserch in gene expression, gene cloning and gene mapping in mammals and poltry were carried out. Two sorts of mRNA for OTC gene were isolated for chik liver, and Ang gene in rat was mapped on the 19th, chromosome. C-DNA of goat growth hormone gene was purified and was intoduced into mouse pronuclear embryos. Stage specific gene expression of human Ar/b globin gene was observed in transgenic mouse embryos. Expression of globin gene transgened was switched Ar to Ar to Ab, according to the developmental stage of embryos. Transgenic mouse from embryos infected human prealbumin gene had a high velel of albumin protein in the systemic blood, and so expected to use for model animal of human myloidneuropathy.
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